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To improve those drugs and to invent better ones, scientists need to know much more about how the molecule affects brain cells and circuits both in health and amid disease. Credit: Neuroscience News

Decoding Serotonin: A Comprehensive Study on Its Impact on Brain and Behavior

Summary: Researchers conducted a detailed study on serotonin, a key molecule in influencing mood and behavior, tracing its impact from the molecular level to the brain’s overall functioning.

Using the simple animal model of C. elegans, the team was able to map serotonin’s effects across the entire brain, giving a comprehensive view of its behavioral influence. The study identified the functional roles of the worm’s six serotonin receptors, revealing complex interactions between them that likely have direct relevance to psychiatric drugs targeting these receptors.

Findings from this research could be crucial in improving existing psychiatric drugs and developing new ones targeting the serotonin system.

Key Facts:

  1. The researchers at MIT used the simple animal model of C. elegans to map out the effects of serotonin across the entire brain, giving a comprehensive view of its impact on behavior.
  2. The study identified the functional roles of six different serotonin receptors in C. elegans, and found that these receptors interact in complex ways to control behavior, which could have significant implications for the development of psychiatric drugs.
  3. The researchers discovered that different serotonin receptors respond to different patterns of serotonin release in live animals, suggesting that different serotonin receptors are engaged at different times.

Source: Picower Institute for Learning and Memory

Because serotonin is one of the primary chemicals the brain uses to influence mood and behavior, it is also the most common target of psychiatric drugs.

To improve those drugs and to invent better ones, scientists need to know much more about how the molecule affects brain cells and circuits both in health and amid disease.

In a new study, researchers at The Picower Institute for Learning and Memory at MIT working in a simple animal model present a comprehensive accounting of how serotonin affects behavior from the scale of individual molecules all the way to the animal’s whole brain.

“There have been major challenges in rationally developing psychiatric drugs that target the serotonergic system,” said Steve Flavell, associate professor in The Picower Institute and MIT’s Department of Brain and Cognitive Sciences, and senior author of the study in Cell.

“The system is wildly complex. There are many different types of serotonergic neurons with widespread projections throughout the brain and serotonin acts through many different receptors, which are often activated in concert to change the way that neural circuits work.”

These same complexities that scientists face in people are all afoot in the nematode worm C. elegans, but to a more manageably limited degree. C. elegans has only 302 neurons (rather than billions) and only six serotonin receptors (rather than the 14 found in people).

Moreover, all C. elegans neurons and their connections have been mapped out and its cells are accessible for genetic manipulation.

Finally, Flavell’s team has developed imaging technologies that enable them to track and map neural activity across the worm’s brain simultaneously.

For all these reasons, the lab was able to produce a novel study revealing how the far-reaching molecular activity of serotonin changes brain-wide activity and behavior.

“These results provide a global view of how serotonin acts on a diverse set of receptors distributed across a connectome to modulate brain-wide activity and behavior,” the research team wrote in Cell.

The study’s co-lead authors are Picower Institute postdoc Ugur Dag, MIT Brain and Cognitive Sciences graduate student Di Kang, and former research technician Ijeoma Nwabudike, who is now a MD-PhD student at Yale.

Slowing for savoring

Flavell showed in Cell in 2013 that C. elegans uses serotonin to slow down when it reaches a patch of food and traced its source to a neuron called NSM.

In the new study, the team used their many new capabilities developed since then at MIT to examine serotonin’s effects comprehensively.

First, they focused on identifying the functional roles of the worm’s six serotonin receptors.

To do that they created 64 different mutant strains covering the different combinations of knocking out the various receptors. For instance, one strain would have just one receptor knocked out while another strain would have all but that one missing and another would be missing three.

In each of these worms the team stimulated serotonin release from the NSM neuron to prompt slowing behaviors. Analysis of all the resulting data revealed at least two key findings: One was that three receptors primarily drove the slowing behavior. The second was that the other three receptors “interacted” with the receptors that drive slowing and modulated how they function.

These complex interactions between serotonin receptors in the control of behavior is likely to be directly relevant to psychiatric drugs that target these receptors, Flavell said.

The researchers also gained other important insights into serotonin’s actions. One was that different receptors respond to different patterns of serotonin release in live animals.

For example, the SER-4 receptor only responded to sudden increases in serotonin release by the NSM neuron.

But, the MOD-1 receptor responded to continuous “tonic” changes in serotonin release by NSM. This suggests that different serotonin receptors are engaged at different times in the live animal.

Brain-wide mapping

Having teased out the roles of the serotonin receptors in the control of C. elegans behavior, the research team then used their imaging technologies to see how serotonin’s effects worked at a circuit level.

For instance, they fluorescently tagged each receptor gene in each neuron across the brain so that they could see all the specific cells that expressed each receptor, providing a brain-wide map of where the serotonin receptors are located in C. elegans.

About half of the worm’s neurons express serotonin receptors with some neurons expressing as many as five different types.

Finally, the team used their ability to track all neuron activity (based on their calcium fluctuations) and all behaviors to watch how the serotonergic neuron NSM affected other cells’ activity as worms freely explored their surroundings.

About half of the neurons across the worm’s brain changed activity when serotonin was released. Since they knew which exact neurons they were recording from, the research team asked whether knowing which serotonin receptors each cell expressed could predict how they responded to serotonin.

Indeed, knowing which receptors were expressed in each neuron and its input neurons gave strong predictive power of how each neuron was impacted by serotonin.

“We performed brain-wide calcium imaging in freely-moving animals with knowledge of cellular identity during serotonin release, providing, for the first time, a view of how serotonin release is associated with changes in activity across the defined cell types of an animal’s brain,” the researchers concluded.

All these findings shed light on the kinds of complexities and opportunities facing drug developers, Flavell noted. The study’s findings show how the effects of targeting one serotonin receptor could depend on how other receptors or the cell types that express them are functioning.

In particular, the study highlights how the serotonin receptors act in concert to change the activity states of neural circuits.

In addition to Flavell, Dag, Nwabudike and Kang, the paper’s other authors are Matthew Gomes, Jungsoo Kim, Adam Atanas, Eric Bueno, Cassi Estrem, Sarah Pugliese, Ziyu Wang and Emma Towlson.

Funding: Study funders included the National Institutes of Health, the National Science Foundation, the McKnight Foundation, the Alfred P. Sloan Foundation, the Picower Institute and the JPB Foundation.

About this neuroscience research news

Author: David Orenstein
Source: Picower Institute for Learning and Memory
Contact: David Orenstein – Picower Institute for Learning and Memory
Image: The image is credited to Neuroscience News

Original Research: The findings will appear in Cell

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