Novel Sensors Enable Precise Measurement of Dopamine

Summary: Novel sensors made from modified carbon nanotubes can visualize the release of dopamine from neurons with unprecedented resolution.

Source: RUB

Dopamine is an important signaling molecule for nerve cells. Its concentration could not be precisely determined with both high spatial or temporal resolution until now. A new method has now made this possible.

A research team from Bochum, Göttingen and Duisburg used modified carbon nanotubes that glow brighter in the presence of the messenger substance dopamine. These sensors visualize the release of dopamine from nerve cells with unprecedented resolution.

The researchers headed by Professor Sebastian Kruss from the Physical Chemistry Department at Ruhr-Universität Bochum (RUB) and Dr. James Daniel as well as Professor Nils Brose from the Max Planck Institute for Multidisciplinary Sciences in Göttingen report on this in the journal PNAS from 25 May 2022.

Fluorescence changes in the presence of dopamine

The neurotransmitter dopamine controls the brain’s reward centre, among other things. If this signal transmission no longer functions, it can lead to disorders such as Parkinson’s disease. Moreover, the chemical signals are altered by drugs such as cocaine and play a role in substance abuse disorders.

“However, until now there was no method that could simultaneously visualise the dopamine signals with high spatial and temporal resolution,” explains Sebastian Kruss, head of the Functional Interfaces and Biosystems Group at RUB and a member of the Ruhr Explores Solvation Cluster of Excellence (RESOLV) and the International Graduate School of Neuroscience (IGSN).

This is where the novel sensors come into play. They are based on ultra-thin carbon tubes, about 10,000 times thinner than a human hair. When irradiated with visible light, they glow in the near-infrared range with wavelengths of 1,000 nanometres and more.

“This range of light is not visible to the human eye, but it can penetrate deeper into tissue and thus provide better and sharper images than visible light,” says Kruss. In addition, there are far fewer background signals in this range that can distort the result.

“We have systematically modified this property by binding various short nucleic acid sequences to the carbon nanotubes in such a way that they change their fluorescence when they come into contact with defined molecules,” explains Sebastian Kruss.

This is how his research group has succeeded in turning carbon nanotubes into tiny nanosensors that specifically bind to dopamine and fluoresce more or less strongly depending on the dopamine concentration.

“We immediately realised that such sensors would be interesting for neurobiology,” says Kruss.

Coating healthy nerve cells with a sensor layer

In order to do this, the sensors have to be moved into the vicinity of functioning neuronal networks. Dr. Sofia Elizarova and James Daniel from the Max Planck Institute for Multidisciplinary Sciences in Göttingen developed cell culture conditions for this, in which the nerve cells remain healthy and can be coated with an extremely thin layer of sensors.

This shows the outline of two brains
The neurotransmitter dopamine controls the brain’s reward center, among other things. Image is in the public domain

This allowed the researchers to visualise individual dopamine release events along neuronal structures for the first time and gain insights into the mechanisms of dopamine release.

Kruss, Elizarova and Daniel are confident that the new sensors have enormous potential: “They provide new insights into the plasticity and regulation of dopamine signals,” says Sofia Eizarova.

“In the long term, they could also facilitate progress in the treatment of diseases such as Parkinson’s.” In addition, further sensors are currently being developed with which other signalling molecules can be made visible for example to identify pathogens.

Cooperation partners

The study was conducted by researchers from Physical Chemistry II at Ruhr-Universität Bochum and the Max Planck Institute for Multidisciplinary Sciences in Göttingen, teams from the Institute of Physical Chemistry at Göttingen University, the Centre for Integrative Physiology and Molecular Medicine at Saarland University and the Fraunhofer Institute for Microelectronic Circuits and Systems in Duisburg.

About this neurotech and dopamine research news

Author: Meike Driessen
Source: RUB
Contact: Meike Driessen – RUB
Image: The image is in the public domain

Original Research: Closed access.
A fluorescent nanosensor paint reveals the heterogeneity of dopamine release from neurons at individual release sites” by Sebastian Kruss et al. PNAS


A fluorescent nanosensor paint reveals the heterogeneity of dopamine release from neurons at individual release sites

The neurotransmitter dopamine (DA) controls multiple behaviors and is perturbed in several major brain diseases. DA is released from large populations of specialized structures called axon varicosities. Determining the DA release mechanisms at such varicosities is essential for a detailed understanding of DA biology and pathobiology but has been limited by the low spatial resolution of DA detection methods.

We used a near-infrared fluorescent DA nanosensor paint, adsorbed nanosensors detecting release of dopamine (AndromeDA), to detect DA secretion from cultured murine dopaminergic neurons with high spatial and temporal resolution.

We found that AndromeDA detects discrete DA release events and extracellular DA diffusion and observed that DA release varies across varicosities. To systematically detect DA release hotspots, we developed a machine learning–based analysis tool.

AndromeDA permitted the simultaneous visualization of DA release for up to 100 dopaminergic varicosities, showing that DA release hotspots are heterogeneous and occur at only ∼17% of all varicosities, indicating that many varicosities are functionally silent.

Using AndromeDA, we determined that DA release requires Munc13-type vesicle priming proteins, validating the utility of AndromeDA as a tool to study the molecular and cellular mechanism of DA secretion.

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