Targeting certain rogue T cells prevents and reverses multiple sclerosis: Mouse study

Summary: TH17 cells produced increased amounts of SerpinB1, a protein implicated in multiple sclerosis symptoms. SerpinB1 cells were identified with antibodies targeting the CXCR6 surface protein. Using monoclonal antibodies to target CXCR6, the cells disappeared significantly, and the mice primed to develop MS did not develop the disease.

Source: Boston Children’s Hospital

Multiple sclerosis, an autoimmune disorder, is known to be driven by “helper” T cells, white blood cells that mount an inflammatory attack on the brain and spinal cord. A new study from Boston Children’s Hospital pinpoints the specific subgroup of helper T cells that cause MS, as well as a protein on their surface, called CXCR6, that marks them. An antibody targeting CXCR6 both prevented and reversed MS in a mouse model, the researchers report this week in PNAS.

If the findings bear out in human studies, targeting these rogue T cells could ameliorate MS, the researchers believe. The findings could also apply to other forms of autoimmune encephalomyelitis (inflammation of the brain and spinal cord), as well as inflammatory arthritis, says Eileen Remold-O’Donnell, PhD, of the Program in Cellular and Molecular Medicine at Boston Children’s Hospital, senior author on the paper.

Remold-O’Donnell and Lifei Hou, PhD, her former postdoctoral fellow and first author on the paper, have filed a patent covering the work and have formed a company, Edelweiss Immune, Inc., in which they have equity ownership together with Boston Children’s Hospital.

“We’ve demonstrated in mice you can target these cells and get rid of them,” says Remold-O’Donnell, a principal in the company together with Hou. “We don’t know if this approach would be appropriate for all cases of MS, but it could be effective in the early inflammatory stages of the disease, and in targeting newly formed cells during disease exacerbations.”

Targeting MS-inducing cells

T helper cells, in general, have been known to drive MS, coordinating the attack on the protective myelin sheath that covers nerve fibers. But there are many different types of T helper cells. Recent studies have pointed to TH17 cells, but some TH17 cells appear not to be involved in MS.

The new study zeroed in on a subset of TH17-derived cells, all bearing the CXCR6 marker. These cells are fast-proliferating and very damaging, producing one set of proteins that directly damage cells and others, including GM-CSF, that stimulate an inflammatory attack by other immune cells known as macrophages.

The study showed that these cells also produce increased amounts of a protein called SerpinB1 (Sb1), and that this protein’s presence is necessary for MS symptoms. When Sb1 was genetically deleted in T cells in the mouse MS model, fewer immune cells survived to infiltrate the spinal cord, and the disease was ameliorated as compared with control mice. The team then went on to show that these Sb1-containing cells could be readily identified with antibodies targeting the CXCR6 surface protein.

Human counterparts

To investigate whether CXCR6-positive cells are relevant in human disease, Remold-O’Donnell and Hou worked with physicians in Boston Children’s departments of Immunology and Neurology, as well as rheumatologists at Brigham and Women’s Hospital, to obtain samples of synovial fluid (from the cavities of joints) from patients with inflammatory autoimmune arthritis. They indeed found elevated levels of CXCR6+ cells in the inflamed joints. In contrast, circulating blood from the arthritis patients did not have elevated CXCR6+ cells. Nor did the blood of patients with MS or from healthy controls.

This is a diagram of myelin

T helper cells bearing the CXCR6 surface marker drive multiple sclerosis by producing a host of proteins that damage nerve fibers, by attacking their protective myelin sheath. The cells are shown here releasing GM-CSF, which stimulates other immune cells, macrophages, to release damaging inflammatory compounds. The image is credited to Lifei Hou/Boston Children’s Hospital.

When the team used monoclonal antibodies to target CXCR6, the harmful cells largely disappeared, and mice, which were primed to get MS, did not develop the disease.

The researchers believe treatments to deplete CXCR6+ cells could mitigate MS and possibly other autoimmune disorders while largely leaving other T cell immune defenses intact. The new company, Edelweiss Immune, will be carrying the research forward.

“Many drugs have been developed to treat autoimmune diseases, such as glucocorticoids and cytotoxic reagents,” says Hou. “However, none selectively target pathogenic T cells, and long-term use of immunosuppressive agents results in broad immunosuppression and compromised immune defenses. Therapeutics with better selectivity, safety, and efficacy are needed.”

Coauthors on the study at Boston Children’s were Koichi Yuki of the Department of Anesthesiology, Critical Care and Pain Medicine; Jessica Cooley of the Program in Cellular and Molecular Medicine; Lauren Henderson and Peter Nigrovic of the Rheumatology Program; and Mark Gorman of the Department of Neurology. See the paper for a full list of authors.
Funding: The work was supported by the NIH (R21 AI117440, RO1 AR065538, KO8 AR073339, K08 AR072791, P30 AR070253) and the Swiss National Science Foundation (316030_150768, 310030_146130).

About this neuroscience research article

Source:
Boston Children’s Hospital
Media Contacts:
Kristen Dattoli – Boston Children’s Hospital
Image Source:
The image is credited to Lifei Hou/Boston Children’s Hospital.

Original Research: Closed access
“SerpinB1 controls encephalitogenic T helper cells in neuroinflammation”. Lifei Hou, Deepak A. Rao, Koichi Yuki, Jessica Cooley, Lauren A. Henderson, A. Helena Jonsson, Dion Kaiserman, Mark P. Gorman, Peter A. Nigrovic, Phillip I. Bird, Burkhard Becher, and Eileen Remold-O’Donnell.
PNAS doi:10.1073/pnas.1905762116.

Abstract

SerpinB1 controls encephalitogenic T helper cells in neuroinflammation

SerpinB1, a protease inhibitor and neutrophil survival factor, was recently linked with IL-17–expressing T cells. Here, we show that serpinB1 (Sb1) is dramatically induced in a subset of effector CD4 cells in experimental autoimmune encephalomyelitis (EAE). Despite normal T cell priming, Sb1−/− mice are resistant to EAE with a paucity of T helper (TH) cells that produce two or more of the cytokines, IFNγ, GM-CSF, and IL-17. These multiple cytokine-producing CD4 cells proliferate extremely rapidly; highly express the cytolytic granule proteins perforin-A, granzyme C (GzmC), and GzmA and surface receptors IL-23R, IL-7Rα, and IL-1R1; and can be identified by the surface marker CXCR6. In Sb1−/− mice, CXCR6+ TH cells are generated but fail to expand due to enhanced granule protease-mediated mitochondrial damage leading to suicidal cell death. Finally, anti-CXCR6 antibody treatment, like Sb1 deletion, dramatically reverts EAE, strongly indicating that the CXCR6+ T cells are the drivers of encephalitis.

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