This shows two heads.
For this study, the team explored how inducing inflammation would affect gene regulation of these taste receptors. Credit: Neuroscience News

The Bitter Truth: Illness, Inflammation, and the Genetic Code of Taste

Summary: A new study delves into the relationship between taste perception and immune function, focusing on the bitter taste experienced during illness.

The study identified that inflammation elevates aversion to bitter tastes, a change stemming from the taste buds. Utilizing a novel sequencing technique, they explored epigenetic mechanisms that influence taste-cell gene expression.

The findings have potential implications in understanding lingering bitter tastes and designing better bitter blockers for medications.

Key Facts:

  1. Bitter taste during illness may be due to how our genes in taste cells respond to inflammation.
  2. Using lipopolysaccharide (LPS), the study found that inflammation causes mice to have an elevated aversion to bitter tastes.
  3. The team utilized a single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) for the first time in this context, revealing that the bitterness response is influenced by epigenetic mechanisms.

Source: Monell Chemical Senses Center

A bitter taste in the mouth is often a symptom or side effect of illness, which may be the result of how the body reacts to pathogens.

A new study published in iScience, by Hong Wang, PhD, an Associate Member at the Monell Chemical Sense Center, and colleagues sheds light on the mechanisms involved in the complex interplay between taste perception and immune function.

Their work also highlights the potential of a sequencing tool for investigating epigenetic mechanisms that affect taste-cell gene expression. Epigenetics is the study of how and when genes are expressed rather than alteration of the genetic code itself.

In addition to being unpleasant, a bitter taste in the mouth or from food can contribute to a loss of appetite, an effect associated with ailments from the common cold to cancer. Bitter taste can also affect patients’ willingness to take certain medications, especially when they are young children.

Bitter receptors are encoded by Tas2r genes, which also provide an important defense against bacteria and parasites in the mouth and gut. However, this process is not well understood.

For this study, the team explored how inducing inflammation would affect gene regulation of these taste receptors. Using lipopolysaccharide (LPS), a compound that induces inflammation similar to that caused by bacterial infections, they found that mice showed a distinct elevated aversion to bitter tastes.

The team used nerve-recording experiments to confirm that this aversion originates in the taste buds of mice, rather than in their brains.

“Our study had very clear data showing this is actually a change at the peripheral level, not deep in the brain,” said Wang, confirming that genes in taste cells govern bitter taste distortion to this type of inflammation.

This finding has interesting clinical implications for the study of behavioral aspects of illness, such as a loss of appetite. When people are sick they often do not feel like eating. This can affect even humans’ love for sugary treats, as other studies have noted.

Mice also have a decreased preference for sweet tastes during illness and forced intake of sugar can make them sicker. These results potentially indicate a protective behavior with a biological or evolutionary basis.

To investigate the underlying gene expression mechanisms of the bitterness response, the team used several methods of analysis.

Real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) revealed a significantly increased response across the majority of the Tas2r taste-receptor genes, with peak gene expression ranging from three to five days during the sickness period.

The researchers also used single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) to explore the expression of Tas2r genes in response to LPS – the first reported instance of this method to study taste receptor gene expression.

LPS markedly increased the accessibility of many Tas2r genes, indicating that the bitter taste distortion in this experiment is caused by an epigenetic mechanism, similar to how disease-causing bacteria can affect those genes.

Finally, the study showed that LPS-induced inflammation globally affected gene expression in taste stem cells, suggesting a “remodeling” of the cells’ genome. This may leave an epigenetic memory, enabling the cells to respond faster to future infections, but may also contribute to long-lasting effects on taste responses.

This finding sheds light on why cancer treatment and certain chronic illnesses can cause a lingering bitter taste in the mouth or alter the taste perception of certain foods.

This diverse response across taste receptors has potential implications for research on how to make more effective bitter blockers for medications and other edible health and wellness products.

“The spectrum of the bitter taste receptor expression is not uniform,” Wang said.

“If we want to look at a bitter blocker for an individual taste receptor, we may want to take these factors into consideration, such as whether it’s for after a sickness, during a sickness, or which of the taste receptor genes is most prominently expressed.”

About this genetics, inflammation, and taste perception research news

Author: Karen Kreeger
Source: Monell Chemical Senses Center
Contact: Karen Kreeger – Monell Chemical Senses Center
Image: The image is credited to Neuroscience News

Original Research: Closed access.
Lipopolysaccharide increases bitter taste sensitivity via epigenetic changes in Tas2r gene clusters” by Hong Wang et al. iScience


Lipopolysaccharide increases bitter taste sensitivity via epigenetic changes in Tas2r gene clusters

People often confuse smell loss with taste loss, so it is unclear how much gustatory function is reduced in patients self-reporting taste loss. Our pre-registered cross-sectional study design included an online survey in 12 languages with instructions for self-administering chemosensory tests with 10 household items.

Between June 2020 and March 2021, 10,953 individuals participated. Of these, 5,225 self-reported a respiratory illness and were grouped based on their reported COVID test results: COVID-positive (COVID+, N = 3,356), COVID-negative (COVID−, N = 602), and COVID unknown for those waiting for a test result (COVID?, N = 1,267).

The participants who reported no respiratory illness were grouped by symptoms: sudden smell/taste changes (STC, N = 4,445), other symptoms excluding smell or taste changes (OthS, N = 832), and no symptoms (NoS, N = 416).

Taste, smell, and oral irritation intensities and self-assessed abilities were rated on visual analog scales. Compared to the NoS group, COVID+ was associated with a 21% reduction in taste (95% confidence interval (CI): 15–28%), 47% in smell (95% CI: 37–56%), and 17% in oral irritation (95% CI: 10–25%) intensity.

There were medium to strong correlations between perceived intensities and self-reported abilities (r = 0.84 for smell, r = 0.68 for taste, and r = 0.37 for oral irritation). Our study demonstrates that COVID-19-positive individuals report taste dysfunction when self-tested with stimuli that have little to none olfactory components.

Assessing the smell and taste intensity of household items is a promising, cost-effective screening tool that complements self-reports and may help to disentangle taste loss from smell loss. However, it does not replace standardized validated psychophysical tests.

Join our Newsletter
I agree to have my personal information transferred to AWeber for Neuroscience Newsletter ( more information )
Sign up to receive our recent neuroscience headlines and summaries sent to your email once a day, totally free.
We hate spam and only use your email to contact you about newsletters. You can cancel your subscription any time.