Summary: Low-frequency optogenetic stimulation of the visual region of the brain impairs an animal’s ability to make fine sensory discriminations.
Source: Salk Institute
It’s easy to miss something you’re not looking for. In a famous example, people were asked to closely observe two groups of people—one group clad in black, the other in white—pass a ball among themselves. Viewers were asked to count the number of times the ball passed from black to white. Remarkably, most observers did not notice a man in a gorilla suit, walking among the players. This ability of the brain to ignore extraneous visual information is critical to how we work and function, but the processes governing perception and attention are not fully understood. Scientists have long theorized that attention to a particular object can alter perception by amplifying certain neuronal activity and suppressing the activity of other neurons (brain “noise”).
Now, Salk scientists have confirmed this theory by showing how too much background noise from neurons can interrupt focused attention and cause the brain to struggle to perceive objects. The findings, which appeared in eLife on February 22, 2019, could help improve designs for visual prosthetics.
“This study informs us about how information is encoded in the electrical circuits in the brain,” says Salk Professor John Reynolds, senior author of the paper. “When a stimulus appears before us, this activates a population of neurons that are selective for that stimulus. Layered on top of that stimulus-evoked response are large, low-frequency fluctuations in neural activity.
Previous work from Reynolds’ laboratory found that when attention is directed to the stimulus, these low-frequency fluctuations are suppressed. Theoretical models of neural information processing suggested that such fluctuations should impair perception and that attention improves perception by filtering these fluctuations out.
To test this idea directly, the researchers turned to a cutting-edge technology called optogenetics, a technique that can affect the activity of neurons by shining lasers onto light-activated proteins. The team used a low-frequency laser stimulation protocol directed at a visual brain region in animals to create low-frequency response fluctuations—the very neural fluctuations that attention suppresses. They measured the impact of this on the animal’s ability to detect a small change in the orientation of a visual stimulus presented on a computer screen. As predicted by the theory, the added noise impaired perception. Then, they repeated the experiment but using a different laser protocol to induce fluctuations over a high-frequency range that attention does not suppress. Consistent with the theory, this had no impact on perception.
“This is the first time this theoretical idea that increased background noise can hurt perception has been tested,” says first and corresponding author Anirvan Nandy, assistant professor at the Yale University School of Medicine and former Salk researcher. “We’ve confirmed that attention does operate largely by suppressing this coordinated neuron firing activity.”
“This work opens a window into the neural code and will become part of our understanding of the neural mechanisms underlying perception. A deeper understanding of the neural language of perception will be critical in building visual prosthetics,” adds Reynolds, holder of the Fiona and Sanjay Jha Chair in Neuroscience.
Next, the scientists plan to examine the different types of cells that make up the visual circuit in the brain in order to better understand the neurological underpinnings of attention and perception.
Other authors included Jonathan J. Nassi, previously of the Salk Institute, and Monika P. Jadi of Yale University.
Funding: The work was funded by the Brain and Behavior Research Foundation, the National Institutes of Health (grants R01 EY021827, T32 EY020503 and R00EY025026), the National Institutes of Health Blueprint for Neuroscience Research and the Gatsby Charitable Foundation.
John Reynolds – Salk Institute
The image is adapted from the Salk Institute news release.
Original Research: Open access
Anirvan Nandy, Jonathan J Nassi, Monika P Jadi, John Reynolds. “Optogenetically induced low-frequency correlations impair perception”. eLife 2019;8:e35123 doi:10.7554/eLife.35123
Optogenetically induced low-frequency correlations impair perception
Deployment of covert attention to a spatial location can cause large decreases in low-frequency correlated variability among neurons in macaque area V4 whose receptive-fields lie at the attended location. It has been estimated that this reduction accounts for a substantial fraction of the attention-mediated improvement in sensory processing. These estimates depend on assumptions about how population signals are decoded and the conclusion that correlated variability impairs perception, is purely hypothetical. Here we test this proposal directly by optogenetically inducing low-frequency fluctuations, to see if this interferes with performance in an attention-demanding task. We find that low-frequency optical stimulation of neurons in V4 elevates correlations among pairs of neurons and impairs the animal’s ability to make fine sensory discriminations. Stimulation at higher frequencies does not impair performance, despite comparable modulation of neuronal responses. These results support the hypothesis that attention-dependent reductions in correlated variability contribute to improved perception of attended stimuli.