Direct Amygdala Stimulation Can Enhance Memory in Humans

Summary: Researchers report in a new PNAS study that direct stimulation of the amygdala can enhance memory of images a day later, if the stimulation is applied immediately after the images are viewed.

Source: Emory Health Sciences.

Direct electrical stimulation of the human amygdala, a region of the brain known to regulate memory and emotional behaviors, can enhance next-day recognition of images when applied immediately after the images are viewed, neuroscientists have found.

The results are scheduled for publication in PNAS.

The findings are the first example of electrical brain stimulation in humans giving a time-specific boost to memory lasting more than a few minutes, the scientists say. Patients’ recognition only increased for stimulated images, and not for control images presented in between the stimulated images. The experiments were conducted at Emory University Hospital in 14 epilepsy patients undergoing intracranial monitoring, an invasive procedure for the diagnosis of seizure origin, during which electrodes are introduced into the brain.

“We were able to tag specific memories to be better remembered later,” says co-first author Cory Inman, PhD, postdoctoral fellow in the Department of Neurosurgery. “One day, this could be incorporated into a device aimed at helping patients with severe memory impairments, like those with traumatic brain injuries or mild cognitive impairment associated with various neurodegenerative diseases. However, right now, this is more of a scientific finding than a therapeutic one.”

“We see this as a platform for the further study of memory enhancement,” says senior author Jon T. Willie, MD, PhD, assistant professor of neurosurgery and neurology at Emory University School of Medicine. “The time specificity enables a lot of other experiments, since we know that there’s not a carry-over effect from one image to the next.”

Deep brain stimulation (DBS), with current delivered continuously by an implanted device, is an established clinical method for the treatment of movement disorders such as Parkinson’s disease, and is being tested for psychiatric conditions such as depression. In contrast with DBS’s invasiveness, researchers elsewhere have experimented with non-invasive electrical stimulation as an approach for enhancing memory or cognition, with several rounds of stimulation applied while learning.

“The advantage of DBS is that it can selectively modulate a specific brain circuit without broad off-target effects,” says Willie, who performed surgeries on patients in the study.

The amygdala’s key roles in emotional responses and fear-associated learning have been well-studied. So the Emory scientists made sure that amygdala stimulation at low levels of current (0.5 milliamps) did not result in emotional responses, an elevated heart rate, or other signs of arousal. Study participants reported that they did not notice the stimulation at any point in the study.

The researchers avoided direct stimulation of the hippocampus, figuring that would be too close to the machinery of memory itself, like introducing a live wire into a computer’s motherboard.

“We chose the amygdala because of decades of research in rodents, showing that it interacts with several other memory structures in a modulatory role,” says co-first author Joseph Manns, PhD, associate professor of psychology. “We wanted to stimulate its endogenous function, which we think is to signal salience — something standing out — so that specific experiences are remembered in the future.”

Manns and his colleagues had already shown that electrical amygdala stimulation increases rodents’ ability to recognize images later. The human experiments were designed to closely resemble how his lab’s tests with rodents were set up.

Study participants first viewed 160 neutral objects (emotional faces were excluded, for example) and were asked to judge whether the objects belonged indoors or outdoors. For half of the images, participants received stimulation for one second after each image disappeared from the screen. They were quizzed on half the stimulated images and half the unstimulated images immediately, and the other half the next day. 40 new images were used as decoys. The effect of stimulation on immediate recognition was not statistically strong. However, the next day, the effects on stimulated images were clear.

“This makes sense because the amygdala is thought to be important for memory consolidation – making sure important events stick over time,” Manns says.

79 percent of the participants (11 out of 14) showed an improvement on overnight memory tests, while the remaining 21 percent showed no improvement or impairment. When compared to no stimulation, the increase in number of images accurately recognized ranged from around 8 percent up to several hundred percent.

Image shows the basolateral amygdala highlighted in the brain.
Illustration of the basolateral amygdala (blue), hippocampus (yellow), and perirhinal cortex (pink) and electrical signals from each region during a recognition test trial. 3-D brain model adapted with permission from AMC Virtual Brain Model. image is credited to Cory Inman, Emory University.

Some of the patients had impaired memory as a result of their epilepsy; the patients in whom a greater effect was seen generally had poorer baseline memory performance. For instance, one patient essentially forgot all of the control images, but maintained good memory for the stimulated images. However, a substantial effect was also observed in people who had an average memory to start with.

“The average was like having a ‘B’-level memory performance move up to an ‘A’,” Willie says.

The researchers were also able to see signs of the previous day’s stimulation, in terms of electrical interactions within the brain, when those images were viewed again. The Emory team is now fine-tuning amygdala stimulation parameters, so that memory enhancement might be optimized. They are beginning to look at other types of memory tests, such as spatial or verbal tasks, as well as tasks that more closely mimic memory for real-world events.

“We want to understand the brain’s endogenous mechanisms for memory modulation better before moving ahead with a device,” Manns says.

About this neuroscience research article

Emory co-authors include neurosurgery instructor Kelly Bijanki, PhD, professor of psychology Stephan Hamann, PhD, associate professor of neurology Daniel Drane, PhD, assistant professor of neurology Rebecca Fasano, MD, and Robert Gross, MD, PhD, an Emory neurosurgeon who performed some of the surgeries. Other co-authors are David Bass, MD, PhD, now at the University of Washington, and Christopher Kovach, PhD, at the University of Iowa.

Funding: The research was supported by grants from the National Institute of Neurological Disorders and Stroke (R01NS088748), the National Institute of Mental Health (R01MH100318, F30MH095491), and the National Center for Advancing Translational Sciences (KL2TR000455).

Source: David Barnstone – Emory Health Sciences
Publisher: Organized by
Image Source: image is credited to Cory Inman, Emory University.
Original Research: Abstract for “Direct electrical stimulation of the amygdala enhances declarative memory in humans” by Cory S. Inman, Joseph R. Manns, Kelly R. Bijanki, David I. Bass, Stephan Hamann, Daniel L. Drane, Rebecca E. Fasano, Christopher K. Kovach, Robert E. Gross, and Jon T. Willie in PNAS. Published online December 18 2017 doi:10.1073/pnas.1714058114

Cite This Article

[cbtabs][cbtab title=”MLA”]Emory Health Sciences “Direct Amygdala Stimulation Can Enhance Memory in Humans.” NeuroscienceNews. NeuroscienceNews, 19 December 2017.
<>.[/cbtab][cbtab title=”APA”]Emory Health Sciences (2017, December 19). Direct Amygdala Stimulation Can Enhance Memory in Humans. NeuroscienceNews. Retrieved December 19, 2017 from[/cbtab][cbtab title=”Chicago”]Emory Health Sciences “Direct Amygdala Stimulation Can Enhance Memory in Humans.” (accessed December 19, 2017).[/cbtab][/cbtabs]


Direct electrical stimulation of the amygdala enhances declarative memory in humans

Emotional events are often remembered better than neutral events, a benefit that many studies have hypothesized to depend on the amygdala’s interactions with memory systems. These studies have indicated that the amygdala can modulate memory-consolidation processes in other brain regions such as the hippocampus and perirhinal cortex. Indeed, rodent studies have demonstrated that direct activation of the amygdala can enhance memory consolidation even during nonemotional events. However, the premise that the amygdala causally enhances declarative memory has not been directly tested in humans. Here we tested whether brief electrical stimulation to the amygdala could enhance declarative memory for specific images of neutral objects without eliciting a subjective emotional response. Fourteen epilepsy patients undergoing monitoring of seizures via intracranial depth electrodes viewed a series of neutral object images, half of which were immediately followed by brief, low-amplitude electrical stimulation to the amygdala. Amygdala stimulation elicited no subjective emotional response but led to reliably improved memory compared with control images when patients were given a recognition-memory test the next day. Neuronal oscillations in the amygdala, hippocampus, and perirhinal cortex during this next-day memory test indicated that a neural correlate of the memory enhancement was increased theta and gamma oscillatory interactions between these regions, consistent with the idea that the amygdala prioritizes consolidation by engaging other memory regions. These results show that the amygdala can initiate endogenous memory prioritization processes in the absence of emotional input, addressing a fundamental question and opening a path to future therapies.

“Direct electrical stimulation of the amygdala enhances declarative memory in humans” by Cory S. Inman, Joseph R. Manns, Kelly R. Bijanki, David I. Bass, Stephan Hamann, Daniel L. Drane, Rebecca E. Fasano, Christopher K. Kovach, Robert E. Gross, and Jon T. Willie in PNAS. Published online December 18 2017 doi:10.1073/pnas.1714058114

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