Summary: CRISPR technology allows researchers to illuminate and monitor alpha synuclein in the brain.
Source: University of Central Florida.
A team of researchers at the University of Central Florida is using breakthrough gene-editing technology to develop a new screening tool for Parkinson’s disease, a debilitating degenerative disorder of the nervous system. The technology allows scientists in the lab to “light up” and then monitor a brain protein called alpha-synuclein that has been associated with Parkinson’s.
“Alpha-synuclein is a protein that is normally found in the brain. We all have it,” said Burnett School of Biomedical Sciences doctoral student Levi Adams, one of the lead researchers on the project. “But for some reason, when you have Parkinson’s the levels become abnormal. So if we can monitor this protein in the cell, we can start to measure what causes it to go up and also what treatments can cause it to go down.”
The team published its findings in the Scientific Reports journal. The National Institutes of Health (5R21NS088923-02 ) funded the work. The researchers believe their work is a crucial step toward identifying new drug therapies for Parkinson’s disease.
Adams is partnering with doctoral student Sambuddha Basu, associate professor and neurosciences researcher, Associate Professor Yoon-Seong Kim, and scientist Subhrangshu Guhathakurta to study Parkinson’s, which affects motor functions caused by a gradual loss of brain cells. There are about 60,000 new cases of Parkinson’s each year in the United States.
They are using CRISPR Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) gene-editing technology. The system is one of research’s fastest growing biomedical techniques that allows scientists to make specific changes in the DNA of plants and animals while not killing cells. The system is becoming instrumental in studying genetically based treatments for diseases including cancer and Parkinson’s.
“It’s the most powerful and widely used gene-editing technique in use because it allows us to change the DNA in living cells,” said Kim, who is also a medical doctor. “The innovation of this method is that it enables us to monitor this gene in real-time without killing the cell. Without the CRISPR Cas-9 method, you would have to extract all the proteins from the cell to measure them, which kills the cell.”
Using the CRISPR technique, the Burnett team edited the alpha-synuclein gene and inserted a luminescent tag made from proteins that causes fireflies to light up. Every time the cell creates the alpha-synuclein protein, the tag gives off a light. That reaction “makes it much easier to measure,” Adams said. “More light means an increased level of alpha-synuclein, which would be considered a diseased state.”
The team found that measuring light was a reliable method to measure alpha-synuclein production.
“If we take one of these modified cells and treat it with a particular drug, if it doesn’t produce light anymore, then this means the drug is a potential treatment for this disease,” Basu said.
With the engineered cells, researchers can screen new and existing drugs to see how they regulate alpha-synuclein level in patients.
“With an easy-to-measure reporter like light production, this will allow us to do high throughput screening, where you can test a large panel of drugs at once,” Guhathakurta said.
With the new technology, the scientists hope to identifying ways to reduce alpha-synuclein production that can possibly prevent Parkinson’s or its progression in patients diagnosed with the disease.
The team said research will focus on what aspects of the alpha- synuclein protein kill neurons during Parkinson’s disease.
Funding: The study was funded by National Institutes of Health.
Source: Zenaida Gonzalez Kotala – University of Central Florida
Image Source: NeuroscienceNews.com image is in the public domain.
Original Research: Full open access research for “A novel tool for monitoring endogenous alpha-synuclein transcription by NanoLuciferase tag insertion at the 3′end using CRISPR-Cas9 genome editing technique” by Sambuddha Basu, Levi Adams, Subhrangshu Guhathakurta & Yoon-Seong Kim in Scientific Reports. Published online April 4 2017 doi:10.1038/srep45883
A novel tool for monitoring endogenous alpha-synuclein transcription by NanoLuciferase tag insertion at the 3′end using CRISPR-Cas9 genome editing technique
α-synuclein (α-SYN) is a major pathologic contributor to Parkinson’s disease (PD). Multiplication of α-SYN encoding gene (SNCA) is correlated with early onset of the disease underlining the significance of its transcriptional regulation. Thus, monitoring endogenous transcription of SNCA is of utmost importance to understand PD pathology. We developed a stable cell line expressing α-SYN endogenously tagged with NanoLuc luciferase reporter using CRISPR/Cas9-mediated genome editing. This allows efficient measurement of transcriptional activity of α-SYN in its native epigenetic landscape which is not achievable using exogenous transfection-based luciferase reporter assays. The NanoLuc activity faithfully monitored the transcriptional regulation of SNCA following treatment with different drugs known to regulate α-SYN expression; while exogenous promoter-reporter assays failed to reproduce the similar outcomes. To our knowledge, this is the first report showing endogenous monitoring of α-SYN transcription, thus making it an efficient drug screening tool that can be used for therapeutic intervention in PD.
“A novel tool for monitoring endogenous alpha-synuclein transcription by NanoLuciferase tag insertion at the 3′end using CRISPR-Cas9 genome editing technique” by Sambuddha Basu, Levi Adams, Subhrangshu Guhathakurta & Yoon-Seong Kim in Scientific Reports. Published online April 4 2017 doi:10.1038/srep45883