Summary: A link has been identified between oxidative stress, which has previously been linked to aging and neurodegeneration, and sleep. Oxidative stress activates neurons that control sleep function. The findings could help in the development of treatments for sleep disorders.Source: University of OxfordOxford University researchers have discovered a brain process common to sleep and aging in research that could pave the way for new treatments for insomnia.Writing in the journal Nature, the scientists report how oxidative stress leads to sleep. Oxidative stress is also believed to be a reason why we age and a cause of degenerative diseases.The researchers say the discovery brings us closer to understanding the still-mysterious function of sleep and offers new hope for the treatment of sleep disorders. It may also explain why, as is suspected, chronic lack of sleep shortens life.Professor Gero Miesenböck, Director of Oxford University’s Centre for Neural Circuits and Behaviour, who led the Oxford team, said: ‘It’s no accident that oxygen tanks carry explosion hazard labels: uncontrolled combustion is dangerous. Animals, including humans, face a similar risk when they use the oxygen they breathe to convert food into energy: imperfectly contained combustion leads to “oxidative stress” in the cell. This is believed to be a cause of aging and a culprit for the degenerative diseases that blight our later years. Our new research shows that oxidative stress also activates the neurons that control whether we go to sleep.’The team studied the regulation of sleep in fruit flies – the animal that also provided the first insight into the circadian clock nearly 50 years ago. Each fly has a special set of sleep-control neurons, brain cells that are also found in other animals and believed to exist in people. In previous research, Professor Miesenböck’s team discovered that these sleep-control neurons act like an on-off switch: if the neurons are electrically active, the fly is asleep; when they are silent, the fly is awake.Dr. Seoho Song, a former graduate student in the Miesenböck lab and one of the two lead authors of the study, said: ‘We decided to look for the signals that switch the sleep-control neurons on. We knew from our earlier work that a main difference between sleep and waking is how much electrical current flows through two ion channels, called Shaker and Sandman. During sleep, most of the current goes through Shaker.’Ion channels generate and control the electrical impulses through which brain cells communicate.‘This turned the big, intractable question “Why do we sleep?” into a concrete, solvable problem,’ said Dr. Song. ‘What causes the electrical current to flow through Shaker?’The team found the answer in a component of the Shaker channel itself.The researchers say the discovery brings us closer to understanding the still-mysterious function of sleep and offers new hope for the treatment of sleep disorders. It may also explain why, as is suspected, chronic lack of sleep shortens life. The image is in the public domain.Lead author and postdoctoral fellow in the Miesenböck group, Dr Anissa Kempf, explained: ‘Suspended underneath the electrically conducting portion of Shaker is another part, like the gondola under a hot air balloon. A passenger in the gondola, the small molecule NADPH, flips back and forth between two chemical states – this regulates the Shaker current. The state of NADPH, in turn, reflects the degree of oxidative stress the cell has experienced. Sleeplessness causes oxidative stress, and this drives the chemical conversion.’In a striking demonstration of this mechanism, a flash of light that flipped the chemical state of NADPH put flies to sleep.According to Professor Miesenböck, drugs that change the chemistry of Shaker-bound NADPH in the same way could be a powerful new type of sleeping pill.‘Sleep disturbances are very common,’ he said, ‘and sleeping pills are among the most commonly prescribed drugs. But existing medications carry risks of confusion, forgetfulness and addiction. Targeting the mechanism we have discovered could avoid some of these side effects.’See alsoFeaturedNeuroscienceOpen Neuroscience Articles·April 30, 2020Antibodies from Llamas Could Help in Fight Against COVID-19[divider]About this neuroscience research article[/divider]Source: University of Oxford Media Contacts: Stuart Gillespie – University of Oxford Image Source: The image is in the public domain.Original Research: Closed access “A potassium channel β-subunit couples mitochondrial electron transport to sleep” Anissa Kempf, Seoho M. Song, Clifford B. Talbot & Gero Miesenböck Nature (2019) doi:10.1038/s41586-019-1034-5 AbstractA potassium channel β-subunit couples mitochondrial electron transport to sleepThe essential but enigmatic functions of sleep must be reflected in molecular changes sensed by the brain’s sleep-control systems. In the fruitfly Drosophila, about two dozen sleep-inducing neurons with projections to the dorsal fan-shaped body (dFB) adjust their electrical output to sleep need, via the antagonistic regulation of two potassium conductances: the leak channel Sandman imposes silence during waking, whereas increased A-type currents through Shaker support tonic firing during sleep. Here we show that oxidative byproducts of mitochondrial electron transport regulate the activity of dFB neurons through a nicotinamide adenine dinucleotide phosphate (NADPH) cofactor bound to the oxidoreductase domain of Shaker’s KVβ subunit, Hyperkinetic. Sleep loss elevates mitochondrial reactive oxygen species in dFB neurons, which register this rise by converting Hyperkinetic to the NADP+-bound form. The oxidation of the cofactor slows the inactivation of the A-type current and boosts the frequency of action potentials, thereby promoting sleep. Energy metabolism, oxidative stress, and sleep—three processes implicated independently in lifespan, ageing, and degenerative disease—are thus mechanistically connected. KVβ substrates or inhibitors that alter the ratio of bound NADPH to NADP+ (and hence the record of sleep debt or waking time) represent prototypes of potential sleep-regulatory drugs.[divider]Feel free to share this Neuroscience News.[/divider]Join our Newsletter I agree to have my personal information transferred to AWeber for Neuroscience Newsletter ( more information )Sign up to receive the latest neuroscience headlines and summaries sent to your email daily from NeuroscienceNews.comWe hate spam and only use your email to contact you about newsletters. We do not sell email addresses. You can cancel your subscription any time.