This shows the cells of the retina.
Detail of a cross-section of a retinal organoid. Different tissue structures are made visible with different colours. Credit: Wahle et al. Nature Biotechnology 2023.

High-Resolution Image of the Human Retina Reveals Stunning Details

Summary: Researchers developed a new imaging technique to visualize several dozen proteins in a thin tissue section at high resolution using fluorescence microscopy.

The technique enabled researchers to map the development of human retinal organoids at high temporal and spatial resolution, providing insights into how healthy tissue forms and developing a time series that describes the entire 39-week development of retinal organoids.

The researchers aim to apply this approach to other tissue types, such as the human brain and various tumor tissues, creating an atlas that provides information on the development of human organoids and tissues.

Key Facts:

  1. Researchers are developing an atlas to map out the genes and proteins found in human tissue and organoids to learn more about their development and diseases.
  2. The researchers used a new imaging technique called iterative indirect immunofluorescence imaging (4i) to visualize dozens of proteins in thin tissue sections with high resolution using fluorescence microscopy.
  3. They applied this method to organoids of the human retina and created a time series of images and genetic information that describes the entire 39-week development of retinal organoids.

Source: ETH Zurich

What cell types are found in which human tissue, and where? Which genes are active in the individual cells, and which proteins are found there?

Answers to these questions and more are to be provided by a specialized atlas – in particular how the different tissues form during embryonic development and what causes diseases.

In creating this atlas, researchers aim to map not only tissue directly isolated from humans, but also structures called organoids. These are three-dimensional clumps of tissue that are cultivated in the laboratory and develop in a way similar to human organs, but on a small scale.

“The advantage of organoids is that we can intervene in their development and test active substances on them, which allows us to learn more about healthy tissue as well as diseases,” explains Barbara Treutlein, Professor of Quantitative Developmental Biology at the Department of Biosystems Science and Engineering at ETH Zurich in Basel.

To help produce such an atlas, Treutlein, together with researchers from the Universities of Zurich and Basel, has now developed an approach to gather and compile a great deal of information about organoids and their development. The research team applied this approach to the organoids of the human retina, which they derived from stem cells.

Many proteins visible simultaneously

At the heart of the methods the scientists used for their approach was the 4i technology: iterative indirect immunofluorescence imaging. This new imaging technique can visualize several dozen proteins in a thin tissue section at high resolution using fluorescence microscopy.

The 4i technology was developed a few years ago by Lucas Pelkmans, a professor at the University of Zurich and coauthor of the study that has just been published in the scientific journal Nature Biotechnology.

It is in this study that the researchers applied this method to organoids for the first time.

Typically, researchers use fluorescence microscopy to highlight three proteins in a tissue, each with a different fluorescent dye.

For technical reasons, it is not possible to stain more than five proteins at a time. In 4i technology, three dyes are used, but these are washed from the tissue sample after measurements have been taken, and three new proteins are stained. This step was performed 18 times, by a robot, and the process took a total of 18 days.

Lastly, a computer merges the individual images into a single microscopy image on which 53 different proteins are visible. They provide information on the function of the individual cell types that make up the retina; for example, rods, cones, and ganglion cells.

This shows the cells of the retina.
Detail of a cross-section of a retinal organoid. Different tissue structures are made visible with different colours. Credit: Wahle et al. Nature Biotechnology 2023.

The researchers have supplemented this visual information of retinal proteins with information on which genes are read in the individual cells.

High spatial and temporal resolution

The scientists performed all these analyses on organoids that were of different ages and thus at different stages of development. In this way, they were able to create a time series of images and genetic information that describes the entire 39-week development of retinal organoids.

“We can use this time series to show how the organoid tissue slowly builds up, where which cell types proliferate and when, and where the synapses are located. The processes are comparable to those of retinal formation during embryonic development,” says Gray Camp, a professor at the University of Basel and a senior author of this study.

The researchers published their image information and more findings on retinal development on a publicly accessible website: EyeSee4is.

Further tissue types planned

So far, the scientists have been studying how a healthy retina develops, but in the future, they hope to deliberately disrupt development in retinal organoids with drugs or genetic modifications.

“This will give us new insights into diseases such as retinitis pigmentosa, a hereditary condition that causes the retina’s light-sensitive receptors to gradually degenerate and ultimately leads to blindness,” Camp says.

The researchers want to find out when this process begins and how it can be stopped.

Treutlein and her colleagues are also working on applying the new detailed mapping approach to other tissue types, such as different sections of the human brain and to various tumour tissues. Step by step, this will create an atlas that provides information on the development of human organoids and tissues.

About this neuroscience research news

Author: Press Office
Source: ETH Zurich
Contact: Press Office – ETH Zurich
Image: The image is credited to Wahle et al. Nature Biotechnology 2023

Original Research: Open access.
Multimodal spatiotemporal phenotyping of human retinal organoid development” by Barbara Treutlein et al. Nature Biotechnology


Abstract

Multimodal spatiotemporal phenotyping of human retinal organoid development

Organoids generated from human pluripotent stem cells provide experimental systems to study development and disease, but quantitative measurements across different spatial scales and molecular modalities are lacking.

In this study, we generated multiplexed protein maps over a retinal organoid time course and primary adult human retinal tissue.

We developed a toolkit to visualize progenitor and neuron location, the spatial arrangements of extracellular and subcellular components and global patterning in each organoid and primary tissue. In addition, we generated a single-cell transcriptome and chromatin accessibility timecourse dataset and inferred a gene regulatory network underlying organoid development.

We integrated genomic data with spatially segmented nuclei into a multimodal atlas to explore organoid patterning and retinal ganglion cell (RGC) spatial neighborhoods, highlighting pathways involved in RGC cell death and showing that mosaic genetic perturbations in retinal organoids provide insight into cell fate regulation.

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