Sensory Neurons Outside the Brain Drive Autistic Social Behaviors

Summary: The loss of the protein neurofibromin 1 (NF1) in adult male flies resulted in social impairments. The deficits were traced back to a primary disruption in a small group of peripheral neurons which control external stimuli, including touch and smell, that communicate to the brain.

Source: University of Pennsylvania

A new study from Penn Medicine lends further evidence that the social behaviors tied to autism spectrum disorders (ASD) emerge from abnormal function of sensory neurons outside the brain. It’s an important finding, published today in the journal Cell Reports, because peripheral sensory systems–which determine how we perceive the environment around us –makes for more accessible therapeutic targets to treat ASD-related symptoms, rather than the central brain itself.

In the fruit fly Drosophila– a powerful model for studying neurobiology– the researchers showed that loss of a protein known as neurofibromin 1 caused adult male flies to have social impairments. Those deficits, the researchers also showed, traced back to a primary disruption in a small group of peripheral neurons controlling external stimuli, like smell and touch, that communicate to the brain.

“These data raise the exciting possibility that the root of the problem doesn’t begin with errors in the brain itself. It’s the disrupted flow of information from the periphery to the brain we should be taking a closer look at,” said senior author Matthew Kayser, MD, PhD, an assistant professor in the department of Psychiatry in the Perelman School of Medicine at the University of Pennsylvania. “The findings should help guide the field toward sensory processing therapeutic targets that, if effective, could be transformative for patients suffering from these disorders.”

In humans, a loss of neurofibromin 1 is associated with neurofibromatosis type 1 (NF1), a neurodevelopmental disorder with high rates of ASD, but how that loss leads to social deficits is unknown. Past studies have also shown a link between the peripheral sensory system and social deficits; however, this is the first study to implicate neurofibromin’s function.

Up to 50 percent of children with NF1 fall on the autism spectrum, and are 13 times more likely to exhibit highly elevated ASD symptoms, including social and communicative disabilities, increased isolation and bullying, difficulties on social tasks, and sensitivities to sound or light. Those symptoms are all tied to difficulties with processing sensory information. Face and gaze processing, for example, makes a social gesture like eye contact exceedingly difficult.

The team, led by Penn postdoctoral scientist Emilia Moscato, PhD, used genetically manipulated flies to show that a loss of neurofibromin led to diminished social courtship behavior and errors in gustatory sensory neurons called ppk23, which are known to coordinate such behaviors. These behavioral deficits stem from an ongoing role for neurofibromin in coordinating social functions in adults, as opposed to guiding development of social behavioral neural circuits.

More specifically, in vivo monitoring of neural activity in the mutant flies showed decreased sensory neuron activation in response to specific pheromonal cues, which then disrupted proper function of downstream brain neurons that direct social decisions. The disruption also led to persistent changes in behavior of the flies beyond the social interaction itself, suggesting a brief sensory error can have long-lasting consequences on behavior.

This shows a child playing with building blocks
Next, the researchers aim to better understand how this mutation translates to disruption in brain activity and ultimately behaviors associated with ASD and NF1. Image is in the public domain.

Next, the researchers aim to better understand how this mutation translates to disruption in brain activity and ultimately behaviors associated with ASD and NF1. They also hope to test different drugs in animal models to identify novel compounds that can restore social behaviors.

“Sensory processing is a readily testable entry-point into social behavioral dysfunction,” Kayser said, “so findings from these experiments have potential to rapidly impact the clinical setting.”

Co-authors of the study include Penn’s Moscato and Christine Dubowy, as well as James A. Walker of Massachusetts General Hospital.

Funding: This work was supported by a McMorris Autism Early Intervention Initiative Fund Pilot Study Award, a Burroughs Wellcome Career Award for Medical Scientists, a National Institutes of Health grant (DP2 NS111996), and an NIH New Program Development Award of the Intellectual and Developmental Disabilities Research Center at Children’s Hospital of Philadelphia/Penn (U54 HD086984).

About this autism research article

Source:
University of Pennsylvania
Media Contacts:
Hannah Messinger – University of Pennsylvania
Image Source:
The image is in the public domain.

Original Research: Open access
“Social Behavioral Deficits with Loss of Neurofibromin Emerge from Peripheral Chemosensory Neuron Dysfunction” by Emilia H. Moscato, Christine Dubowy, James A. Walker, Matthew S. Kaysery. Cell Reports


Abstract

Social Behavioral Deficits with Loss of Neurofibromin Emerge from Peripheral Chemosensory Neuron Dysfunction

Neurofibromatosis type 1 (NF1) is a neurodevelopmental disorder associated with social and communicative disabilities. The cellular and circuit mechanisms by which loss of neurofibromin 1 (Nf1) results in social deficits are unknown. Here, we identify social behavioral dysregulation with Nf1 loss in Drosophila. These deficits map to primary dysfunction of a group of peripheral sensory neurons. Nf1 regulation of Ras signaling in adult ppk23+ chemosensory cells is required for normal social behaviors in flies. Loss of Nf1 attenuates ppk23+ neuronal activity in response to pheromones, and circuit-specific manipulation of Nf1 expression or neuronal activity in ppk23+ neurons rescues social deficits. This disrupted sensory processing gives rise to persistent changes in behavior beyond the social interaction, indicating a sustained effect of an acute sensory misperception. Together our data identify a specific circuit mechanism through which Nf1 regulates social behaviors and suggest social deficits in NF1 arise from propagation of sensory misinformation.

Join our Newsletter
I agree to have my personal information transferred to AWeber for Neuroscience Newsletter ( more information )
Sign up to receive the latest neuroscience headlines and summaries sent to your email daily from NeuroscienceNews.com
We hate spam and only use your email to contact you about newsletters. We do not sell email addresses. You can cancel your subscription any time.