Gene Linked to Mitochondrial Dysfunction in Obesity

Summary: Researchers made a breakthrough in understanding obesity’s impact on mitochondria, as detailed in a recent study.

They discovered that a high-fat diet causes fat cell mitochondria in mice to fragment into smaller, less efficient units, a process controlled by a single gene. By deleting this gene, the mice were protected from weight gain despite consuming the same high-fat diet.

This study offers new insights into the metabolic dysfunctions in obesity, paving the way for potential targeted therapies.

Key Facts:

  1. The study revealed that a high-fat diet leads to the fragmentation of mitochondria in fat cells, reducing their ability to burn fat.
  2. A single gene, associated with the molecule RaIA, was found to be responsible for this mitochondrial fragmentation and metabolic disruption in obesity.
  3. By removing this gene, researchers successfully protected mice from obesity induced by a high-fat diet, suggesting a new therapeutic target for obesity treatment in humans.

Source: UCSD

The number of people with obesity has nearly tripled since 1975, resulting in a worldwide epidemic. While lifestyle factors like diet and exercise play a role in the development and progression of obesity, scientists have come to understand that obesity is also associated with intrinsic metabolic abnormalities.

Now, researchers from the University of California San Diego School of Medicine have shed new light on how obesity affects our mitochondria, the all-important energy-producing structures of our cells.

This shows DNA.
How these metabolic abnormalities start is among the biggest mysteries surrounding obesity. Credit: Neuroscience News

In a study published January 29, 2023 in Nature Metabolism, the researchers found that when mice were fed a high-fat diet, mitochondria within their fat cells broke apart into smaller mitochondria with reduced capacity for burning fat. Further, they discovered that this process is controlled by a single gene. By deleting this gene from the mice, they were able to protect them from excess weight gain, even when they ate the same high-fat diet as other mice.

“Caloric overload from overeating can lead to weight gain and also triggers a metabolic cascade that reduces energy burning, making obesity even worse,” said Alan Saltiel, PhD, professor in the Department of Medicine at UC San Diego School of Medicine. “The gene we identified is a critical part of that transition from healthy weight to obesity.”

Obesity, which affects more than 40% of adults in the United States, occurs when the body accumulates too much fat, which is primarily stored in adipose tissue. Adipose tissue normally provides important mechanical benefits by cushioning vital organs and providing insulation. It also has important metabolic functions, such as releasing hormones and other cellular signaling molecules that instruct other tissues to burn or store energy.

In the case of caloric imbalances like obesity, the ability of fat cells to burn energy starts to fail, which is one reason why it can be difficult for people with obesity to lose weight. How these metabolic abnormalities start is among the biggest mysteries surrounding obesity.

To answer this question, the researchers fed mice a high-fat diet and measured the impact of this diet on their fat cells’ mitochondria, structures within cells that help burn fat. They discovered an unusual phenomenon. After consuming a high-fat diet, mitochondria in parts of the mice’s adipose tissue underwent fragmentation, splitting into many smaller, ineffective mitochondria that burned less fat.

In addition to discovering this metabolic effect, they also discovered that it is driven by the activity of single molecule, called RaIA. RaIA has many functions, including helping break down mitochondria when they malfunction. The new research suggests that when this molecule is overactive, it interferes with the normal functioning of mitochondria, triggering the metabolic issues associated with obesity.

“In essence, chronic activation of RaIA appears to play a critical role in suppressing energy expenditure in obese adipose tissue,” said Saltiel. “By understanding this mechanism, we’re one step closer to developing targeted therapies that could address weight gain and associated metabolic dysfunctions by increasing fat burning.”

By deleting the gene associated with RaIA, the researchers were able to protect the mice against diet-induced weight gain. Delving deeper into the biochemistry at play, the researchers found that some of the proteins affected by RaIA in mice are analogous to human proteins that are associated with obesity and insulin resistance, suggesting that similar mechanisms may be driving human obesity.

“The direct comparison between the fundamental biology we’ve discovered and real clinical outcomes underscores the relevance of the findings to humans and suggests we may be able to help treat or prevent obesity by targeting the RaIA pathway with new therapies,” said Saltiel.

“We’re only just beginning to understand the complex metabolism of this disease, but the future possibilities are exciting.”

Co-authors of the study include: Wenmin Xia, Preethi Veeragandham, Yu Cao Yayun Xu, Torrey Rhyne, Jiaxin Qian, Ying Jones,Chao-Wei Hung, Zichen Wang, Hiroyuki Hakozaki and Johannes Schoneberg at UC San Diego, Peng Zhao at University of Texas Health Science Center, Hui Gao and Mikael Ryden at Karolinska Institute, Christopher Liddle, Ruth Yu, Michael Downes, Ronald Evans and Jianfeng Huang at Salk Institute for Biological Studies,Martin Wabitsch at Ulm University Medical Center and Shannon Reilly at Weill Medical College of Cornell University.

Funding: This study was funded, in part, by the National Institutes of Health (Grants P30DK063491, R01DK122804, R01DK124496, R01DK125820 and R01DK128796).

About this genetics and obesity research news

Author: Miles Martin
Source: UCSD
Contact: Miles Martin – UCSD
Image: The image is credited to Neuroscience News

Original Research: Open access.
Obesity causes mitochondrial fragmentation and dysfunction in white adipocytes due to RalA activation” by Alan Saltiel et al. Nature Metabolism


Abstract

Obesity causes mitochondrial fragmentation and dysfunction in white adipocytes due to RalA activation

Mitochondrial dysfunction is a characteristic trait of human and rodent obesity, insulin resistance and fatty liver disease. Here we show that high-fat diet (HFD) feeding causes mitochondrial fragmentation in inguinal white adipocytes from male mice, leading to reduced oxidative capacity by a process dependent on the small GTPase RalA.

RalA expression and activity are increased in white adipocytes after HFD. Targeted deletion of RalA in white adipocytes prevents fragmentation of mitochondria and diminishes HFD-induced weight gain by increasing fatty acid oxidation.

Mechanistically, RalA increases fission in adipocytes by reversing the inhibitory Ser637 phosphorylation of the fission protein Drp1, leading to more mitochondrial fragmentation. Adipose tissue expression of the human homolog of Drp1, DNM1L, is positively correlated with obesity and insulin resistance.

Thus, chronic activation of RalA plays a key role in repressing energy expenditure in obese adipose tissue by shifting the balance of mitochondrial dynamics toward excessive fission, contributing to weight gain and metabolic dysfunction.

Join our Newsletter
I agree to have my personal information transferred to AWeber for Neuroscience Newsletter ( more information )
Sign up to receive our recent neuroscience headlines and summaries sent to your email once a day, totally free.
We hate spam and only use your email to contact you about newsletters. You can cancel your subscription any time.
  1. In MICE. Mice don’t eat a high fat diet naturally. The researchers who come up with these studies make me want to stab myself in the eyeball. The media who report on it are just making suckers out of their lazy readers for clicks. All of you set REAL science back by coming up with crappy research ideas and foster a lack of trust in the public. Shame, shame, shame.

Your email address will not be published. Required fields are marked *