Summary: Blast related injuries that do not cause long term brain injury can result in long term retinal injury.
First study to compare neurological and ocular effects of blast injury may open up paths to new interventions, according to a report in The American Journal of Pathology.
Modern military conflict has dramatically increased the number of military personnel and civilians exposed to blast wave pressure. Although traumatic brain injury (TBI) is a well-recognized consequence of extreme blast waves, it is less appreciated that over 80% of combat veterans with TBI also develop visual problems. A new study in The American Journal of Pathology reports that blast exposure that does not cause detectable changes in the brain can result in long-term retinal injury. Researchers identified early indicators of retinal injury and inflammation that may help detect individuals at risk of visual impairment who would then benefit from more timely treatment.
There is a lack of validated biomarkers, as well as limited understanding of the underlying mechanisms of retinal injury due to blast exposure. Currently, diagnosis and measurement of progression of disease rely upon clinical observation, and despite a “normal” ophthalmologic examination, some patients present with chronic visual complaints. Combat veterans show a high percentage of visual field defects, light sensitivity (photophobia), eye movement problems, and decreased contrast sensitivity.
“Our results may explain symptoms of visual dysfunction reported by combat veterans who have experienced TBI,” explained lead investigator Heather West Greenlee, PhD, Director of Graduate Education, Interdepartmental Neuroscience Program, Department of Biomedical Sciences, Iowa State University College of Veterinary Medicine, Ames, IA. “These results are not surprising, since the retina is the most accessible part of the central nervous system; as such, it is particularly vulnerable to injuries similar to those that affect the brain.”
Investigators used a compressed air-driven shock tube system to expose mice to blast wave pressure of 300 kPa (equivalent to 3-times atmospheric pressure) per day for three days. After 30 days, the mice were subjected to a variety of tests to probe for cognition or motor function deficits and subsequently underwent analyses of the retina and brain.
Retinas of blast-pressure-exposed animals displayed several pathological changes, including activation of M?ller glia (principal glial cells of the retina), microglial activation, inflammation, and photoreceptor cell death. Eyes on the same side as the blast showed greater abnormalities than eyes on the other side. Interestingly, researchers also found accumulation of tau, the protein associated with pathologies of the central nervous system including Alzheimer disease, Parkinson disease, and chronic traumatic encephalopathy.
These findings showed the prolonged impact of blast injury on the retina, as well as the vulnerability of particular retinal cell types to blast injury. Despite the effects in the retina, there were no detectable cognitive or motor deficits and no evidence of injury in the striatum or pre-frontal cortex, areas of the brain responsible for motor function. Executive decision making and memory remained unaffected.
“In this model, the retina may serve as an area of the central nervous system that is more vulnerable than the brain and, therefore, may be an effective and more sensitive indicator of low-level injury due to blast-wave pressure,” noted Dr. Greenlee. “We believe that eventually examination of the retina may help to assess the magnitude of exposure and identify individuals who may need or benefit from proactive treatments as they become available.”
Funding: Funding provided by US Army Medical Research and Materiel Command Grant, Stem Cell Research Fund, Departments of Genetics, Development, and Cell Biology, and others.
Source: Eileen Leahy – Elsevier
Image Source: NeuroscienceNews.com image is credited to The American Journal of Pathology.
Original Research: Abstract for “Lasting Retinal Injury in a Mouse Model of Blast-Induced Trauma” by Najiba Mammadova, Shivani Ghaisas, Gary Zenitsky, Donald S. Sakaguchi, Anumantha G. Kanthasamy, Justin J. Greenlee, M. and Heather West Greenlee in American Journal of Pathology. Published online July 9 2017 doi:10.1016/j.ajpath.2017.03.005
Lasting Retinal Injury in a Mouse Model of Blast-Induced Trauma
Traumatic brain injury due to blast exposure is currently the most prevalent of war injuries. Although secondary ocular blast injuries due to flying debris are more common, primary ocular blast exposure resulting from blast wave pressure has been reported among survivors of explosions, but with limited understanding of the resulting retinal pathologies. Using a compressed air-driven shock tube system, adult male and female C57BL/6 mice were exposed to blast wave pressure of 300 kPa (43.5 psi) per day for 3 successive days, and euthanized 30 days after injury. We assessed retinal tissues using immunofluorescence for glial fibrillary acidic protein, microglia-specific proteins Iba1 and CD68, and phosphorylated tau (AT-270 pThr181 and AT-180 pThr231). Primary blast wave pressure resulted in activation of Müller glia, loss of photoreceptor cells, and an increase in phosphorylated tau in retinal neurons and glia. We found that 300-kPa blasts yielded no detectable cognitive or motor deficits, and no neurochemical or biochemical evidence of injury in the striatum or prefrontal cortex, respectively. These changes were detected 30 days after blast exposure, suggesting the possibility of long-lasting retinal injury and neuronal inflammation after primary blast exposure.
“Lasting Retinal Injury in a Mouse Model of Blast-Induced Trauma” by Najiba Mammadova, Shivani Ghaisas, Gary Zenitsky, Donald S. Sakaguchi, Anumantha G. Kanthasamy, Justin J. Greenlee, M. and Heather West Greenlee in American Journal of Pathology. Published online July 9 2017 doi:10.1016/j.ajpath.2017.03.005