Summary: Researchers report new findings about the immune system could help treat inflammation in Alzheimer’s disease and could save people from deadly inflammation associated with sepsis.
Source: University of Virginia Health System.
A new discovery about the immune system may allow doctors to treat harmful inflammation that damages the brain in neurodegenerative diseases such as Alzheimer’s. It might also let doctors save patients from the potentially deadly inflammation of sepsis, a full-body infection that kills a quarter-million Americans every year.
The finding “opens up a whole new research area to look at neuroinflammation in the context of Alzheimer’s and Parkinson’s,” said lead researcher Bimal Desai, PhD, of the University of Virginia School of Medicine. “But the clinical impact will be in many, many different areas.”
Traditional treatments for neurological inflammation, such as in Alzheimer’s and Parkinson’s disease, are largely ineffective because biological drugs are blocked by what is known as the blood-brain barrier. That barrier protects the brain from dangers such as bacteria or toxins in the blood, but it also makes it very difficult to get drugs into the brain. “A lot of the drugs we use right now to treat inflammation, [known as] biologicals, don’t work in the brain because they can’t get through,” explained Desai, of UVA’s Department of Pharmacology and UVA’s Carter Immunology Center.
His new finding, involving important immune cells known as macrophages (and microglia), could offer a way around that. He and his team have identified a specific electrical switch, known as an ion channel, within macrophages that controls the flow of calcium into the cells. Without calcium, the cells can’t cause inflammation. By targeting this switch with tiny molecules, researchers could deny the macrophages calcium and prevent inflammation – even in the brain.
A Better Way to Battle Inflammation
That could let researchers develop a new and better way to stop inflammation. “Small molecules are perhaps more affordable as treatments and can hit things like this ion channel switch, TRPM7,” said researcher Michael Schappe, a graduate student in Desai’s lab. “We could use that to address inflammation in a bunch of contexts, but particularly in instances like neuroinflammation, where [current] treatments are particularly ineffective.”
Desai noted that drug companies are already at work on drugs that could target this type of switch. And that could be good news for patients with many inflammatory diseases. “Right now, you have conditions like arthritis or IBD [inflammatory bowel disease], where inflammation plays a huge role. They do have very good drugs for them, but these drugs are extremely expensive and cannot be taken orally by the patients. They can cost as much as $20,000 a year,” he said. “The reason for that is that they’re biologicals. They’re protein molecules that are very difficult to make and distribute. But having identified an ion channel as a target in this context allows you to use small molecules, which are ridiculously cheap compared to biologicals and can be taken orally by the patients.”
About this neuroscience research article
The researchers have published their findings in the scientific journal Immunity. The study’s authors were Schappe, Kalina Szteyn, Marta E. Stremska, Suresh K. Mendu, Taylor K. Downs, Philip V. Seegren, Michelle A. Mahoney, Sumeet Dixit, Julia K. Krupa, Eric J. Stipes, Jason S. Rogers, Samantha E. Adamson, Norbert Leitinger and Desai.
Funding: The research was supported by the National Institutes of Health, grants GM108989 and 5T32GM007055-41.
[cbtabs][cbtab title=”MLA”]University of Virginia Health System “New Way to Stop Inflammation in Alzheimer’s and Immune System Disorders.” NeuroscienceNews. NeuroscienceNews, 26 February 2018. <https://neurosciencenews.com/inflammation-alzheimers-immune-system-8564/>.[/cbtab][cbtab title=”APA”]University of Virginia Health System (2018, February 26). New Way to Stop Inflammation in Alzheimer’s and Immune System Disorders. NeuroscienceNews. Retrieved February 26, 2018 from https://neurosciencenews.com/inflammation-alzheimers-immune-system-8564/[/cbtab][cbtab title=”Chicago”]University of Virginia Health System “New Way to Stop Inflammation in Alzheimer’s and Immune System Disorders.” https://neurosciencenews.com/inflammation-alzheimers-immune-system-8564/ (accessed February 26, 2018).[/cbtab][/cbtabs]
Chanzyme TRPM7 Mediates the Ca2+ Influx Essential for Lipopolysaccharide-Induced Toll-Like Receptor 4 Endocytosis and Macrophage Activation
Highlights •TRPM7 is essential for LPS-induced macrophage activation •TRPM7 mediates the Ca2+ influx necessary for TLR4 endocytosis •LPS-induced phosphorylation and translocation of NFκB p65 and IRF3 depend on TRPM7 •Mice with a myeloid-specific Trpm7 deletion are resistant to LPS-induced peritonitis
Summary Toll-like receptors (TLRs) sense pathogen-associated molecular patterns to activate the production of inflammatory mediators. TLR4 recognizes lipopolysaccharide (LPS) and drives the secretion of inflammatory cytokines, often contributing to sepsis. We report that transient receptor potential melastatin-like 7 (TRPM7), a non-selective but Ca2+-conducting ion channel, mediates the cytosolic Ca2+ elevations essential for LPS-induced macrophage activation. LPS triggered TRPM7-dependent Ca2+ elevations essential for TLR4 endocytosis and the subsequent activation of the transcription factor IRF3. In a parallel pathway, the Ca2+ signaling initiated by TRPM7 was also essential for the nuclear translocation of NFκB. Consequently, TRPM7-deficient macrophages exhibited major deficits in the LPS-induced transcriptional programs in that they failed to produce IL-1β and other key pro-inflammatory cytokines. In accord with these defects, mice with myeloid-specific deletion of Trpm7 are protected from LPS-induced peritonitis. Our study highlights the importance of Ca2+ signaling in macrophage activation and identifies the ion channel TRPM7 as a central component of TLR4 signaling.