Summary: The brains of patients who died as a result of COVID-19 infection displayed some of the same molecular changes found in the brains of those with Alzheimer’s disease, a new small study found. The findings may explain why some long-term COVID sufferers report memory problems.
Source: Columbia University
A study from researchers at Columbia University Vagelos College of Physicians and Surgeons reports that the brains of a small sample of patients who died of COVID display some of the same molecular changes found in the brains of people with Alzheimer’s disease.
The findings could help explain the memory problems reported by sufferers of “long COVID,” though the researchers caution that the study is small—with data from only 10 patients—and needs to be replicated by others.
The study was published Feb. 3 in Alzheimer’s & Dementia: The Journal of the Alzheimer’s Association.
Early reports of “brain fog” and persistent cardiac symptoms in COVID survivors prompted the Columbia researchers to investigate how certain molecules called ryanodine receptors were affected in this new disease.
Defective ryanodine receptors have been implicated in diverse pathogenic processes, ranging from heart and lung disease to the brain’s response to stress and Alzheimer’s disease, as reported in research led by Andrew Marks, MD, chair of the Department of Physiology & Cellular Biophysics at the Vagelos College of Physicians and Surgeons, who led the new study.
“When the COVID pandemic hit, like everybody else I was interested in being helpful and doing what we could do,” says Marks. “What we found is really I think quite unexpected: Not only did we find defective ryanodine receptors in the hearts and lungs of deceased COVID patients, we also found them in their brains.”
Molecular changes
Inside neurons, defective ryanodine receptors have previously been linked to an increase in phosphorylated tau, a well-known hallmark of Alzheimer’s.
In the new study, the Columbia researchers found high levels of phosphorylated tau in the brains of the COVID patients in addition to defective ryanodine receptors.
Phosphorylated tau was found in areas where tau is typically located in Alzheimer’s patients, as well as in areas where tau is not typically located in Alzheimer’s patients. That suggests that phosphorylated tau in the COVID patients could be a sign of early-stage Alzheimer’s and also contribute to other neurological symptoms observed in COVID-19 patients.
Increased levels of phosphorylated tau in the brain are believed to be linked to memory problems in Alzheimer’s and could be causing similar issues in people with long COVID, Marks says.
Based on the findings, together with additional changes found in the brain, the investigators theorize that the immune response characteristic of severe COVID causes inflammation in the brain, which in turn leads to dysfunctional ryanodine receptors and then increases in phosphorylated tau. No changes in the pathways that lead to the formation of amyloid beta—another hallmark of Alzheimer’s—were found.
Future directions
“One interpretation of these findings is that long COVID could be an atypical form of Alzheimer’s and/or that patients who had severe COVID could be predisposed to developing Alzheimer’s later in life,” says Marks, “but much more research needs to be done before we can make more definitive conclusions.”
If the memory and neurological problems of long COVID can be traced to defective ryanodine receptors, a drug under development by Marks may help. The drug is now in early clinical trials to treat a muscle disease caused by an inherited defect in the ryanodine receptor. The drug was able to fix the ryanodine defect when applied to the COVID patients’ brain tissue.
“My greatest hope is that other laboratories will look into our findings, and if they are validated, generate interest in a clinical trial for long COVID,” says Marks.
More information
The research appears in a paper titled, “Alzheimer’s-like signaling in brains of COVID-19 patients.”
Andrew Marks is professor and chair of the Department of Physiology & Cellular Biophysics and the Clyde’56 and Helen Wu Professor of Molecular Cardiology (in Medicine) at the Vagelos College of Physicians and Surgeons and professor of biomedical engineering at Columbia University’s Fu Foundation School of Engineering and Applied Science.
All authors: Steve Reiken, Leah Sittenfeld, Haikel Dridi, Xiaoping Liu, and Andrew R. Marks (all from Columbia).
Funding: The research was supported by the NIH (grant R01NS114570).
Columbia University and Andrew Marks own stock in ARMGO Pharma Inc., a company developing compounds targeting the ryanodine receptor, and have patents on the compounds. Steven Reiken has consulted for ARMGO Pharma in the past 36 months.
About this neurology and COVID-19 research news
Author: Helen Garey
Source: Columbia University
Contact: Helen Garey – Columbia University
Image: The image is in the public domain
Original Research: Open access.
“Alzheimer’s-like signaling in brains of COVID-19 patients” by Andrew Marks et al. Alzheimer’s and Dementia
Abstract
Alzheimer’s-like signaling in brains of COVID-19 patients
Introduction
The mechanisms that lead to cognitive impairment associated with COVID-19 are not well understood.
Methods
Brain lysates from control and COVID-19 patients were analyzed for oxidative stress and inflammatory signaling pathway markers, and measurements of Alzheimer’s disease (AD)-linked signaling biochemistry. Post-translational modifications of the ryanodine receptor/calcium (Ca2+) release channels (RyR) on the endoplasmic reticuli (ER), known to be linked to AD, were also measured by co-immunoprecipitation/immunoblotting of the brain lysates.
Results
We provide evidence linking SARS-CoV-2 infection to activation of TGF-β signaling and oxidative overload. The neuropathological pathways causing tau hyperphosphorylation typically associated with AD were also shown to be activated in COVID-19 patients. RyR2 in COVID-19 brains demonstrated a “leaky” phenotype, which can promote cognitive and behavioral defects.
Discussion
COVID-19 neuropathology includes AD-like features and leaky RyR2 channels could be a therapeutic target for amelioration of some cognitive defects associated with SARS-CoV-2 infection and long COVID.
