Neurons responsible for rapid eye movements/REM during sleep identified

Summary: Nucleus papilion neurons play an important role in eye movement during REM sleep.

Source: University of Bern

REM – Rapid Eye Movement – is not only the name of a successful American rock band, but also and not least a characteristic eye movement in paradoxical sleep, so in the stage with high dream activity. This sleep phase has a peculiarity: Although the muscle tone of the sleeping person completely relaxed, the eyes suddenly move back and forth. The name “paradoxical sleep” is well deserved. Characteristic of these are signs of deep sleep (muscle atony) in connection with a brain activity, which is very similar to those in the waking state, and eye movements. This sleep phase was discovered in the 1950s by French and American researchers and consequently called rapid eye movement sleep (REM sleep), i.e. sleep with rapid eye movements. Why can this strange phenomenon be useful? For 70 years, scientists have been dreaming of getting to the bottom of the mystery. Thanks to the productive cooperation between the universities of Bern and Fribourg, this dream could now come true.

Butterfly wings arranged neurons

For several years, the team led by Franck Girard and Marco Celio at the University of Freiburg has studied neurons under the microscope, which occur in the brain stem and form a structure that is reminiscent of butterfly wings, which is why she was baptized Nucleus papilio. “These neurons are associated with multiple nerve centers, especially those responsible for eye movement, and those involved in sleep control,” explains Franck Girard.

“Therefore, we asked ourselves the following question: may the nucleus papilio neurons play a role in the control of eye movements during sleep?”

This shows a woman sleeping
The researchers from Bern gathered the loop around the nucleus papilio neurons even more closely and were able to demonstrate with the help of optogenetic methods (combined optical and genetic techniques) that their artificial activation causes rapid eye movement, especially during this sleep phase. The image is in the public domain.

Stronger together

To test this hypothesis, the Freiburg researchers turned to the research group headed by Dr. C. Gutiérrez Herrera and Prof. A. Adamantidis at the Department of Neurology at the Inselspital, University Hospital Bern, and Department for BioMedical Research of the University of Bern, who are investigating sleep in mice. “To our surprise, we found that these neurons are particularly active in the phase of paradoxical sleep,” reports Dr. Carolina Gutierrez. The researchers from Bern gathered the loop around the nucleus papilio neurons even more closely and were able to demonstrate with the help of optogenetic methods (combined optical and genetic techniques) that their artificial activation causes rapid eye movement, especially during this sleep phase. Conversely, the inhibition or elimination of these same neurons blocks the movement of the eyes.

After the “how” the “why”!

Now that it is clear that the nucleus papilio neurons play an important role in eye movement during REM sleep, it is important to find out what function this phenomenon has. Is it due to the visual experience of dreams? Does it matter in preserving memories? “Now that we are able to specifically activate the nucleus papilio ‘on demand’ in mice by optogenetic methods, we may be able to find answers to these questions,” says Antoine Adamantidis. The next step, however, will be to confirm the activation of nucleus papilio neurons during REM sleep in humans. The researchers have not yet found the key to their dreams, but they’ve come a long way.

A better understanding of the neural circuits involved in paradoxical sleep is therefore a prerequisite for understanding for instance how these neurons are prone to degenerative changes in diseases such as Parkinson’s.

About this neuroscience research article

Source:
University of Bern
Media Contacts:
Antoine Adamantidis – University of Bern
Image Source:
The image is in the public domain.

Original Research: Open access
“Neurons in the Nucleus papilio contribute to the control of eye movements during REM sleep”. C. Gutierrez Herrera, F. Girard, A. Bilella, T. C. Gent, D. M. Roccaro-Waldmeyer, A. Adamantidis & M. R. Celio.
Nature Communications doi:10.1038/s41467-019-13217-y.

Abstract

Neurons in the Nucleus papilio contribute to the control of eye movements during REM sleep

Rapid eye movements (REM) are characteristic of the eponymous phase of sleep, yet the underlying motor commands remain an enigma. Here, we identified a cluster of Calbindin-D28K-expressing neurons in the Nucleus papilio (NPCalb), located in the dorsal paragigantocellular nucleus, which are active during REM sleep and project to the three contralateral eye-muscle nuclei. The firing of opto-tagged NPCalb neurons is augmented prior to the onset of eye movements during REM sleep. Optogenetic activation of NPCalb neurons triggers eye movements selectively during REM sleep, while their genetic ablation or optogenetic silencing suppresses them. None of these perturbations led to a change in the duration of REM sleep episodes. Our study provides the first evidence for a brainstem premotor command contributing to the control of eye movements selectively during REM sleep in the mammalian brain.

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