Summary: Loss of the sense of smell is a common symptom of Parkinson’s disease that often precedes motor deficits. A new study may provide clues as to why the sense of smell diminishes in people with Parkinson’s.Source: University of Auckland.University of Auckland research has found an anatomical link for the loss of the sense of smell in Parkinson’s disease.The research, published in the journal Brain, is the work of academics Associate Professor Maurice Curtis, Dr Victor Dieriks, Dr Sheryl Tan and Sir Richard Faull. All are part of the Centre for Brain Research at the University’s Faculty of Medical and Health Sciences.They worked alongside academics Dr Peter Mombaerts and Dr Bolek Zapiec from the Max Planck Research Unit for Neurogenetics in Frankfurt, Germany. The work in New Zealand was funded by the Neuro Research Charitable Trust, founded by Bernie Crosby who himself has Parkinson’s disease. The work in Germany was funded by the Max Planck Society.Parkinson’s disease is a progressive, degenerative disorder of the brain. Symptoms include tremors, stiffness or rigidity, and slowness of movement. According to Parkinson’s New Zealand, approximately 1 percent of people over the age of 60 have the condition. There is no cure so treatment will normally focus on managing symptoms, typically with medication.Loss of the sense of smell is an often overlooked but remarkably prevalent early symptom of Parkinson’s disease.“A complete loss of smell or a diminished sense of smell often precedes the usual motor symptoms of this neurodegenerative disease by several years, and has a prevalence of 90 per cent in early-stage patients,” Associate Professor Curtis says.The olfactory bulb is a part of the brain that acts as a primary relay station for smell signals from olfactory sensory neurons that detect smells in the nose. These neurons identify smells in the environment. Smell signals are transmitted via axons – the long thread-like part of a nerve cell – of the sensory neurons, which terminate and come together into thousands of structures called glomeruli. The odour-induced signals are processed in the olfactory bulb and relayed to several other parts of the brain including the olfactory cortex.The researchers compared the olfactory bulbs from people with and without Parkinson’s disease and found that the volume taken up by the glomeruli (the functional units of the olfactory bulb) was reduced by more than half in Parkinson’s patients. The researchers discovered that the distribution of the glomeruli was greatly altered. The olfactory bulbs of normal cases had 70 per cent of their glomerular component in the bottom half of the olfactory bulb, but the olfactory bulbs of Parkinson’s disease cases had only 44 per cent in the bottom half.This reduction is consistent with the hypothesis that Parkinson’s disease begins with bacteria, viruses or environmental toxins entering the brain via the nose and affecting first the olfactory bulb, where the neurodegenerative disease is triggered and gradually spreads through other parts of the brain.The New Zealand-based researchers were able to collect olfactory bulbs fit for an in-depth quantitative study. In a globe-spanning project, the researchers processed the post mortem olfactory bulbs, cut thousands of ten-micrometer thin sections throughout its entire length, and stained the sections with fluorescently labeled antibodies. The labeled sections were then scanned in Frankfurt, and the images reconstructed in 3D allowing for quantitative whole-olfactory bulb analyses.“The research has required painstaking attention to detail and collaboration on an international scale. The drive within our international team has been significant to see that this work was done to a high standard and that we used the generously gifted human brain tissue to gain the best results possible,” Associate Professor Curtis says.Glomeruli were identified and profiled using a triple-staining protocol with NCAM, VGLUT2, and βIII-tubulin in pilot experiments. The criterion for glomerular identity was co-labelling with all three antibodies. NeuroscienceNews.com image is credited to the researchers/Brain.As glomeruli of the human olfactory bulb are difficult to count unambiguously, the researchers came up with a new, quantitative parameter: the global glomerular voxel volume. This quantity is the sum of the volume of all the glomeruli in the olfactory bulb. Having defined this new parameter, the researchers compared the values between olfactory bulbs from normal and Parkinson’s disease cases, and found that it was reduced by more than half. Whether the decrease is the result of Parkinson’s patients having fewer or smaller glomeruli, or is due to a combination of these two effects, remains to be seen.The Neurological Foundation of New Zealand Douglas Human Brain Bank at the University of Auckland worked closely with families of patients suffering from neurodegenerative diseases to ensure ethical and effective tissue collection of postmortem brain samples from people with Parkinson’s disease, and people without.This research will be followed up with further work to understand what causes the glomeruli to deteriorate in Parkinson’s disease and to demonstrate what other changes occur in the olfactory bulb in Parkinson’s disease. This work is already under way.[divider]About this neuroscience research article[/divider]See alsoFeaturedNeuroscience·December 6, 2019First of its kind study seeks to answer whether effects of ‘abortion pill’ can be reversedSource: Anna Kellett – University of Auckland Image Source: NeuroscienceNews.com image is credited to the researchers/Brain. Original Research: Full open access research for “A ventral glomerular deficit in Parkinson’s disease revealed by whole olfactory bulb reconstruction” by Bolek Zapiec, Birger V. Dieriks, Sheryl Tan, Richard L. M. Faull, Peter Mombaerts, and Maurice A. Curtis in Brain. Published online September 3 2017 doi:10.1093/brain/awx208[divider]Cite This NeuroscienceNews.com Article[/divider][cbtabs][cbtab title=”MLA”]University of Auckland “Key to Loss of Smell in Parkinson’s Discovered.” NeuroscienceNews. NeuroscienceNews, 20 September 2017. <https://neurosciencenews.com/parkinsons-smell-loss-7528/>.[/cbtab][cbtab title=”APA”]University of Auckland (2017, September 20). Key to Loss of Smell in Parkinson’s Discovered. NeuroscienceNews. Retrieved September 20, 2017 from https://neurosciencenews.com/parkinsons-smell-loss-7528/[/cbtab][cbtab title=”Chicago”]University of Auckland “Key to Loss of Smell in Parkinson’s Discovered.” https://neurosciencenews.com/parkinsons-smell-loss-7528/ (accessed September 20, 2017).[/cbtab][/cbtabs]AbstractA ventral glomerular deficit in Parkinson’s disease revealed by whole olfactory bulb reconstructionOlfactory dysfunction is common in Parkinson’s disease and is an early symptom, but its pathogenesis remains poorly understood. Hindering progress in our mechanistic understanding of olfactory dysfunction in Parkinson’s disease is the paucity of literature about the human olfactory bulb, both from normal and Parkinson’s disease cases. Qualitatively it is well established that the neat arrangement of the glomerular array seen in the mouse olfactory bulb is missing in humans. But rigorous quantitative approaches to describe and compare the thousands of glomeruli in the human olfactory bulb are not available. Here we report a quantitative approach to describe the glomerular component of the human olfactory bulb, and its application to draw statistical comparisons between olfactory bulbs from normal and Parkinson’s disease cases. We subjected horizontal 10 µm sections of olfactory bulbs from six normal and five Parkinson’s disease cases to fluorescence immunohistochemistry with antibodies against vesicular glutamate transporter-2 and neural cell adhesion molecule. We scanned the immunostained sections with a fluorescence slide scanner, segmented the glomeruli, and generated 3D reconstructions of whole olfactory bulbs. We document the occurrence of atypical glomerular morphologies and glomerular-like structures deep in the olfactory bulb, both in normal and Parkinson’s disease cases. We define a novel and objective parameter: the global glomerular voxel volume, which is the total volume of all voxels that are classified immunohistochemically as glomerular. We find that the global glomerular voxel volume in Parkinson’s disease cases is half that of normal cases. The distribution of glomerular voxels along the dorsal-ventral dimension of the olfactory bulb in these series of horizontal sections is significantly altered in Parkinson’s disease cases: whereas most glomerular voxels reside within the ventral half of olfactory bulbs from normal cases, glomerular voxels are more evenly spread among the ventral and dorsal halves of olfactory bulbs from Parkinson’s disease cases. These quantitative whole-olfactory bulb analyses indicate a predominantly ventral deficit in the glomerular component in Parkinson’s disease, consistent with the olfactory vector hypothesis for the pathogenesis of this neurodegenerative disease. The distribution of serine 129-phosphorylated α-synuclein immunoreactive voxels correlates with that of glomerular voxels. The higher the serine 129-phosphorylated α-synuclein load of an olfactory bulb from a Parkinson’s disease case, the lower the global glomerular voxel volume. Our rigorous quantitative approach to the whole olfactory bulb will help understand the anatomy and histology of the normal human olfactory bulb and its pathological alterations in Parkinson’s disease.“A ventral glomerular deficit in Parkinson’s disease revealed by whole olfactory bulb reconstruction” by Bolek Zapiec, Birger V. Dieriks, Sheryl Tan, Richard L. M. Faull, Peter Mombaerts, and Maurice A. Curtis in Brain. Published online September 3 2017 doi:10.1093/brain/awx208[divider]Feel free to share this Neuroscience News.[/divider]Join our Newsletter I agree to have my personal information transferred to AWeber for Neuroscience Newsletter ( more information )Sign up to receive the latest neuroscience headlines and summaries sent to your email daily from NeuroscienceNews.comWe hate spam and only use your email to contact you about newsletters. We do not sell email addresses. You can cancel your subscription any time.