Summary: High frequency stimulation of neural fibers leads to greater OPCs proliferation, a new study reports.
Different firing patterns of neurons alter proliferation and differentiation of oligodendrocyte precursors.
Through their pattern of firing, neurons influence the behavior of the cells that upon maturation will provide insulation of neuronal axons, according to a new study publishing 22 August in the open access journal PLOS Biology by Balint Nagy, Maria Kukley and colleagues at the University of Tübingen, Germany. The findings suggest the existence of a complex and nuanced interplay between neurons and the non-neuronal cells that support and protect them.
Oligodendrocyte precursor cells (OPCs) give rise to oligodendrocytes, which wrap the axons of neurons in the central nervous system with myelin to electrically insulate them. Neurons signal to OPCs through chemical synapses. Previous work has shown that electrical or optical stimulation of nerve fibers influences the proliferation of OPCs and their differentiation into oligodendrocytes, but whether that stimulation acts like an on-off switch, influencing OPCs only by its presence or absence, or more like a dimmer switch, inducing a graded response in the OPC, has been unclear.
To investigate that question, the authors studied oligodendroglial cells in the corpus callosum, a nerve tract which connects the two hemispheres of the brain, both in fresh brain slices and in living rodents. Using electrodes, they stimulated neurons to fire and recorded responses in the neighboring OPCs.
They found that stimulating neuronal fibers in brain slices at low frequencies led to a slowly oscillating movement of ions through the membrane of OPCs, while high-frequency stimulation caused a much more rapidly oscillating movement of ions. High-frequency stimulation of neuronal fibers in living animals led to greater OPC proliferation (creating more OPCs) over the course of a week than did low-frequency stimulation; low-frequency stimulation, in turn, led to more differentiation of OPCs into pre-myelinating oligodendrocytes, the cells that then go on to develop into oligodendrocytes which make myelin.
How OPCs translate different patterns of neuronal firing into differences in proliferation and differentiation is unknown, but differences in firing pattern have been seen to affect gene expression in neurons themselves in a graded rather than on-off way, suggesting that a similar mechanism may be at work in OPCs. Intriguingly, different neuronal firing patterns affect properties of chemical synapses between neurons and OPC, as well as intracellular concentrations of ions in OPCs such as sodium, potassium, and calcium, in a distinct fashion. Hence, it is possible that neurons use synapses with OPCs to influence OPCs behavior, including proliferation and maturation. It is not yet known whether the frequency-related differences in behavior of OPCs actually match the neuron’s respective needs.
Myelination in the brain is plastic. It can be influenced by our everyday behavior and is responsive to environmental inputs, with physical activity increasing it and social isolation decreasing it. Understanding the mechanisms mediating the effects of activity on myelination may offer significant opportunities for therapeutic intervention in disorders in which myelination or remyelination are deficient. “This research may open new perspective to therapy of demyelinating disorders where remyelination strongly relies on the increased proliferation and differentiation of OPCs,” commented Kukley.
Source: Maria Kukley – PLOS
Image Source: NeuroscienceNews.com image is credited to Balint Nagy.
Original Research: Full open access research for “Different patterns of neuronal activity trigger distinct responses of oligodendrocyte precursor cells in the corpus callosum” by Balint Nagy, Anahit Hovhannisyan, Ruxandra Barzan, Ting-Jiun Chen, and Maria Kukley in PLOS Biology. Published online August 22 2017 doi:10.1371/journal.pbio.2001993
Different patterns of neuronal activity trigger distinct responses of oligodendrocyte precursor cells in the corpus callosum
In the developing and adult brain, oligodendrocyte precursor cells (OPCs) are influenced by neuronal activity: they are involved in synaptic signaling with neurons, and their proliferation and differentiation into myelinating glia can be altered by transient changes in neuronal firing. An important question that has been unanswered is whether OPCs can discriminate different patterns of neuronal activity and respond to them in a distinct way. Here, we demonstrate in brain slices that the pattern of neuronal activity determines the functional changes triggered at synapses between axons and OPCs. Furthermore, we show that stimulation of the corpus callosum at different frequencies in vivo affects proliferation and differentiation of OPCs in a dissimilar way. Our findings suggest that neurons do not influence OPCs in “all-or-none” fashion but use their firing pattern to tune the response and behavior of these nonneuronal cells.
“Different patterns of neuronal activity trigger distinct responses of oligodendrocyte precursor cells in the corpus callosum” by Balint Nagy, Anahit Hovhannisyan, Ruxandra Barzan, Ting-Jiun Chen, and Maria Kukley in PLOS Biology. Published online August 22 2017 doi:10.1371/journal.pbio.2001993