Summary: Study reveals the important role astrocytes play in maintaining blood-brain barrier health.
Source: Virginia Tech
Hard skulls help protect our brains from physical injuries.
In addition to a tough outer shell, brains have internal defenses, including a powerful shield called the blood-brain barrier that defends brain cells from substances in the bloodstream that are toxic and dangerous to nerve cells. If the blood-brain barrier is breached, then health problems arise.
Now, in a study with potential impacts on a variety of neurological diseases, Virginia Tech researchers have provided the first experimental evidence from a living organism to show that an abundant, star-shaped brain cell known as an astrocyte is essential for blood-brain barrier health.
The research in today’s online edition of the journal GLIA reassesses traditional claims about the role of astrocytes in the brain and confirms the long-held assumption — although it had been recently disputed — that astrocytes support the blood-brain barrier.
Furthermore, the finding gives scientists a path to understand diseases where frequent blood-brain barrier damage occurs, including traumatic brain injury, stroke, epilepsy, Alzheimer’s disease, and Parkinson’s disease.
“Blood-brain barrier leakage is a problem in the aging brain as well as many different neurological diseases,” said Stefanie Robel, an assistant professor at the Fralin Biomedical Research Institute at VTC and the study’s senior author.
“Without astrocytes, the blood-brain barrier becomes leaky and ineffective, leaving brain tissue vulnerable to a variety of medical conditions. If we know what maintains the barrier in the healthy brain, we will be able to better understand what goes wrong in traumatic brain injury and in Alzheimer’s disease — all health problems with blood-brain barrier damage.”
Generally, researchers suspect astrocytes support the blood-brain barrier by releasing factors helpful to maintaining tight connections between the cells in the barrier.
In the new study, scientists with the Fralin Biomedical Research Institute genetically ablated a small number of astrocytes in adult mice to determine if the cells were necessary for blood-brain barrier health.
The researchers used small, intermediate-sized, and large molecular tracers to evaluate the permeability of the blood-brain barrier. All of the markers passed through the barrier in some areas, while in other areas only small tracers leaked, suggesting that leakages were happening at various sizes.
“We now have great tools to learn about what astrocytes really do in the adult brain,” said Robel, who is also an assistant professor in Virginia Tech’s School of Neuroscience. “It’s possible that those leaks weren’t detected in previous studies.”
In recent years, contradictory studies have emerged in which scientists removed astrocytes in animal models with no indications of harm to the blood-brain barrier.
However, when Virginia Tech scientists used a mouse model to mimic the effect of the removal of astrocytes, varying degrees of permanent blood-brain barrier damage occurred, with the amount of damage possibly in proportion with the numbers of ablated astrocytes. In these scenarios, the scientists determined neighboring astrocytes do not rescue the blood-brain barrier when it is damaged.
The research began after the scientists challenged commonly accepted statements in neuroscience.
“I would read a review article that would say astrocytes maintain the blood-brain barrier in the adult healthy brain, but rarely was it followed by a citation that would show direct evidence of that fact,” said Benjamin Heithoff, the first author of the study and a graduate student who conducts research in the Robel lab at the Fralin Biomedical Research Institute.
“That lack of evidence told me that the field hadn’t quite teased apart this statement,” Heithoff said. “Assumptions can be held for a long time, and it takes studies like ours, with more sensitive tools, to re-evaluate them. Now that we have reinforced this assumption with direct experimental data, we have positioned ourselves to identify how this vital function of astrocytes is impacted in disease and after injury.”
Breakdown of the blood-brain barrier is correlated with neurodegenerative disease. In cases of traumatic brain injury or concussion, the blood-brain barrier breakdown that can occur is associated with higher risk for lifelong consequences, including cognitive decline and permanent motor deficits.
“When people sustain a concussion, we used to consider this a ‘minor injury.’ But our follow-up study shows that the blood-brain barrier leakage persists in areas where astrocytes are not functioning correctly, which suggests there is a long-term, lasting dysfunction in the barrier,” said Heithoff, who is with the Department of Biological Sciences of the College of Science. “Understanding how that problem occurs and how it can be remedied are important public health questions. We have to know what makes this barrier functional in order to develop effective treatments when it becomes dysfunctional.”
Funding: The study was supported by the National Institute of Neurological Disorders and Stroke at the National Institutes of Health.
About this neuroscience research article
Source: Virginia Tech Contacts: John Pastor – Virginia Tech Image Source: The image is credited to Ben Heithoff et al/Virginia Tech.
Astrocytes are necessary for blood–brain barrier maintenance in the adult mouse brain
In the adult brain, multiple cell types are known to produce factors that regulate blood–brain barrier (BBB) properties, including astrocytes. Yet several recent studies disputed a role for mature astrocytes at the BBB. To determine if astrocytes contribute a nonredundant and necessary function in maintaining the adult BBB, we used a mouse model of tamoxifen‐inducible astrocyte ablation. In adult mice, tamoxifen induction caused sparse apoptotic astrocyte cell death within 2 hr. Indicative of BBB damage, leakage of the small molecule Cadaverine, and the large plasma protein fibrinogen into the brain parenchyma indicative of BBB damage was detected as early as astrocyte ablation was present. Vessels within and close to regions of astrocyte loss had lower expression of the tight junction protein zonula occludens‐1 while endothelial glucose transporter 1 expression was undisturbed. Cadaverine leakage persisted for several weeks suggesting a lack of barrier repair. This is consistent with the finding that ablated astrocytes were not replaced. Adjacent astrocytes responded with partial nonproliferative astrogliosis, characterized by morphological changes and delayed phosphorylation of STAT3, which restricted dye leakage to the brain and vessel surface areas lacking coverage by astrocytes 1 month after ablation. In conclusion, astrocytes are necessary to maintain BBB integrity in the adult brain. BBB‐regulating factors secreted by other cell types, such as pericytes, are not sufficient to compensate for astrocyte loss.