Neuroscience research articles are provided.
What is neuroscience? Neuroscience is the scientific study of nervous systems. Neuroscience can involve research from many branches of science including those involving neurology, brain science, neurobiology, psychology, computer science, artificial intelligence, statistics, prosthetics, neuroimaging, engineering, medicine, physics, mathematics, pharmacology, electrophysiology, biology, robotics and technology.
– These articles focus mainly on neurology research. – What is neurology? – Definition of neurology: a science involved in the study of the nervous systems, especially of the diseases and disorders affecting them. – Neurology research can include information involving brain research, neurological disorders, medicine, brain cancer, peripheral nervous systems, central nervous systems, nerve damage, brain tumors, seizures, neurosurgery, electrophysiology, BMI, brain injuries, paralysis and spinal cord treatments.
What is Psychology? Definition of Psychology: Psychology is the study of behavior in an individual, or group. Psychology news articles are listed below.
Artificial Intelligence articles involve programming, neural engineering, artificial neural networks, artificial life, a-life, floyds, boids, emergence, machine learning, neuralbots, neuralrobotics, computational neuroscience and more involving A.I. research.
Robotics articles will cover robotics research press releases. Robotics news from universities, labs, researchers, engineers, students, high schools, conventions, competitions and more are posted and welcome.
Genetics articles related to neuroscience research will be listed here.
Neurotechnology research articles deal with robotics, AI, deep learning, machine learning, Brain Computer Interfaces, neuroprosthetics, neural implants and more. Read the latest neurotech news articles below.
Summary: OTOP1 has been confirmed as a sour taste receptor. The protein is essential and sufficient for sour taste receptor cells to respond to acids and stimulate the nerves that enable sour taste perception.
Sour is the taste of summer, a taste that evokes lemonade stands and vine-ripe tomatoes. Among the five basic tastes — the others being bitter, sweet, salty and umami — it is arguably the most subtle. In small amounts, it adds a critical tang to an otherwise bland dish. At higher concentrations and on its own, it’s unpleasant or even painful.
But what causes the sensation of sourness, and why do we like it so much? USC scientists may have solved the first mystery: how sour tastes are sensed by animals.
Fruits and vegetables that taste sour are high in acids, including citric acid for lemons, tartaric acid for grapes and acetic acids in fermented foods like vinegar. It has been recognized for more than a century — since the introduction of the pH meter — that the low pH and high concentration of H+ ions in these foods generate a perception of sourness in humans. But how pH is sensed at the level of the tongue, and specifically what molecule constitutes the pH sensor, was not known.
A group led by Emily Liman, professor of biological sciences at the USC Dornsife College of Letters, Arts & Sciences, have reported in Current Biology that a sensor for pH on the tongue is the otopetrin 1 gene (Otop1). Otop1 is a member of a class of molecules called ion channels, which allow charged ions to cross cell membranes. In the case of Otop1, the charged ion carried across the membrane is H+, which is released into the mouth by acids.
Last year, Liman’s team published research in Science that closed in on the sour-taste sensor. In that study, they used high-throughput sequencing methods made possible by advances in genomics to identify a list of roughly 40 previously uncharacterized genes that could encode a sour sensor. By studying the function of each gene, they whittled the list down to Otop1 because it was the only candidate that, when introduced into non-taste cells, gave them the ability to respond to acids.
While the USC scientists had identified OTOP1 — the protein encoded by the Otop1 gene — as forming a proton channel, they did not show that it was required for sour-taste responses in an intact animal.
Taste occurs when ingested chemicals interact with specialized cells on the tongue and palate. These cells are called taste receptor cells and are found in taste buds, which are concentrated on the back, sides and front of the tongue and on the roof of the mouth. Different taste receptor cells respond to each of the five basic tastes, and they release neurotransmitters onto gustatory nerves that send signals to the brain. This allows the nervous system to determine whether the ingested chemical has qualities perceived as bitter, sweet, umami, sour, salty or a mix of the five.
The new study followed up on previous findings that OTOP1 gave cells the ability to detect low pH. Graduate student Yu-Hsiang Tu used gene-editing technology to generate mice with an inactivated Otop1 gene to test if the OTOP1 protein was necessary for responding to sour chemicals — or acids. When sour taste receptor cells are exposed to acids, they respond by producing an electrical signal — or current — because of the movement of H+ ions across the cell’s membrane.
Graduate student Bochuan Teng showed that the sour taste receptor cells from the mice with nonfunctional OTOP1 did not have detectable currents representing the movement of H+ into cells. The sour taste receptor cells from the mutant mice also did not decrease their intracellular pH when exposed to acids, which would happen if H+ ions moved into the cell. Finally, the sour taste receptor cells from the mutant mice did not produce action potentials — another electrical signal — which are needed to activate the gustatory nerve and signal to the brain in response to some acidic solutions.
Whereas the previous experiments were performed with isolated taste receptor cells, the researchers also studied the importance of OTOP1 in mice by measuring the activity of the gustatory nerves in response to sour-tasting solutions introduced into the mouth of the mice. For these experiments, they teamed up with leading taste researcher Sue Kinnamon and graduate student Courtney Wilson at the University of Colorado Medical School. As expected, the activity of these nerves was severely reduced in mice with nonfunctional OTOP1, showing that the ability of the mice to sense acidic solutions — and thus sour tastes — was impaired.
“Our results show that OTOP1 is a bona fide sour taste receptor,” Liman said. “This is the first definitive evidence for a protein that is both necessary and sufficient for sour taste receptor cells to respond to acids and stimulate the nerves to enable sour taste perception.”
Surprisingly, the scientists found that mice with a nonfunctional Otop1 gene could still produce a small response to sour taste stimuli; the sour taste receptor cells still produced a few action potentials and the gustatory nerve produced a small response to very acidic stimuli. They postulate that another signaling mechanism, unrelated to OTOP1, also contributes to sour taste. They also tested the behavior in mice and found that the mice with a nonfunctional Otop1 gene still found acidic stimuli aversive.
“The behavioral response to acidic stimuli that are ingested is complex. You have the taste receptor cells that can detect acids, but you also have the pain system, which responds to low pH,” Liman said.
“Finding the molecular basis for the sour taste sensor takes us one step closer to understanding how different animals and individuals perceive the world.”
Identification of the molecule responsible for taste opens up possibilities for wide application. This information may lead to an understanding of individual differences in food preferences and flavor perception, guide nutrition science and lead to novel approaches to pest control. Professional flavorists and chemists can leverage this information to manipulate flavors to make food or even medications more pleasing to the palate while also making household products containing toxic chemicals less pleasing.
Liman is the lead author of the study, joined by co-authors Bochuan Teng and Yu-Hsiang Tu of USC Dornsife College; Courtney E. Wilson and Sue C. Kinnamon of University of Colorado Medical School; and Narendra R. Joshi of Harvard Medical School.
Funding: This work was supported by NIH grants (#R01 DC013741, #R01 DC012555, #F31 DC015700 and #DP1 AT009497) and the Howard Hughes Medical Institute.
[divider]About this neuroscience research article[/divider]
Source: USC Media Contacts: Gary Polakovic – USC Image Source: The image is credited to USC.
Original Research: Open access “Cellular and Neural Responses to Sour Stimuli Require the Proton Channel Otop1”. Bochuan Teng, Courtney E. Wilson, Yu-Hsiang Tu, Narendra R. Joshi, Sue C. Kinnamon, Emily R. Liman. Current Biology doi:10.1016/j.cub.2019.08.077.
Cellular and Neural Responses to Sour Stimuli Require the Proton Channel Otop1 Highlights • Genetic inactivation of Otop1 in mice eliminates proton currents in taste cells • Otop1-KO mice have severely attenuated cellular responses to acids • Otop1-KO mice have severely attenuated gustatory nerve responses to acids • Otop1 is a sour taste receptor
Summary The sense of taste allows animals to sample chemicals in the environment prior to ingestion. Of the five basic tastes, sour, the taste of acids, had remained among the most mysterious. Acids are detected by type III taste receptor cells (TRCs), located in taste buds across the tongue and palate epithelium. The first step in sour taste transduction is believed to be entry of protons into the cell cytosol, which leads to cytosolic acidification and the generation of action potentials. The proton-selective ion channel Otop1 is expressed in type III TRCs and is a candidate sour receptor. Here, we tested the contribution of Otop1 to taste cell and gustatory nerve responses to acids in mice in which Otop1 was genetically inactivated (Otop1-KO mice). We first show that Otop1 is required for the inward proton current in type III TRCs from different parts of the tongue that are otherwise molecularly heterogeneous. We next show that in type III TRCs from Otop1-KO mice, intracellular pH does not track with extracellular pH and that moderately acidic stimuli do not elicit trains of action potentials, as they do in type III TRCs from wild-type mice. Moreover, gustatory nerve responses in Otop1-KO mice were severely and selectively attenuated for acidic stimuli, including citric acid and HCl. These results establish that the Otop1 proton channel plays a critical role in acid detection in the mouse gustatory system, evidence that it is a bona fide sour taste receptor.
[divider]Feel free to share this Neuroscience News.[/divider]