With ability to analyze minuscule amounts of fluid, Rutgers breakthrough could also promote central nervous system and joint research.
Rutgers engineers have developed a breakthrough device that can significantly reduce the cost of sophisticated lab tests for medical disorders and diseases, such as HIV, Lyme disease and syphilis.
The new device uses miniaturized channels and valves to replace “benchtop” assays – tests that require large samples of blood or other fluids and expensive chemicals that lab technicians manually mix in trays of tubes or plastic plates with cup-like depressions.
“The main advantage is cost – these assays are done in labs and clinics everywhere,” said Mehdi Ghodbane, who earned his doctorate in biomedical engineering at Rutgers and now works in biopharmaceutical research and development at GlaxoSmithKline.
Ghodbane and six Rutgers researchers recently published their results in the Royal Society of Chemistry’s journal, Lab on a Chip.
The lab-on-chip device, which employs microfluidics technology, along with making tests more affordable for patients and researchers, opens doors for new research because of its capability to perform complex analyses using 90 percent less sample fluid than needed in conventional tests.
“A great deal of research has been hindered because in many cases one is not able to extract enough fluid,” Ghodbane said.
The Rutgers breakthrough also requires one-tenth of the chemicals used in a conventional multiplex immunoassay, which can cost as much as $1500. Additionally, the device automates much of the skilled labor involved in performing tests.
“The results are as sensitive and accurate as the standard benchtop assay,” said Martin Yarmush, the Paul and Mary Monroe Chair and Distinguished Professor of biomedical engineering at Rutgers and Ghodbane’s adviser.
Until now, animal research on central nervous system disorders, such as spinal cord injury and Parkinson’s disease, has been limited because researchers could not extract sufficient cerebrospinal fluid to perform conventional assays.
“With our technology, researchers will be able to perform large-scale controlled studies with comparable accuracy to conventional assays,” Yarmush said.
The discovery could also lead to more comprehensive research on autoimmune joint diseases such as rheumatoid arthritis through animal studies. As with spinal fluid, the amount of joint fluid, or synovial fluid, researchers are able to collect from lab animals is minuscule.
The Rutgers team has combined several capabilities for the first time in the device they’ve dubbed “ELISA-on-a-chip” (for enzyme-linked immunosorbent assay). A single device analyzes 32 samples at once and can measure widely varying concentrations of as many as six proteins in a sample.
The researchers are exploring the commercial potential of their technology.
Other members of the research team are Elizabeth Stucky, a doctoral student in the Department of Chemical and Biochemical Engineering, and assistant research professor Tim Maguire, associate research professor Rene Schloss, professor David Shreiber and associate professor Jeffrey Zahn, all in the Department of Biomedical Engineering.
Funding: The National Institutes of Health, the National Science Foundation, the New Jersey Commission on Brain Injury Research and Corning, Inc. provided funding for the research.
Source: Carl Blesch – Rutgers
Image Source: The image is credited to Mehdi Ghodbane
Original Research: Abstract for “Development and validation of a microfluidic immunoassay capable of multiplexing parallel samples in microliter volumes” by Mehdi Ghodbane, Elizabeth C. Stucky, Tim J. Maguire, Rene S. Schloss, David I. Shreiber, Jeffrey D. Zahn and Martin L. Yarmush in Lab on a Chip. Published online June 23 2015 doi:10.1039/C5LC00398A
Abstract
Development and validation of a microfluidic immunoassay capable of multiplexing parallel samples in microliter volumes
Immunoassays are widely utilized due to their ability to quantify a vast assortment of biomolecules relevant to biological research and clinical diagnostics. Recently, immunoassay capabilities have been improved by the development of multiplex assays that simultaneously measure multiple analytes in a single sample. However, these assays are hindered by high costs of reagents and relatively large sample requirements. For example, in vitro screening systems currently dedicate individual wells to each time point of interest and this limitation is amplified in screening studies when the investigation of many experimental conditions is necessary; resulting in large volumes for analysis, a correspondingly high cost and a limited temporal experimental design. Microfluidics based immunoassays have been developed in order to overcome these drawbacks. Together, previous studies have demonstrated on-chip assays with either a large dynamic range, high performance sensitivity, and/or the ability to process samples in parallel on a single chip. In this report, we develop a multiplex immunoassay possessing all of these parallel characteristics using commercially available reagents, which allows the analytes of interest to be easily changed. The device presented can measure 6 proteins in 32 samples simultaneously using only 4.2 μL of sample volume. High quality standard curves are generated for all 6 analytes included in the analysis, and spiked samples are quantified throughout the working range of the assay. In addition, we demonstrate a strong correlation (R2 = 0.8999) between in vitro supernatant measurements using our device and those obtained from a bench-top multiplex immunoassay. Finally, we describe cytokine secretion in an in vitro inflammatory hippocampus culture system, establishing proof-of-concept of the ability to use this platform as an in vitro screening tool. The low-volume, multiplexing abilities of the microdevice described in this report could be broadly applied to numerous situations where sample volumes and costs are limiting.
“Development and validation of a microfluidic immunoassay capable of multiplexing parallel samples in microliter volumes” by Mehdi Ghodbane, Elizabeth C. Stucky, Tim J. Maguire, Rene S. Schloss, David I. Shreiber, Jeffrey D. Zahn and Martin L. Yarmush in Lab on a Chip. Published online June 23 2015 doi:10.1039/C5LC00398A
