Summary: During non-REM sleep, visual areas of the brain exhibit an excitation/inhibition balance indicative of increased plasticity. REM sleep appears to be essential for people to reap the benefits of the increased plasticity that occurs during NREM sleep.
Source: Brown University
Which sleep stage is most important for learning: REM or non-REM? Does sleep improve learning by enhancing skills while people snooze, or by cementing those skills in the brain so that they’re less likely to forget them? Do these processes occur every time someone sleeps, or only after they have learned something new?
The answer to these questions, according to a new study on visual learning, is “all of the above.”
“Sleep is good for many processes in the body and mind, but the controversy was how sleep is good,” said corresponding author Yuka Sasaki, a professor of cognitive, linguistic and psychological sciences (research) at Brown University. “Do non-REM sleep and REM sleep make different contributions, or does the sleep stage not matter? We think we have one answer, because we clearly show the difference in the roles of non-REM sleep and REM sleep in visual perceptual learning.”
In the study, published in Nature Neuroscience on Monday, July 20, young adults were trained to identify a letter and the orientation of a set of lines on a textured background in two different tasks: one before sleep and one after sleep. Between the two tasks, the researchers analyzed the participants’ brain waves while they were sleeping and simultaneously measured the concentrations of two different chemicals in their brains: an excitatory neurotransmitter called glutamate and an inhibitory neurotransmitter called gamma-aminobutyric acid. Separately, the researchers performed the same analyses on people who did not participate in the visual learning tasks.
By measuring the ratio of these two chemicals in the brain — called the excitation/inhibition (E/I) balance — scientists can gather clues about the state of a particular brain area. Sometimes, such as when a brain area has a high E/I balance, neurons are actively forming new connections, which means that the brain area has a high degree of plasticity.
Conversely, when a brain area has low E/I balance, it is said to be in a state of stabilization. During stabilization, less important neural connections are pruned away, thereby increasing the efficiency and resiliency of the connections that remain. Both plasticity and stabilization are integral to the learning process: Plasticity typically translates to performance gains, and stabilization prevents new learning from being overwritten or interfered with by future learning.
This new study found that plasticity and stabilization occur during different stages of sleep.
During non-REM (NREM) sleep, the visual areas of participants’ brains exhibited an E/I balance suggestive of increased plasticity. The pattern was found even among participants who did not partake in the visual learning tasks, which means that it occurs even in the absence of learning.
However, the REM stage appears to be necessary for people to reap the benefits of the increased plasticity they exhibit during NREM sleep. During REM sleep, the chemical concentrations in participants’ brains indicated that their visual areas underwent stabilization. (This process occurred only in the participants who partook in the visual learning tasks, which suggests that, in contrast to plasticity, stabilization during sleep occurs only in the presence of learning.)
Participants who only underwent NREM sleep did not exhibit any performance gains, likely because the new, post-sleep task interfered with their learning of the pre-sleep task. Conversely, those who underwent both NREM and REM sleep exhibited significant performance gains for both the pre-sleep and post-sleep task.
“I hope this helps people realize that both non-REM sleep and REM sleep are important for learning,” Sasaki said. “When people sleep at night, there are many sleep cycles. REM sleep appears at least three, four, five times, and especially in the later part of the night. We want to have lots of REM sleep to help us remember more robustly, so we shouldn’t shorten our sleep.”
Going forward, Sasaki and her colleagues would like to see if their findings can be generalized to other types of learning. They would also like to combine this research with their past research on visual perceptual learning and reward.
“Previously, we showed that reward enhances visual learning through sleep, so we’d like to understand how that works,” she said. “It’s ambitious, but maybe we could expand this research to other types of learning so we could remember better and develop better motor learning, better visual skills and better creativity.”
In addition to Sasaki, other Brown University authors on the study were Masako Tamaki, Zhiyan Wang, Tyler Barnes-Diana, DeeAnn Guo, Aaron V. Berard, Edward Walsh and Takeo Watanabe.
Funding: The study was supported by the National Institutes of Health (R21EY028329, R01EY019466, R01EY027841, T32EY018080, T32MH115895 and BSF2016058). Part of this research was also supported by the Center for Vision Research at Brown.
About this memory research article
Kevin Stacey – Brown University
The image is in the public domain.
Original Research: Closed access
“Complementary contributions of non-REM and REM sleep to visual learning”. by Masako Tamaki, Zhiyan Wang, Tyler Barnes-Diana, DeeAnn Guo, Aaron V. Berard, Edward Walsh, Takeo Watanabe & Yuka Sasaki. Nature Neuroscience.
Complementary contributions of non-REM and REM sleep to visual learning
Sleep is beneficial for learning. However, it remains unclear whether learning is facilitated by non-rapid eye movement (NREM) sleep or by REM sleep, whether it results from plasticity increases or stabilization, and whether facilitation results from learning-specific processing. Here, we trained volunteers on a visual task and measured the excitatory and inhibitory (E/I) balance in early visual areas during subsequent sleep as an index of plasticity. The E/I balance increased during NREM sleep irrespective of whether pre-sleep learning occurred, but it was associated with post-sleep performance gains relative to pre-sleep performance. In contrast, the E/I balance decreased during REM sleep but only after pre-sleep training, and the decrease was associated with stabilization of pre-sleep learning. These findings indicate that NREM sleep promotes plasticity, leading to performance gains independent of learning, while REM sleep decreases plasticity to stabilize learning in a learning-specific manner.