Summary: Two studies reveal that scientists have misidentified gut stem cells, impacting research and treatments for 15 years. Researchers identified the true stem cells in a different gut region, which could lead to breakthroughs in regenerative medicine.
This discovery highlights the importance of accurate identification for effective treatments. The findings could improve therapies for intestinal diseases and beyond.
Key Facts:
- The true gut stem cells are located in the isthmus region, not the crypts.
- Misidentification of gut stem cells has potentially hindered regenerative medicine progress.
- The new discovery could improve treatments for intestinal diseases and beyond.
Source: Columbia University
Two independent studies by Columbia scientists suggest that research into the gut’s stem cells over the past 15 years has been marred by a case of mistaken identity: Scientists have been studying the wrong cell.
Both studies were published online today in the journal Cell.
The gut’s stem cells are some of the hardest-working stem cells in the body. They work continuously throughout our lives to replenish the short-lived cells that line our intestines. About every four days, these cells—covering a surface about the size of a tennis court—are completely replaced.
Understanding these workaholic stem cells could help scientists turn on less productive stem cells in other organs to repair hearts, lungs, brains, and more.
The gut’s stem cells were supposedly identified more than 15 years ago in a landmark study.
But using new lineage tracing and computational tools, the Columbia teams, led by Timothy Wang and Kelley Yan, found that these cells are descendants of the gut’s true stem cells. The gut’s true stem cells are found in a different location, produce different proteins, and respond to different signals.
“The new work is controversial and paradigm-shifting but could revitalize the [entire?] field of regenerative medicine,” says Timothy Wang, the Dorothy L. and Daniel H. Silberberg Professor of Medicine.
“We know we’re making a lot of waves in the field, but if we’re going to make progress, we need to identify the true stem cells so we can target these cells for therapies,” says Kelley Yan, the Herbert Irving Assistant Professor of Medicine.
We recently spoke with Yan and Wang about the findings and implications.
Why does the gut need stem cells?
KY What’s relevant to this story is a tissue called the intestinal epithelium. This is a single layer of cells that lines the gut and it’s composed of different types of cells that help digest food, absorb nutrients, and fight microbes.
Most of the cells live for only about four days before being replaced, so stem cells must create replacements.
What’s really remarkable about the intestinal lining is how big it is. If we were to fillet open your intestine and lay it flat, it would cover the surface of a tennis court.
The gut’s stem cells may be the hardest working stem cells in the body.
The gut’s stem cells were supposedly identified in 2007, and the discovery was hailed as a breakthrough in stem cell science. What made you think this was a case of mistaken identity?
TW: For the last 17 years, the intestinal stem cell field has assumed that Lgr5, a protein on the cell’s surface, is a specific marker for intestinal stem cells. In other words, all Lgr5+ cells are assumed to be stem cells, and all stem cells are believed to be Lgr5+. These Lgr5+ cells were located at the very bottom of glands, or crypts, in the intestinal lining.
However, in the last decade, problems with this model began to appear. Deleting the Lgr5+ cells in mice, using a genetic approach, did not seem to bother the intestine very much, and the Lgr5+ stem cells reappeared over the course of a week. In addition, the intestine was able to regenerate after severe injury, such as radiation-induced damage, even though the injury destroyed nearly all Lgr5+ cells.
KY: By their very definition, stem cells are the cells that regenerate tissues, so these findings created a paradox. Many high-profile papers have evoked different mechanisms to explain the paradox: Some suggest that other fully mature intestinal cells can walk backward in developmental time and regain stem cell characteristics. Others suggest there’s a dormant population of stem cells that only works when the lining is damaged.
No one has really examined the idea that maybe the Lgr5+ cells really aren’t truly stem cells, which is the simplest explanation.
How did your labs identify the gut’s real stem cells?
TW: My lab collaborated with the former chair of Columbia’s systems biology department, Andrea Califano, who has developed cutting-edge computational algorithms that can reconstruct the relationships among cells within a tissue. We used single-cell RNA sequencing to characterize all the cells in the crypts, the region of the intestine where the stem cells are known to reside, and then fed that data into the algorithms.
These algorithms revealed the source of “stemness” in the intestine not in the Lgr5+ cellular pool but in another type of cell higher up in the crypts in a region known as the isthmus. After eliminating Lgr5+ cells with radiation or genetic ablation, we confirmed these isthmus cells were the gut’s stem cells and able to regenerate the intestinal lining. We didn’t find any evidence that other, mature cells could turn back time and become stem cells.
KY: We weren’t trying to identify the stem cells as much as we were trying to understand the other cells in the intestine involved in regeneration of the lining. No one has been able to define these other progenitor cells in the intestine.
We identified a population of cells that were proliferative and marked by a protein called FGFBP1. When we asked how these cells were related to Lgr5+ cells, our computational analysis told us that these FGFBP1 cells give rise to all the intestinal cells, including Lgr5+, the opposite of the accepted model.
My graduate student, Claudia Capdevila, then made a mouse that would allow us to determine which cells—Lgr5+ or FGFBP1+—were the true stem cells. In this mouse, every time the FGFBP1 gene is turned on in a cell, the cell would express two different fluorescent proteins, red and blue. The red would turn on immediately and turn off immediately, while the blue came on a little later and lingered for days.
That allowed us to track the cells over time, and it clearly showed that the FGFBP1 cells create the Lgr5+ cells, the opposite of what people currently believe. This technique, called time-resolved fate mapping, has only been used a few times before, and getting it to work was a pretty incredible achievement, I thought.
How will this affect the stem cell field and the search for stem cell therapies?
TW: This case of mistaken identity may explain why regenerative medicine has not lived up to its promise. We’ve been looking at the wrong cells.
Past studies will need to be reinterpreted in light of the stem cells’ new identity, but eventually it may lead to therapies that help the intestine regenerate in people with intestinal diseases and possible transplantation of stem cells in the future.
KY: Ultimately, we hope to identify a universal pathway that underlies how stem cells work, so we can then apply the principles we learn about the gut to other tissues like skin, hair, brain, heart, lung, kidney, liver, etc.
It’s also thought that some cancers arise from stem cells that have gone awry. So, in understanding the identity of the stem cell, we might be able to also develop novel therapeutics that can prevent the development of cancer.
That’s why it’s so critical to understand what cell underlies all of this.
Additional information
“Time-resolved fate mapping identifies the intestinal upper crypt zone as an origin of Lgr5+ crypt base columnar cells,” was published June 6 in Cell.
All authors: Claudia Capdevila, Jonathan Miller, Liang Cheng, Adam Kornberg, Joel J. George, Hyeonjeong Lee, Theo Botella, Christine S. Moon, John W. Murray, Stephanie Lam, Ermanno Malagola, Gary Whelan, Chyuan-Sheng Lin, Arnold Han, Timothy C. Wang, Peter A. Sims, & Kelley S. Yan. The authors (all from Columbia) declare no competing financial interests.
Funding: The study was supported by the U.S. National Institutes of Health (though grants DP2DK128801, R01AG067014, P30CA013696, P30DK132710, U01DK103155, T32DK083256, and T32HL105323), a Burroughs Wellcome Fund Career Award for Medical Scientists, the Irma T. Hirschl Trust, an Irving Scholars Award, the Gerstner Foundation, a Damon Runyon-Rachleff Innovation Award, a NYSTEM predoctoral training grant, and the Berrie Foundation.
“Isthmus progenitor cells contribute to homeostatic cellular turnover and support regeneration following intestinal injury,” was published June 6 in Cell.
All authors (from Columbia unless noted): Ermanno Malagola, Alessandro Vasciaveo, Yosuke Ochiai, Woosook Kim, Biyun Zheng (Columbia and Fujian Medical University, China), Luca Zanella, Alexander L.E. Wang, Moritz Middelhoff (University Hospital Heidelberg), Henrik Nienhüser (University Hospital Heidelberg), Lu Deng (University of Kansas), Feijing Wu, Quin T. Waterbury, Bryana Belin, Jonathan LaBella, Leah B. Zamechek, Melissa H. Wong (Oregon Health & Sciences University), Linheng Li (University of Kansas), Chandan Guha (Albert Einstein College of Medicine), Chia-Wei Cheng, Kelley S. Yan, Andrea Califano (Columbia and Chan Zuckerberg Biohub NY), and Timothy C. Wang.
Funding: This research was funded by the U.S. National Institutes of Health (through grants P30CA013696, P30DK132710, U01DK103155, R35CA210088, R01NK128195, R35CA197745, S10OD012351, S10OD021764, and S10OD032433) and the U.S. Department of Defense (grants W81XWH-465 21-10901 and W81XWH19-1-0337).
Andrea Califano is founder, equity holder, and consultant of DarwinHealth Inc., a company that has licensed from Columbia University some of the algorithms used in this manuscript. Columbia University is also an equity holder in DarwinHealth Inc. U.S. patent number 10,790,040 has been awarded related to this work, assigned to Columbia University with Andrea Califano as an inventor.
About this stem cell and regenerative medicine research news
Author: Helen Garey
Source: Columbia University
Contact: Helen Garey – Columbia University
Image: The image is credited to Neuroscience News
Original Research: Open access.
“Time-resolved fate mapping identifies the intestinal upper crypt zone as an origin of Lgr5+ crypt base columnar cells” by Claudia Capdevila et al. Cell
Open access.
“Isthmus progenitor cells contribute to homeostatic cellular turnover and support regeneration following intestinal injury” by Ermanno Malagola et al. Cell
Abstract
Time-resolved fate mapping identifies the intestinal upper crypt zone as an origin of Lgr5+ crypt base columnar cells
Highlights
- Intestinal epithelial regeneration originates from the upper crypt, not the crypt base
- Fgfbp1 marks upper crypt homeostatic ISCs that regenerate all lineages and Lgr5+ cells
- Fgfbp1+ and Lgr5+ CBC cells exhibit differential responses to niche signals
- FGFBP1 secreted by upper crypt ISCs is essential for intestinal epithelial regeneration
Summary
In the prevailing model, Lgr5+ cells are the only intestinal stem cells (ISCs) that sustain homeostatic epithelial regeneration by upward migration of progeny through elusive upper crypt transit-amplifying (TA) intermediates.
Here, we identify a proliferative upper crypt population marked by Fgfbp1, in the location of putative TA cells, that is transcriptionally distinct from Lgr5+ cells.
Using a kinetic reporter for time-resolved fate mapping and Fgfbp1-CreERT2 lineage tracing, we establish that Fgfbp1+ cells are multi-potent and give rise to Lgr5+ cells, consistent with their ISC function. Fgfbp1+ cells also sustain epithelial regeneration following Lgr5+ cell depletion.
We demonstrate that FGFBP1, produced by the upper crypt cells, is an essential factor for crypt proliferation and epithelial homeostasis.
Our findings support a model in which tissue regeneration originates from upper crypt Fgfbp1+ cells that generate progeny propagating bi-directionally along the crypt-villus axis and serve as a source of Lgr5+ cells in the crypt base.
Abstract
Isthmus progenitor cells contribute to homeostatic cellular turnover and support regeneration following intestinal injury
Highlights
- Unbiased analysis reveals a novel signature of intestinal stemness-associated genes
- The highest stemness potential is found in the intestinal crypt isthmus
- Isthmus progenitors participate in intestinal homeostasis and regeneration
- De-differentiation or reserve ISCs are not the major drivers of intestinal regeneration
Summary
The currently accepted intestinal epithelial cell organization model proposes that Lgr5+ crypt-base columnar (CBC) cells represent the sole intestinal stem cell (ISC) compartment.
However, previous studies have indicated that Lgr5+ cells are dispensable for intestinal regeneration, leading to two major hypotheses: one favoring the presence of a quiescent reserve ISC and the other calling for differentiated cell plasticity. To investigate these possibilities, we studied crypt epithelial cells in an unbiased fashion via high-resolution single-cell profiling.
These studies, combined with in vivo lineage tracing, show that Lgr5 is not a specific ISC marker and that stemness potential exists beyond the crypt base and resides in the isthmus region, where undifferentiated cells participate in intestinal homeostasis and regeneration following irradiation (IR) injury.
Our results provide an alternative model of intestinal epithelial cell organization, suggesting that stemness potential is not restricted to CBC cells, and neither de-differentiation nor reserve ISC are drivers of intestinal regeneration.
