Summary: Study reveals the immune system response may contribute to additional injury following damage to the spinal cord.
Source: Florida State University.
Thousands of people worldwide suffer severe spinal cord injuries each year, but little is known about why these injuries often continue to deteriorate long after the initial damage occurs.
Yi Ren, a professor of biomedical sciences at the Florida State University College of Medicine, is making progress in understanding why such significant harm is inflicted in the weeks and months after a spinal injury. In a study published today in the journal Nature Neuroscience, Ren explained how a natural immune system response may contribute to additional injury.
When spinal cord damage occurs, the endothelial cells that line blood vessels are activated to remove potentially harmful material, like myelin debris, from the site of the injury. However, Ren and her team discovered that this process may be responsible for causing further harm.
“The consequences of the effort of endothelial cells to clear myelin debris is often severe, contributing to post-traumatic degeneration of the spinal cord and to the functional disabilities often associated with spinal cord injuries,” said Ren, whose team conducted the study over a period of five years.
Myelin debris at the injury site comes from a shattering of the insulation protecting axons — the central nervous system’s primary transmission lines.
The inflamed area fills with macrophages, a type of white blood cell that engulfs foreign material and dead cells and is a key player in the immune response system. Macrophages remain in the area of the inflammation for months or even years. The mechanisms for macrophage infiltration remain unclear.
“Uncontrolled inflammation is one of the most important pathological events in the secondary injury cascade,” Ren said. “It persists for a long period of time following a spinal cord injury. We know that myelin debris acts as an inflammatory stimulus that exacerbates secondary injury by activating other cells in the injured spinal cord that are actively involved in inflammatory responses during disease progression.
“Clearing myelin debris generated at the time of injury is critical in controlling the inflammatory response and to ensuring neural regeneration.”
Little is known about the cellular and molecular mechanisms at work in clearing myelin debris from the injury site.
Ren’s lab demonstrated that debris can be engulfed by blood vessels and endothelial cells in the injured spinal cord. The problem is that once endothelial cells engulf the debris, they are enabled to promote inflammation and the formation of abnormal blood vessels. Those outcomes would inhibit the chances of a full recovery.
“Unexpectedly, we found that the process of engulfing debris confers upon endothelial cells the ability to stimulate production of fibrotic components suggesting that these cells have a function in the formation of fibrotic scars,” Ren said. “Specifically, they facilitate the arrival of macrophages derived from bone marrow that ultimately promote chronic inflammation.”
Ren said the same findings apply to the central nervous system damage inflicted by multiple sclerosis.
With a better understanding of the mechanisms at work, Ren hopes that researchers will find new ways for accident victims to regain lost functional ability without many of the unwanted side effects.
Source: Doug Carlson – Florida State University
Publisher: Organized by NeuroscienceNews.com.
Image Source: NeuroscienceNews.com image is adapted from the Florida State University news release.
Original Research: Abstract for “Microvascular endothelial cells engulf myelin debris and promote macrophage recruitment and fibrosis after neural injury” by Tian Zhou, Yiming Zheng, Li Sun, Smaranda Ruxandra Badea, Yuanhu Jin, Yang Liu, Alyssa J. Rolfe, Haitao Sun, Xi Wang, Zhijian Cheng, Zhaoshuai Huang, Na Zhao, Xin Sun, Jinhua Li, Jianqing Fan, Choogon Lee, Timothy L. Megraw, Wutian Wu, Guixue Wang & Yi Ren in Nature Neuroscience. Published January 21 2019.
Microvascular endothelial cells engulf myelin debris and promote macrophage recruitment and fibrosis after neural injury
The clearance of damaged myelin sheaths is critical to ensure functional recovery from neural injury. Here we show a previously unidentified role for microvessels and their lining endothelial cells in engulfing myelin debris in spinal cord injury (SCI) and experimental autoimmune encephalomyelitis (EAE). We demonstrate that IgG opsonization of myelin debris is required for its effective engulfment by endothelial cells and that the autophagy–lysosome pathway is crucial for degradation of engulfed myelin debris. We further show that endothelial cells exert critical functions beyond myelin clearance to promote progression of demyelination disorders by regulating macrophage infiltration, pathologic angiogenesis and fibrosis in both SCI and EAE. Unexpectedly, myelin debris engulfment induces endothelial-to-mesenchymal transition, a process that confers upon endothelial cells the ability to stimulate the endothelial-derived production of fibrotic components. Overall, our study demonstrates that the processing of myelin debris through the autophagy–lysosome pathway promotes inflammation and angiogenesis and may contribute to fibrotic scar formation.