Summary: In response to injury, microglia cross the spinal boundary from the central nervous system to the peripheral nervous system. While in the PNS, microglia provide the function of clearing cellular debris at the point of injury, then return to the CNS in an altered state. Researchers propose this could account for some damage associated with neurodegenerative diseases.
Source: Notre Dame University
Inside the body, disease and injury can leave behind quite the mess — a scattering of cellular debris, like bits of broken glass, rubber, and steel left behind in a car accident.
Inside the central nervous system (CNS), a region that includes the brain and spinal cord, it is the job of certain cells, called microglia, to clean up that cellular debris. Microglia have counterparts called macrophages that serve similar function outside the CNS in the peripheral nervous system (PNS), the region that contains most of the sensory and motor nerves.
Scientists have long believed that microglia are restricted to the CNS, and in cases of injury the two cells clean up their own sides of the highway, so to speak.
In a new study published in the Public Library of Science (PLoS Biology), scientists at the University of Notre Dame discovered microglia actually squeeze through the spinal boundary, crossing into the peripheral nervous system in response to injury. The surprising result could have broad implications in the area of nervous system diseases while opening the door to a completely new set of questions in the study of both systems.
“Microglia are defined as central nervous system cells. So if they’re seen outside in the peripheral nervous system — that was surprising to us — that opens up a ton of new questions,” said Cody J. Smith, the Elizabeth, and Michael Gallagher Assistant Professor of Biological Sciences at Notre Dame and at the University’s Center for Stem Cells and Regenerative Medicine.
“It has been shown during different disease states that the macrophages in the peripheral can get into the CNS, but we certainly didn’t know or really expect for the central nervous system cells to cross over, because there was little literature that suggested that was likely.” Smith co-authored the study with Lauren Green, who is currently studying biology at Notre Dame and led the study.
In the study, Smith and his team modeled a brachial plexus injury in zebrafish and observed how microglia and macrophages responded. Brachial plexus injuries take place at the intersection of the central and peripheral nervous systems, affecting nerves that connect the brain and spinal cord to the shoulders, arms and hands. According to the National Institutes of Health, brachial plexus injuries occur in one to three out of every 1,000 births.
Once inside the peripheral nervous system, Smith said, microglia do their job of clearing cellular debris at the point of injury, but they return to the CNS with that debris, and could potentially carry it straight to the brain.
The study also showed the microglia return to the CNS in an altered state, which makes the results of this research of particular concern to the study of post-injury development and function.
Altered microglia have been seen widespread in countless neurodegenerative diseases, Smith said, and have been implicated in autism spectrum disorder.
In disease, altered microglial cells can clear too much cellular material — including material they normally do not clear — in the brain. If microglia are altered, the suggestion is that it could cause neuropathic pain, disorder or disease-type states, because the cells are clearing or removing cellular material that’s necessary for proper nervous system function.
“There was little thought these cells could leave the central nervous system, so there are few studies of microglia in the context of diseases and function within both central and peripheral nervous system diseases,” Smith said. “What happens when they do go into the brain after being in the PNS? What else are they capable of doing? Our study shows the full function, the full capability of these cells is not limited to the central nervous system. It opens up so many more exciting questions than it answers.”
Microglia are central nervous system (CNS)-resident cells. Their ability to migrate outside of the CNS, however, is not understood. Using time-lapse imaging in an obstetrical brachial plexus injury (OBPI) model, we show that microglia squeeze through the spinal boundary and emigrate to peripheral spinal roots. Although both macrophages and microglia respond, microglia are the debris-clearing cell. Once outside the CNS, microglia re-enter the spinal cord in an altered state. These peripheral nervous system (PNS)-experienced microglia can travel to distal CNS areas from the injury site, including the brain, with debris. This emigration is balanced by two mechanisms—induced emigration via N-methyl-D-aspartate receptor (NMDA) dependence and restriction via contact-dependent cellular repulsion with macrophages. These discoveries open the possibility that microglia can migrate outside of their textbook-defined regions in disease states.