Scientists are one step closer to understanding how the brain regulates memory and mood, thanks to the discovery of two distinct types of stem cells.
University of Queensland researchers have identified two types of stem cells in the hippocampus, a region of the brain crucial for learning and memory.
Dr Dhanisha Jhaveri, the study’s lead author, said Queensland Brain Institute (QBI) researchers had isolated pure populations of these cells for the first time.
The discovery may have implications for the treatment of learning- and mood-related disorders.
“The stem cells we have identified give rise to new neurons,” Dr Jhaveri said.
“The production of new neurons in the brain, a process that decreases as we age, is essential for learning and cognitive function.”
Professor Perry Bartlett, QBI director, said the discovery solved a longstanding mystery about the birth of new neurons in the hippocampus.
“Previously, these neurons were all thought to be identical, so it wasn’t understood how the region is able to regulate behaviours as divergent as learning and mood,” he said.
“The existence of distinct stem cell populations suggests that they give rise to different types of neurons, which explains the varied functions of the hippocampus.”
Dr Jhaveri said the discovery was made using state-of-the-art cell-sorting and DNA technologies.
“The two cell groups are located in different regions of the hippocampus, which suggests that distinct areas within the hippocampus control spatial learning versus mood,” she said.
“When we purified the cells we found that they are activated by different mechanisms, and generate new neurons that differ in their gene expression.”
The study, Purification of neural precursor cells reveals the presence of distinct, stimulus-specific subpopulations of quiescent precursors in the adult mouse hippocampus, is published in the Journal of Neuroscience.
Funding: The research was funded by the National Health and Medical Council, the Australian Research Council, and the Estate of Dr Clem Jones AO.
Source: Darius Koreis – University of Queensland
Image Source: Image is in the public domain
Original Research: Abstract for “Purification of Neural Precursor Cells Reveals the Presence of Distinct, Stimulus-Specific Subpopulations of Quiescent Precursors in the Adult Mouse Hippocampus” by Dhanisha J. Jhaveri, Imogen O’Keeffe, Gregory J. Robinson, Qiong-Yi Zhao, Zong Hong Zhang, Virginia Nink, Ramesh K. Narayanan, Geoffrey W. Osborne, Naomi R. Wray, and Perry F. Bartlett in Journal of Neuroscience. Published online May 27 2015 doi:10.1523/JNEUROSCI.0504-15.2015
Abstract
Purification of Neural Precursor Cells Reveals the Presence of Distinct, Stimulus-Specific Subpopulations of Quiescent Precursors in the Adult Mouse Hippocampus
The activity of neural precursor cells in the adult hippocampus is regulated by various stimuli; however, whether these stimuli regulate the same or different precursor populations remains unknown. Here, we developed a novel cell-sorting protocol that allows the purification to homogeneity of neurosphere-forming neural precursors from the adult mouse hippocampus and examined the responsiveness of individual precursors to various stimuli using a clonal assay. We show that within the Hes5-GFP+/Nestin-GFP+/EGFR+ cell population, which comprises the majority of neurosphere-forming precursors, there are two distinct subpopulations of quiescent precursor cells, one directly activated by high-KCl depolarization, and the other activated by norepinephrine (NE). We then demonstrate that these two populations are differentially distributed along the septotemporal axis of the hippocampus, and show that the NE-responsive precursors are selectively regulated by GABA, whereas the KCl-responsive precursors are selectively modulated by corticosterone. Finally, based on RNAseq analysis by deep sequencing, we show that the progeny generated by activating NE-responsive versus KCl-responsive quiescent precursors are molecularly different. These results demonstrate that the adult hippocampus contains phenotypically similar but stimulus-specific populations of quiescent precursors, which may give rise to neural progeny with different functional capacity.
“Purification of Neural Precursor Cells Reveals the Presence of Distinct, Stimulus-Specific Subpopulations of Quiescent Precursors in the Adult Mouse Hippocampus” by Dhanisha J. Jhaveri, Imogen O’Keeffe, Gregory J. Robinson, Qiong-Yi Zhao, Zong Hong Zhang, Virginia Nink, Ramesh K. Narayanan, Geoffrey W. Osborne, Naomi R. Wray, and Perry F. Bartlett in Journal of Neuroscience. Published online May 27 2015 doi:10.1523/JNEUROSCI.0504-15.2015
