Research by scientists at Albert Einstein College of Medicine of Yeshiva University may help explain how some cases of autism spectrum disorder (ASD) can result from environmental influences rather than gene mutations. The findings, published online today in PLOS Genetics, shed light on why older mothers are at increased risk for having children with ASD and could pave the way for more research into the role of environment on ASD.
The U.S. Centers for Disease Control and Prevention announced in March that one in 68 U.S. children has an ASD—a 30 percent rise from 1 in 88 two years ago. A significant number of people with an ASD have gene mutations that are responsible for their condition. But a number of studies—particularly those involving identical twins, in which one twin has ASD and the other does not—show that not all ASD cases arise from mutations.
In fact, a major study of more than 14,000 children with ASDs published earlier this month in the Journal of the American Medical Association concluded that gene abnormalities could explain only half the risk for developing ASD. The other half of the risk was attributable to “nongenetic influences,” meaning environmental factors that could include the conditions in the womb or a pregnant woman’s stress level or diet.
Media reports on the causes of ASD have focused on the fact that older fathers (40 and over) are more likely than younger fathers to have children with an ASD, probably because of gene mutations that accumulate over the years in sperm-making cells. Yet older mothers (35 and over) face a similar risk that is entirely independent of their partners’ age. But for older mothers, scientists know very little about why this risk exists. The Einstein researchers looked for genetic as well as environmental influences that might account for older mothers’ increased risk for having children with ASD.
Their study, led by Esther Berko, an M.D./Ph.D. student in the lab of John Greally, M.B., B.Ch., Ph.D., involved 47 children with ASD and 48 typically developing (TD) children of women 35 and over. Unlike other ASD studies, this one included a significant number of minority children (Hispanic and African-American) from the Bronx. Brain cells––the obvious cells to examine for evidence of genetic and environmental differences between ASD and TD children—obviously could not be used. Instead, the researchers realized that if such differences existed, they should also occur in a readily available type of cell: the buccal epithelial cells that line the cheek.
“We hypothesized that whatever influences lead to ASD in children of older women probably are already present in the reproductive cells that produce the embryo or during the very earliest stages of embryonic development—in cells that give rise to both the buccal epithelium and the brain,” said Dr. Greally, the study’s senior author. “This would mean that whatever abnormalities we found in the cheek cells of children with an ASD versus TD children should exist in their brain cells as well.” Dr. Greally is professor of genetics, of medicine and of pediatrics, director of the Center for Epigenomics and the Ruth L. and David S. Gottesman Faculty Scholar for Epigenomics at Einstein and attending physician, pediatrics at The Children’s Hospital at Montefiore.
Small brushes were used to painlessly harvest cheek cells from children with an ASD and TD children living in the Bronx and throughout the U.S., as well as in Chile and Israel. Since the eggs of older mothers are prone to having abnormal numbers of chromosomes, the researchers first analyzed the cells for abnormal chromosome numbers as well as other chromosomal defects that might account for ASD. No such problems were found in the cells of ASD or TD children.
The researchers next examined the children’s cells for evidence of environmental effects. “If environmental influences were exerted during embryonic development, they would encode a “memory” in cells that we can detect as chemical alterations of genes,” said Dr. Greally. “Most of these so-called epigenetic alterations are in the form of methyl groups that chemically bind to DNA. Such methyl groups are vital for controlling gene activity, but changes in methylation patterns can dysregulate cell function by altering gene expression or by silencing genes entirely.”
Dr. Greally and his colleagues carried out several types of genome-wide methylation analyses on the cheek cells of the ASD and TD children, looking for epigenomic differences that would suggest environmental influences at work. (Just as “genome” is defined as the genetic material of an organism, “epigenome” includes the patterns of methyl groups that have attached to an organism’s entire genome.)
This was the largest epigenome-wide association study of ASD to date involving a single pure cell type. Using just one cell type—epithelial cheek cells in this case—helps prevent misleading results that can occur when epigenomic studies combine several different subpopulations of cells. “We were extremely careful in how we conducted this study, to ensure that whatever we found was as scientifically valid as possible,” said Dr. Greally.
The researchers detected two groups of genes that were epigenetically distinctive in children with ASD compared with TD children. Moreover, these genes are known to be expressed in the brain and code for proteins involved in nerve transmission functions previously shown to be impaired in ASD. (Interestingly, these two groups of epigenetically distinctive genes weren’t present in all the cells of children with ASD but only in a subset of them—a phenomenon called mosaicism.) In addition, these two gene groups tended to interact with genes already known to be mutated in individuals with ASD.
“Genes interact with each other to create molecular pathways that carry out important functions,” said Dr. Greally. “Our findings suggest that, at least in some individuals with an ASD, the same pathways in the brain seem to hit by both mutations and epigenetic changes. So the severity of someone’s ASD may depend on whether or not a gene mutation is accompanied by epigenetic alterations to related genes.”
Are environmental influences responsible for the epigenetic changes that dysregulate these genes? “We were able to eliminate some other possible causes of ASD such as chromosomal abnormalities, so our findings are consistent with that notion,” said Dr. Greally. “In the case of older mothers at risk for having children with ASDs, one possible environmental influence might the aging process itself, which could disturb epigenetic patterns in their eggs, but there are other possibilities as well. Although much more work is needed, our study reveals a plausible way that environmental influences—which we know are important in ASD—might be exerting their effects.”
Notes about this Autism research
The study is titled “Mosaic epigenetic dysregulation of ectodermal cells in autism spectrum disorder”. Other Einstein authors of the study were lead author Esther Berko; Masako Suzuki, D.V.M., Ph.D.; Christophe Lemetre; Christine Alaimo; R. Brent Calder; Jill Kirschen, R.N., B.S.N.; Shahina B. Maqbool, Ph.D.; Zeineen Momin; David Reynolds; Karen Ballaban-Gil, M.D.; Natalie Russo, Ph.D.; Maria Valicenti-McDermott, M.D.; Lisa Shulman, M.D.; Edyta Stasiek, Dov Inbar, M.D.; Jessica Tozour, Brett Abrahams, Ph.D.; Joseph Hargitai, Zhengdong Zhang, Ph.D.; Sophie Molholm, Ph.D.; John Foxe, Ph.D.; Robert Marion, M.D.; and Adam Auton, Ph.D. Additional authors were Faygel Beren, Kaylee Kampf and Batya Gounder, Stern College for Women, Yeshiva University (New York, NY); Shenglong Wang, New York University (New York, NY); and Joseph D. Buxbaum, Ph.D., Icahn School of Medicine at Mount Sinai (New York, NY). Funding supporting the project was provided by the Jonas Ehrlich Charitable Foundation and by Autism Speaks. The authors also thank Autism Speaks for its help in recruiting participants for this study.
Contact: Deirdre Branley – Albert Einstein College of Medicine of Yeshiva University Source:Albert Einstein College of Medicine of Yeshiva University press release Image Source: The image is credited to Berko et al./PLOS Genetics and is adapted from the open access research paper. Original Research: Full open access research for “Mosaic epigenetic dysregulation of ectodermal cells in autism spectrum disorder” by Esther R. Berko, Masako Suzuki, Faygel Beren, Christophe Lemetre, Christine M. Alaimo, R. Brent Calder, Karen Ballaban-Gil, Batya Gounder, Kaylee Kampf, Jill Kirschen, Shahina B. Maqbool, Zeineen Momin, David M. Reynolds, Natalie Russo, Lisa Shulman, Edyta Stasiek, Jessica Tozour, Maria Valicenti-McDermott, Shenglong Wang, Brett S. Abrahams, Joseph Hargitai, Dov Inbar, Zhengdong Zhang, Joseph D. Buxbaum, Sophie Molholm, John J. Foxe, Robert W. Marion, Adam Auton, John Greally in PLOS Genetics. Published online May 29 2014 doi:10.1371/journal.pgen.1004402 Abstract for “The Genetic and Environmental Contributions to Autism: Looking Beyond Twins” by Diana E. Schendel, PhD; Therese K. Grønborg, MSc; Erik T. Parner, MSc, PhD in JAMA>. Published online May 8 2014 doi:10.1001/jama.2014.3554
Open Access Neuroscience Abstract
Mosaic Epigenetic Dysregulation of Ectodermal Cells in Autism Spectrum Disorder
DNA mutational events are increasingly being identified in autism spectrum disorder (ASD), but the potential additional role of dysregulation of the epigenome in the pathogenesis of the condition remains unclear. The epigenome is of interest as a possible mediator of environmental effects during development, encoding a cellular memory reflected by altered function of progeny cells. Advanced maternal age (AMA) is associated with an increased risk of having a child with ASD for reasons that are not understood. To explore whether AMA involves covert aneuploidy or epigenetic dysregulation leading to ASD in the offspring, we tested a homogeneous ectodermal cell type from 47 individuals with ASD compared with 48 typically developing (TD) controls born to mothers of ≥35 years, using a quantitative genome-wide DNA methylation assay. We show that DNA methylation patterns are dysregulated in ectodermal cells in these individuals, having accounted for confounding effects due to subject age, sex and ancestral haplotype. We did not find mosaic aneuploidy or copy number variability to occur at differentially-methylated regions in these subjects. Of note, the loci with distinctive DNA methylation were found at genes expressed in the brain and encoding protein products significantly enriched for interactions with those produced by known ASD-causing genes, representing a perturbation by epigenomic dysregulation of the same networks compromised by DNA mutational mechanisms. The results indicate the presence of a mosaic subpopulation of epigenetically-dysregulated, ectodermally-derived cells in subjects with ASD. The epigenetic dysregulation observed in these ASD subjects born to older mothers may be associated with aging parental gametes, environmental influences during embryogenesis or could be the consequence of mutations of the chromatin regulatory genes increasingly implicated in ASD. The results indicate that epigenetic dysregulatory mechanisms may complement and interact with DNA mutations in the pathogenesis of the disorder.
Esther R. Berko, Masako Suzuki, Faygel Beren, Christophe Lemetre, Christine M. Alaimo, R. Brent Calder, Karen Ballaban-Gil, Batya Gounder, Kaylee Kampf, Jill Kirschen, Shahina B. Maqbool, Zeineen Momin, David M. Reynolds, Natalie Russo, Lisa Shulman, Edyta Stasiek, Jessica Tozour, Maria Valicenti-McDermott, Shenglong Wang, Brett S. Abrahams, Joseph Hargitai, Dov Inbar, Zhengdong Zhang, Joseph D. Buxbaum, Sophie Molholm, John J. Foxe, Robert W. Marion, Adam Auton, John Greally in PLOS Genetics, May 29 2014 doi:10.1371/journal.pgen.1004402