A Key Step Toward New Treatment for Hereditary Blindness

Summary: Using animal models, researchers demonstrate a potentially effective approach to treating blindness associated with genetic conditions by using mRNA and lipid nanoparticles.

Source: Oregon State University

Oregon State University College of Pharmacy scientists have demonstrated in animal models the possibility of using lipid nanoparticles and messenger RNA, the technology underpinning COVID-19 vaccines, to treat blindness associated with a rare genetic condition.

Researchers developed nanoparticles able to penetrate the neural retina and deliver mRNA to the photoreceptor cells whose proper function makes vision possible.

The study, led by OSU associate professor of pharmaceutical sciences Gaurav Sahay, Oregon State doctoral student Marco Herrera-Barrera and Oregon Health & Science University assistant professor of ophthalmology Renee Ryals, was published today in Science Advances.

The scientists overcame what had been the main limitation of using lipid nanoparticles, or LNPs, to carry genetic material for the purpose of vision therapy—getting them to reach the back of the eye, where the retina is.

Lipids are fatty acids and similar organic compounds including many natural oils and waxes. Nanoparticles are tiny pieces of material ranging in size from one- to 100-billionths of a meter. Messenger RNA delivers instructions to cells for making a particular protein.

With the coronavirus vaccines, the mRNA carried by the LNPs instructs cells to make a harmless piece of the virus’ spike protein, which triggers an immune response from the body. As a therapy for vision impairment resulting from inherited retinal degeneration, or IRD, the mRNA would instruct photoreceptor cells—faulty because of a genetic mutation—to manufacture the proteins needed for sight.

IRD encompasses a group of disorders of varying severity and prevalence that affect one out of every few thousand people worldwide.

The scientists showed, in research involving mice and non-human primates, that LNPs equipped with peptides were able to pass through barriers in the eye and reach the neural retina—where light is turned into electric signals that the brain converts to images.

“We identified a novel set of peptides that can reach the back of the eye,” Sahay said. “We used these peptides to act as zip codes to deliver nanoparticles carrying genetic materials to the intended address within the eye.”

This shows the nanoparticles entering the retina
Nanoparticles entering neural retina. Credit: Gaurav Sahay, OSU College of Pharmacy

“The peptides that we have discovered can be used as targeting ligands directly conjugated to silencing RNAs, small molecules for therapeutics or as imaging probes,” Herrera-Barrera added.

Sahay and Ryals have received a $3.2 million grant from the National Eye Institute to continue studying lipid nanoparticles’ promise in the treatment of hereditary blindness. They will lead research into using LNPs to deliver a gene editing tool that could delete bad genes in the photoreceptor cells and replace them with correctly functioning genes.

The research aims to develop solutions for the limitations associated with the current primary means of delivery for gene editing: a type of virus known as adeno-associated virus, or AAV.

“AAV has limited packaging capacity compared to LNPs and it can prompt an immune system response,” Sahay said. “It also doesn’t do fantastically well in continuing to express the enzymes the editing tool uses as molecular scissors to make cuts in the DNA to be edited. We’re hoping to use what we’ve learned so far about LNPs to develop an improved gene editor delivery system.”

About this nanotech and genetics research news

Author: Press Office
Source: Oregon State University
Contact: Press Office – Oregon State University
Image: The image is credited to OSU

Original Research: Open access.
Peptide-guided lipid nanoparticles deliver mRNA to the neural retina of rodents and nonhuman primates” by Marco Herrera-Barrera et al. Science Advances


Peptide-guided lipid nanoparticles deliver mRNA to the neural retina of rodents and nonhuman primates

Lipid nanoparticle (LNP)–based mRNA delivery holds promise for the treatment of inherited retinal degenerations.

Currently, LNP-mediated mRNA delivery is restricted to the retinal pigment epithelium (RPE) and Müller glia. LNPs must overcome ocular barriers to transfect neuronal cells critical for visual phototransduction, the photoreceptors (PRs).

We used a combinatorial M13 bacteriophage–based heptameric peptide phage display library for the mining of peptide ligands that target PRs.

We identified the most promising peptide candidates resulting from in vivo biopanning. Dye-conjugated peptides showed rapid localization to the PRs. LNPs decorated with the top-performing peptide ligands delivered mRNA to the PRs, RPE, and Müller glia in mice. This distribution translated to the nonhuman primate eye, wherein robust protein expression was observed in the PRs, Müller glia, and RPE.

Overall, we have developed peptide-conjugated LNPs that can enable mRNA delivery to the neural retina, expanding the utility of LNP-mRNA therapies for inherited blindness.

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