Serotonin Neurons Contribute to Fail Safe Mechanism that Ensures Recovery From Interrupted Breathing

Summary: A new study reports acute loss of normal activity in serotonin producing neurons blunts the body’s ability to recover from interrupted breathing. Researchers say this could provide an explanation to why some infants die from SIDs, and could someday help with the development of therapies to prevent sudden infant death.

Source: Harvard.

Scientists trying to identify the roots of sudden infant death syndrome (SIDS), the leading cause of death in U.S. infants between 1 month and 1 year old, have increasingly turned their attention to the neurotransmitter serotonin and the brain cells that produce it.

Studies have linked serotonin-producing neurons to the regulation of breathing, which may go awry in SIDS. In addition, tissue samples from SIDS infants often show abnormalities specifically in those neurons.

Now, a new study in mice suggests that abnormalities in serotonin-producing neurons do not simply accompany a subset of SIDS cases but could actually contribute to those premature deaths.

The findings, published Oct. 23 in eLife, show that an acute loss of normal activity in the serotonin-producing nerve cells blunts the body’s ability to recover from interrupted breathing. The results provide evidence that young animals need properly functioning serotonin neurons to maintain normal cardiorespiratory function.

“If we can determine whether serotonin-producing neurons play an active and necessary role in regulating breathing, heart rate and the recovery response to apneas in young mouse pups, it could provide a plausible biological explanation for at least some SIDS cases,” said Susan Dymecki, professor of genetics at Harvard Medical School and senior author of the study.

“This possible explanation might provide some hope, even if minutely, for the profound grief experienced by families who have lost a child to SIDS, and may one day help researchers prevent SIDS altogether,” she said.

Normally, the brain coordinates the heart and lungs to provide a continuous flow of oxygen into the body and carbon dioxide out.

In conditions such as sleep apnea, when breathing temporarily stops, oxygen levels in cells can fall too low and carbon dioxide levels can rise too high. To restore healthy levels, the brain triggers a series of gasps and raises the heart rate, a process called autoresuscitation.

But heart rate monitor readings in some SIDS infants support the hypothesis that this fail-safe mechanism doesn’t always kick in, and that its failure can lead to SIDS.

Dymecki and colleagues set out to explore the role of serotonin-producing neurons in regulating autoresuscitation in week-old mice, which the researchers estimate are within the corresponding age window that conveys highest risk of SIDS in humans.

The researchers genetically modified the mice so their serotonin neurons would quickly and temporarily be inhibited in response to an injected chemical. Other neurons remained unaffected.

Setting up this inducible neuron perturbation technique in mouse pups and coupling it with the ability to measure respiratory and heart function was the key, said Dymecki.

“Although initially technically challenging, this novel approach allowed for precise brain cell manipulation and real-time measurement of cardiac and respiratory activity,” said Ryan Dosumu-Johnson, a graduate student in the Dymecki lab and first author of the paper.

The researchers then induced apneas in the mice.

serotonin neurons

Serotonin-producing neurons in a mouse brain. NeuroscienceNews.com image is credited to Dymecki lab/Harvard Medical School.

Those with inhibited serotonin-producing neurons had weaker breathing recovery and more instances of sudden death in the face of apneas than mice with a normally functioning serotonin production system.

“These results indicate a vital role for serotonin neurons at an early age after birth,” said Dymecki.

To the researchers’ surprise, the heart rates in mice with inhibited serotonin neurons recovered normally, at least initially, even though their breathing was impaired.

“This uncoupling of the breathing and heart-rate recovery responses was unexpected,” said Dymecki. “It suggests that these two vital physiological responses–heart rate and breathing–could be more separable at the level of brain cells and circuits than previously anticipated, despite their interwoven physiology.”

Although further studies will be needed to uncover whether the same principles hold true in humans, the current findings support the theory that defects in the functioning of serotonin neurons can render infants more vulnerable to dying from apneas and other cardiorespiratory challenges, the authors said.

If replicated in human studies, the new findings could eventually help improve screening tools to identify infants at higher SIDS risk and suggest new strategies for drug development, said Dymecki.

About this neuroscience research article

Additional authors of the paper were postdoctoral researcher YoonJeung Chang of HMS and Andrea Corcoran and Eugene Nattie of the Geisel School of Medicine at Dartmouth.

Funding: This study was funded by National Institutes of Health grant HD036379 as well as a Howard Hughes Medical Institute Gilliam Fellowship for Advanced Study, National Institute of General Medical Sciences award T32GM07753 and the Neuroscience Scholars Program of the Society for Neuroscience.

Source: Ekaterina Pesheva – Harvard
Publisher: Organized by NeuroscienceNews.com.
Image Source: NeuroscienceNews.com image is credited to Dymecki lab/Harvard Medical School.
Original Research: Open access research for “Acute perturbation of Pet1-neuron activity in neonatal mice impairs cardiorespiratory homeostatic recovery” by Ryan T Dosumu-Johnson, Andrea E Cocoran, YoonJeung Chang, Eugene Nattie, and Susan M Dymecki in eLife. Published October 23 2018.
doi:10.7554/eLife.37857

Cite This NeuroscienceNews.com Article
Harvard”Serotonin Neurons Contribute to Fail Safe Mechanism that Ensures Recovery From Interrupted Breathing.” NeuroscienceNews. NeuroscienceNews, 25 October 2018.
<http://neurosciencenews.com/serotonin-neurons-breathing-10090/>.
Harvard(2018, October 25). Serotonin Neurons Contribute to Fail Safe Mechanism that Ensures Recovery From Interrupted Breathing. NeuroscienceNews. Retrieved October 25, 2018 from http://neurosciencenews.com/serotonin-neurons-breathing-10090/
Harvard”Serotonin Neurons Contribute to Fail Safe Mechanism that Ensures Recovery From Interrupted Breathing.” http://neurosciencenews.com/serotonin-neurons-breathing-10090/ (accessed October 25, 2018).

Abstract

Acute perturbation of Pet1-neuron activity in neonatal mice impairs cardiorespiratory homeostatic recovery

Cardiorespiratory recovery from apneas requires dynamic responses of brainstem circuitry. One implicated component is the raphe system of Pet1-expressing (largely serotonergic) neurons, however their precise requirement neonatally for homeostasis is unclear, yet central toward understanding newborn cardiorespiratory control and dysfunction. Here we show that acute in vivo perturbation of Pet1-neuron activity, via triggering cell-autonomously the synthetic inhibitory receptor hM4Di, resulted in altered baseline cardiorespiratory properties and diminished apnea survival. Respiratory more than heart rate recovery was impaired, uncoupling their normal linear relationship. Disordered gasp recovery from the initial apnea distinguished mice that would go on to die during subsequent apneas. Further, the risk likelihood of apnea-related mortality associated with suppression of Pet1 neurons was higher for animals with baseline elevated ventilatory equivalents for oxygen. These findings establish that Pet1 neurons play an active role in neonatal cardiorespiratory homeostasis and provide mechanistic plausibility for the serotonergic abnormalities associated with SIDS.

Feel free to share this Neuroscience News.
Join our Newsletter
I agree to have my personal information transferred to AWeber for Neuroscience Newsletter ( more information )
Sign up to receive the latest neuroscience headlines and summaries sent to your email daily from NeuroscienceNews.com
We hate spam and only use your email to contact you about newsletters. We do not sell email addresses. You can cancel your subscription any time.
No more articles