Summary: Researchers have identified a mechanism triggered at neuron birth to intrinsically decrease apoptosis. When the genetic regulation is halted, continuous neuronal survival is disrupted, leading to a decrease in animal survival.
When our neurons — the principle cells of the brain — die, so do we.
Most neurons are created during embryonic development and have no “backup” after birth. Researchers have generally believed that their survival is determined nearly extrinsically, or by outside forces, such as the tissues and cells that neurons supply with nerve cells.
A research team led by Sika Zheng, a biomedical scientist at the University of California, Riverside, has challenged this notion and reports the continuous survival of neurons is also intrinsically programmed during development.
The study, published in the journal Neuron, identifies a mechanism the researchers say is triggered at neuron birth to intrinsically decrease a general form of cell death — or “apoptosis” — specifically in neurons. When this genetic regulation is stopped, continuous neuronal survival is disrupted and leads to the death of the animal.
An organism’s survival, brain function, and fitness are dependent upon the survival of its neurons. In higher organisms, neurons control breathing, feeding, sensation, motion, memory, emotion, and cognition. They can die of many unnatural causes, such as neurodegenerative diseases, injury, infection, and trauma. Neurons are long-lived cells, but the genetic controls that enable their longevity are unknown.
Zheng’s team now reports the central piece of the mechanism involved is a small piece of genetic sequence in Bak1, a pro-apoptotic gene whose activation leads to apoptosis. Bak1 expression is turned off when this small piece of genetic sequence, termed microexon, is spliced in the final Bak1 gene product. Exons are sequences that make up messenger RNA.
“Apoptosis is a pathway that controls cell turnover and tissue homeostasis in all metazoans,” explained Zheng, an associate professor of biomedical sciences. “Most non-neural cells readily engage in apoptosis in response to intrinsic and extrinsic stress. But this cellular suicidal program needs to be reined in for neurons so that they live for many years. We now show how genetic attenuation of neuronal apoptosis takes place.”
Zheng’s team identified the Bak1 microexon through a large-scale analysis of expression data from human tissues, mouse tissues, human developing brains, mouse developing forebrains, and mouse developing midbrains. The team first compared neural tissues with non-neural tissues in both humans and mice to identify neural-specific exons. Then, they found cortical neurons reduce their sensitivity to apoptosis as early as neuron birth. They also found apoptosis is gradually reduced during neuronal development before neurons make connections or innervate other cells, suggesting factors other than extrinsic signals can play a role.
“We show neurons transform how they regulate cell death during development,” Zheng said. “This is to ensure neuronal longevity, which is needed to maintain the integrity of neural circuits for brain functions.”
Next, Zheng’s team will study whether the identified mechanism is activated in neurodegenerative diseases and injury that cause neuronal cell death.
Zheng was joined in the research by Lin Lin and Min Zhang at UCR; Peter Stoilov at West Virginia University; and Liang Chen at the University of Southern California.
Funding: Zheng was supported by grants from the National Institutes of Health.
Developmental Attenuation of Neuronal Apoptosis by Neural-Specific Splicing of Bak1 Microexon
Highlights • Intrinsic attenuation of apoptosis starts at neuron birth with Bak1 exon 5 splicing • Bak1 exon 5 inclusion induces NMD and unproductive translation of Bak1 transcripts • Neural-specific splicing of Bak1 exon 5 enables cellular longevity of neurons • Apoptosis attenuation is essential for brain development and animal survival
Summary Continuous neuronal survival is vital for mammals because mammalian brains have limited regeneration capability. After neurogenesis, suppression of apoptosis is needed to ensure a neuron’s long-term survival. Here we describe a robust genetic program that intrinsically attenuates apoptosis competence in neurons. Developmental downregulation of the splicing regulator PTBP1 in immature neurons allows neural-specific splicing of the evolutionarily conserved Bak1 microexon 5. Exon 5 inclusion triggers nonsense-mediated mRNA decay (NMD) and unproductive translation of Bak1 transcripts (N-Bak mRNA), leading to suppression of pro-apoptotic BAK1 proteins and allowing neurons to reduce apoptosis. Germline heterozygous ablation of exon 5 increases BAK1 proteins exclusively in the brain, inflates neuronal apoptosis, and leads to early postnatal mortality. Therefore, neural-specific exon 5 splicing and depletion of BAK1 proteins uniquely repress neuronal apoptosis. Although apoptosis is important for development, attenuation of apoptosis competence through neural-specific splicing of the Bak1 microexon is essential for neuronal and animal survival.