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Nanoscale Neuronal Activity Measured for the First Time

A new technique that allows scientists to measure the electrical activity in the communication junctions of the nervous systems has been developed by a researcher at Queen Mary University of London.

The junctions in the central nervous systems that enable the information to flow between neurons, known as synapses, are around 100 times smaller than the width of a human hair (one micrometer and less) and as such are difficult to target let alone measure.

By applying a high-resolution scanning probe microscopy that allows three-dimensional visualisation of the structures, the team were able to measure and record the flow of current in small synaptic terminals for the first time.

This is a 3D illustration of synaptic transmission.

Applying a high-resolution scanning probe microscopy which allowed 3D visualisation of the stuctures, researchers were able to record and measure the flow of currents in small synaptic terminals. This image is a 3d representation of synaptic transmission and is adapted from the Queen Mary, University of London press release.

“We replaced the conventional low-resolution optical system with a high-resolution microscope based on a nanopipette,” said Dr Pavel Novak, a bioengineering specialist from Queen Mary’s School of Engineering and Materials Science.

“The nanopipette hovers above the surface of the sample and scans the structure to reveal its three-dimensional topography. The same nanopipette then attaches to the surface at selected locations on the structure to record electrical activity. By repeating the same procedure for different locations of the neuronal network we can obtain a three-dimensional map of its electrical properties and activity.”

The research, published today in Neuron, opens a new window into the neuronal activity at nanometre scale, and may contribute to the wider effort of understanding the function of the brain represented by the Brain Activity Map Project (BRAIN initiative), which aims to map the function of each individual neuron in the human brain.

Notes about this neuroscience research

The research also involves scientists from University College London and Imperial College London.

Contact: Neha Okhandiar – Queen Mary, University of London
Source: Queen Mary, University of London press release
Image Source: The 3D representation of synaptic transmission image is adapted from the Queen Mary, University of London press release.
Original Research: Abstract for “Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels” by Pavel Novaksend, Julia Gorelik, Umesh Vivekananda, Andrew I. Shevchuk, Yaroslav S. Ermolyuk, Russell J. Bailey, Andrew J. Bushby, Guy W.J. Moss, Dmitri A. Rusakov, David Klenerman, Dimitri M. Kullmann, Kirill E. Volynskisend email, and Yuri E. Korchev in Neuron. Published online September 18 2013 doi:10.1016/j.neuron.2013.07.012

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