What the brain really thinks about forever chemicals

Summary: Exposure to perfluorooctane sulfonate and perfluorooctanoic acid, so-called “forever chemicals,” results in a decline of both dopamine levels and dopamine neurons in roundworms.

Source: Purdue University

The human-made chemicals that make our kitchen pans stick-free, our athletic wear water-repellent and firefighting chemicals more efficient do their jobs incredibly well, but it’s at the expense of lingering in the body and environment for what is believed to be forever.

These forever chemicals, perfluorooctane sulfonate and perfluorooctanoic acid, more commonly known as PFOS and PFOA, make life easier. But scientists have many questions about these synthetic compounds’ long-term effects. They are being used less by consumers, and companies that once championed them are stepping away. But they still have important military and industrial applications, especially in firefighting foams. Even if production completely stopped, the world will still live with them forever.

“These chemicals are detectable in the blood of almost every human. They are often found in other species, as well, including polar bear and whale brains,” said Jason Cannon, an associate professor of toxicology at Purdue University. “The carbon-fluorine bond is one of the strongest in nature, and it is difficult to break down, especially when there is more than one fluorine atom on the carbon atom. Most chemicals that we consume or absorb will leave the body in a different chemical form. To the best of our knowledge, these chemicals are not very well metabolized so they bioaccumulate.”

These forever chemicals are something that Cannon refers to as a major public health issue as adverse health effects have been shown to affect hormones and other aspects of the endocrine system. Both PFOS and PFOA are per- and polyfluoroalkyl substances, more commonly known as PFAS, and are potentially neurotoxic. But to what extent is unknown, especially for the years and decades they may accumulate in the brain.

“And which regions they accumulate within the brain is unknown,” said Cannon, who studies dietary toxins and neurological disease, including gene-environment interactions in Parkinson’s disease.

Cannon’s recent study published in Toxicological Sciences determined that PFOS is selectively neurotoxic and relevant to specific neurological diseases. In this study, roundworms were exposed to PFOS and PFAS, and it was shown that their dopamine levels and dopamine neurons were highly sensitive and declined. In mammals, dopamine is key to movement, reward and addiction.

“As a Parkinson’s disease lab, seeing dopamine level depletions is a hallmark feature of the disease, but starting here, we need to be careful of our interpretation,” Cannon said. “Beyond Parkinson’s disease, there are a number of dopamine regions in the brain that could be affected and imply neurodegenerative issues or mental health issues. More research is needed.”

Cannon’s earlier research, published in Toxicology and Applied Pharmacology, took a step toward looking at how dopaminergic function may be affected. This study found that PFOS exposure led to selective decreases of dopamine levels in Northern leopard frogs, a sentinel species. Northern leopard frog larvae were used for testing because their brains feature certain chemical bio features that are more similar to those in humans than those in rodents. This frog species has a breakdown product of dopamine called neuromelanin that binds with toxins.

The three doses evaluated in a laboratory setting reflected high-level amounts from possible military site contaminations. The dopamine decreased significantly in the brains of frogs treated with PFOA at 1,000 parts per billion and PFOS at 100 and 1,000 parts per billion).

“The 1,000 is really pushing it, but the 100, well, there have been some contaminated sites that have been reported to be close to that level,” he said. “This is one of the difficulties in conducting toxicology studies; you want environmentally relevant dose but you also are trying model years of exposure over a short time frame. Certainly, the highest dose is probably pretty weak for environmental relevance, but the two lower doses do bear some environmental relevance. We are conducting studies at even lower doses.”

This shows chemical and a body
Forever chemicals are considered a public health issue as adverse health effects have been shown to affect hormones and other aspects of the endocrine system, and some are potentially neurotoxic. But to what extent is unknown, especially for the years and decades they may accumulate in the brain. Image is credited to Stuart Briers.

These studies on PFAS accumulation in the brain and resultant neurotoxicity were possible thanks to collaborations with labs led by Linda S. Lee, professor of agronomy, and Maria S. Sepúlveda, professor of ecology and natural systems. Cannon also is a member of Purdue’s Institute for Integrative Neuroscience and the Center for the Environment, both of which are in Purdue’s Discovery Park. He also is a member of the Center for Research on Brain, Behavior and NeuroRehabilitation, CEREBBRAL, in Purdue’s College of Health and Human Sciences.

Although many PFOS and PFOAs have been removed, many alternatives with shorter chains are being created.

“It is thought that the shorter chain PFOAs are less toxic,” Cannon said. “But I would say we truly don’t know that. The data is not there. We have all these alternatives in the environment with little to no toxicity testing. In some cases that may be true. They may metabolize and leave the body faster, but from a neurological standpoint the smaller compounds usually penetrate the brain better. So that is something we need to pay attention too.”

Cannon, who is in the School of Health Sciences, is funded by National Institutes of Health and Purdue’s Showalter Research Trust. The first authors on these studies are Shreesh Sammi, a postdoctoral research associate, and Rachel Foguth, a doctoral student, who both work in the Cannon Lab and are actively studying PFAS neurotoxicity.

About this neuroscience research article

Purdue University
Media Contacts:
Amy Patterson Neubert – Purdue University
Image Source:
The image is credited to Stuart Briers.

Original Research: Closed access
“Perfluorooctane Sulfonate (PFOS) Produces Dopaminergic Neuropathology in Caenorhabditis elegans”. Shreesh Raj Sammi, Rachel M. Foguth, Claudia Sofía Nieves, Chloe de Perre, Peter Wipf, Cynthia T. McMurray, Linda S. Lee and Jason R. Cannon.
Toxicological Sciences doi:10.1093/toxsci/kfz191.


Perfluorooctane Sulfonate (PFOS) Produces Dopaminergic Neuropathology in Caenorhabditis elegans

Perfluorooctane sulfonate (PFOS) has been widely utilized in numerous industries. Due to long environmental and biological half-lives, PFOS is a major public health concern. Although the literature suggests that PFOS may induce neurotoxicity, neurotoxic mechanisms, and neuropathology are poorly understood. Thus, the primary goal of this study was to determine if PFOS is selectively neurotoxic and potentially relevant to specific neurological diseases. Nematodes (Caenorhabditis elegans) were exposed to PFOS or related per- and polyfluoroalkyl substances (PFAS) for 72 h and tested for evidence of neuropathology through examination of cholinergic, dopaminergic, gamma-amino butyric acid (GABA)ergic, and serotoninergic neuronal morphologies. Dopaminergic and cholinergic functional analyses were assessed through 1-nonanol and Aldicarb assay. Mechanistic studies assessed total reactive oxygen species, superoxide ions, and mitochondrial content. Finally, therapeutic approaches were utilized to further examine pathogenic mechanisms. Dopaminergic neuropathology occurred at lower exposure levels (25 ppm, approximately 50 µM) than required to produce neuropathology in GABAergic, serotonergic, and cholinergic neurons (100 ppm, approximately 200 µM). Further, PFOS exposure led to dopamine-dependent functional deficits, without altering acetylcholine-dependent paralysis. Mitochondrial content was affected by PFOS at far lower exposure level than required to induce pathology (≥1 ppm, approximately 2 µM). Perfluorooctane sulfonate exposure also enhanced oxidative stress. Further, mutation in mitochondrial superoxide dismutase rendered animals more vulnerable. Neuroprotective approaches such as antioxidants, PFAS-protein dissociation, and targeted (mitochondrial) radical and electron scavenging were neuroprotective, suggesting specific mechanisms of action. In general, other tested PFAS were less neurotoxic. The primary impact is to prompt research into potential adverse outcomes related to PFAS-induced dopaminergic neurotoxicity in humans.

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