Astrocyte Networks in the Mouse Brain Control Spatial Learning and Memory

Summary: In the brains of adult mice, astrocytes coupling contributes to neural functioning in the hippocampus, an area of the brain associated with spatial memory and learning.

Source: University of Zurich

Astrocytes form large networks of interconnected cells in the central nervous system. When these cell-to-cell couplings are disrupted in the brain of adult mice, the animals are no longer able to store spatial information. The astrocytes network is thus essential for spatial learning and memory formation, as neuroscientists of the University of Zurich now show.

In the brain, neurons and astrocytes work together to process information and enable complex behavior and cognitive abilities. Astrocytes have many functions like controlling the blood-brain barrier, providing nutrients to the nervous tissue, and supporting its repair.

An interesting feature of astrocytes is that they form large networks of connected cells. These couplings are made of specific membrane pores that are formed by a group of proteins called connexins. And through these connections, astrocytes can communicate with each other by exchanging various ions and small molecules.

Switching off astrocyte coupling disrupts spatial memory formation

A team of neuroscientists led by Aiman Saab and Bruno Weber at the Institute of Pharmacology and Toxicology of the University of Zurich (UZH), has revealed that in the adult brain of mice astrocyte coupling contributes to neural functioning in the hippocampus, a brain region that is involved in spatial memory formation.

“We found that in adulthood an intact astrocyte network is essential for neural homeostasis, synaptic plasticity and spatial cognitive abilities of this brain region,” says Aiman Saab, last author of the study.

To elucidate the functional relevance of the astrocyte network, the researchers generated a mouse model in which the two key connexins responsible for linking astrocytes together can be selectively inactivated. Once the corresponding genes were turned off, the astrocytes lost their ability to maintain intercellular networks and astrocyte-to-astrocyte coupling was disrupted within a few weeks.

Intact astrocytic network is key for brain functioning of adult mice

Disruption of the astrocyte network altered the excitability of the neurons in the hippocampus and their signal transmission at the synapses. Moreover, the strengthening of these specialized neuronal connections needed to store synaptic information was also compromised. This was accompanied by significant deficits in spatial learning and memory of the animals.

This shows astrocytes
Network of astrocytes in the hippocampus of a mouse under the fluorescence microscope. Credit: Ladina Hösli, University of Zurich

“Astrocyte functions are known to be involved in shaping cognitive abilities. Our study now shows that an intact astrocyte network is critical for spatial memory formation in adult mice,” says Ladina Hösli, first author of the study.

Striking similarities to neurodegenerative diseases and neuropsychiatric disorders

Furthermore, the primary immune cells of the brain are also affected by the loss of astrocyte coupling. The activation of these so-called microglia observed in the mice are similar to changes documented in neurodegenerative diseases such as Alzheimer’s disease and neuropsychiatric disorders like depressions.

“Astrocytes and microglia not only changed their morphology, we also found alterations in specific markers that are characteristic to disease-associated microglia,” says Hösli.

Since normal brain aging is also associated with changes in astrocytic coupling, these glial changes might contribute to the age-related decline in learning and memory.

“Our study shows that in the adult brain the functioning of astrocytic connexins and an intact glial network may be important for the way astrocytes and microglia work together to maintain neural homeostasis,” says Aiman Saab.

In a next step, the researchers aim to understand how microglial functions are altered when astrocyte coupling is perturbed.

About this memory and learning research news

Author: Press Office
Source: University of Zurich
Contact: Press Office – University of Zurich
Image: The image is credited to Ladina Hösli, University of Zurich

Original Research: Open access.
Decoupling astrocytes in adult mice impairs synaptic plasticity and spatial learning” by Ladina Hösli et al. Cell Reports


Decoupling astrocytes in adult mice impairs synaptic plasticity and spatial learning


  • Inducible deletion of Cx30 and Cx43 disrupts astrocytic coupling in adult mice
  • Loss of astrocytic connexins and coupling activate astrocytes and microglia
  • Astroglial networks control hippocampal synaptic transmission and plasticity
  • Disrupting astroglial coupling impairs spatial learning and memory


The mechanisms by which astrocytes modulate neural homeostasis, synaptic plasticity, and memory are still poorly explored. Astrocytes form large intercellular networks by gap junction coupling, mainly composed of two gap junction channel proteins, connexin 30 (Cx30) and connexin 43 (Cx43).

To circumvent developmental perturbations and to test whether astrocytic gap junction coupling is required for hippocampal neural circuit function and behavior, we generate and study inducible, astrocyte-specific Cx30 and Cx43 double knockouts. Surprisingly, disrupting astrocytic coupling in adult mice results in broad activation of astrocytes and microglia, without obvious signs of pathology.

We show that hippocampal CA1 neuron excitability, excitatory synaptic transmission, and long-term potentiation are significantly affected. Moreover, behavioral inspection reveals deficits in sensorimotor performance and a complete lack of spatial learning and memory.

Together, our findings establish that astrocytic connexins and an intact astroglial network in the adult brain are vital for neural homeostasis, plasticity, and spatial cognition.

Join our Newsletter
I agree to have my personal information transferred to AWeber for Neuroscience Newsletter ( more information )
Sign up to receive our recent neuroscience headlines and summaries sent to your email once a day, totally free.
We hate spam and only use your email to contact you about newsletters. You can cancel your subscription any time.
  1. Reminds me of a certain joke I read a while back.
    “A Redneck scientist is on project about cockroach and its behavior.
    He puts cockroach on table n shouts RUN. it starts running.
    He picks it again and cuts out front 2 legs n shouts RUN . Cockroach again runs this time slower.

    He then chop out his 2 middle legs and shouts RUN. Cockroach scrambles it very slowly.

    He then chops remaining 2 legs and shouts RUN. Poor creature don’t move at all.

    He summarises in end – Cockroach becames deaf if you chopout his legs …..!”
    Unless the disruption is controlled and within a specific pathway, one cannot make such conclusions?

Comments are closed.