The Race Against Zika

U-Alberta researchers seeking ways to diagnose, treat fast-spreading virus possibly linked to birth defects.

While most of us were first hearing of a strange, new and dangerous mosquito-borne virus stampeding across South and Central America and into Mexico, University of Alberta virologist Tom Hobman and his team were months deep into a plan looking for ways to diagnose and treat this threat.

“Unfortunately, there is a feeling that this is going to get a lot worse before it gets better,” says Hobman, a professor in the cell biology department of the U of A’s Faculty of Medicine & Dentistry.

What “this” happens to be is an outbreak of the Zika virus. First isolated in monkeys in Uganda in 1947, the Zika virus is a member of the flaviviruses—a classification of viruses that also includes West Nile and dengue fever virus.

Older strains of the Zika virus were not recognized to cause serious disease. In most cases, infections resulted in moderate fever with flu-like symptoms that disappeared in less than a week. Because these outbreaks were self-limiting and outcomes from infection were relatively benign, Hobman says, there was not much incentive to study the virus.

That changed in May of 2015, when an outbreak of the virus in Brazil coincided with a 2,700 per cent increase in reported cases of microcephaly, an often fatal congenital condition associated with incomplete brain development in newborns.

“If you think of the cost—the health-care and personal cost—of having a child with microcephaly, it’s enormous,” said Hobman. “In many cases, children born with this condition die within the first year, but others can live relatively long lives, even those with severe mental and physical disabilities.”

So far, there have been at least three imported cases in Canada, at a time when there are no vaccines, no treatments and the diagnostics are not overly reliable. In the United States, a concerted effort to develop a vaccine will soon be under way, but it could still be 10 years by the time the vaccine receives regulatory approval for widespread use.

Image shows the zika virus in human cells.
And although treatments with the ability to block mother-to-baby transmission have a longer time horizon, the promise for creating tools for diagnosing the virus is more immediate. Credit: Shangmei Hou.

This yet-unproven link to microcephaly, according to Hobman team member Daniel Limonta, a post-doctoral fellow funded by Alberta Innovates – Health Solutions, is “highly unusual for this group of viruses. You don’t normally see mother-to-fetus transmission.”

Limonta points out that there is a recently published study suggesting the virus can be transmitted sexually.

Compounding the problem further is that the disease spreads at an unprecedented pace. Since the Brazilian Zika virus outbreak started in May 2015, the virus is thought to have spread to every country in the Americas except Canada and Chile.

“By comparison, West Nile took years just to spread across North America,” said Hobman, whose samples of the Zika virus arrived at his lab Jan. 20. “It’s pretty incredible.”

In the wake of all this, the U.S. Centers for Disease Control and Prevention has taken the extreme measure of issuing travel advisories for pregnant women and women thinking about getting pregnant, suggesting that they not travel to more than 20 countries in the Americas where the Zika virus is endemic.

Meanwhile, Hobman’s team has begun following up on its strategy to pinpoint diagnostic tools to identify those who are infected with the virus and therapeutics to fight it.

Anil Kumar, another AIHS-funded post-doctoral fellow in Hobman’s lab, says there are libraries of approved drugs that he will be testing to see whether any of them have any antiviral activity against the virus.

This is the first super-resolution microscopy showing replication of Zika virus in a human cell. The images were taken Jan. 25, 2016. The red dots represent virus replication sites, the green structures are actin and the large blue structure is the cell nucleus.

“The great part is these drugs have already been approved, so they’re safe,” said Kumar. “We do have a good head start, as we have already done this type of work on dengue and West Nile.”

And although treatments with the ability to block mother-to-baby transmission have a longer time horizon, the promise for creating tools for diagnosing the virus is more immediate.

“There is a protein that we will make antibodies against. These antibodies are often used in a diagnostic test,” said Kumar. “This is something we can probably do within a year.”

Hobman says doing research at a university that is a powerhouse in virology has allowed his team to get a head start on this work, as well as access to some of the world’s best collaborators—many of whom are next door in the U of A’s Li Ka Shing Institute of Virology.

“I don’t think you have this collection of virology experts anywhere in the country, at least that work with these types of viruses.”

About this neuroscience research

Source: Michael Brown – University of Alberta
Image Source: The image is adapted from the video by Shangmei Hou
Video Source: The video is credited to Shangmei Hou

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