Scientists Uncover Striking Differences Between the Adult and Newborn Mouse Brain

Summary: Study reveals a mismatch between neural activity and blood flow in the brains of newborn mice, shedding light on how the developing brain feeds itself.

Source: Columbia University.

Findings reveal mismatch between neuronal activity and blood flow in the brains of newborn mice, shedding new light on how the growing brain feeds itself.

Columbia scientists have found that spikes in the activity of neurons in young mice do not spur corresponding boosts in blood flow — a discovery that stands in stark contrast to the adult mouse brain. This new study raises questions about how the growing human brain meets its energy needs, as well as how best to track brain development with functional magnetic resonance imaging, or fMRI, which relies on blood-flow changes to map neuronal activity in the brain. The research could also provide critical new insights for improving care for infants.

The findings were published today in the Journal of Neuroscience.

“In the adult brain, neuronal activity triggers a localized boost in blood flow. This relationship between neuronal activity and blood flow has long been assumed to be present from birth, but our findings in mice suggest the opposite: that instead it develops over time,” said Elizabeth Hillman, PhD, a principal investigator at Columbia’s Mortimer B. Zuckerman Mind Brain Behavior Institute, associate professor of biomedical engineering and radiology at Columbia’s Fu Foundation School of Engineering and Applied Science and the paper’s senior author. “Our study further suggests that this process is an essential part of building a healthy brain and could represent an unexplored factor in brain disorders that emerge in early childhood.”

Image shows blood flow in the brains.
The study reveals that neuronal brain activity is not accompanied by a blood flow response in the brains of young mice. Here mice were observed at three ages, 7 days, 13 days and as adults. The left column shows maps of neuronal activity across both sides of the mouse brain during hind-paw stimulation for each age group. The right column shows the blood vessel dilation response to the same stimulus. Patterns of neuronal activity get more complex between the ages of 7 and 10 days, though it’s not until the mouse reaches adulthood that a neuronal activity is accompanied by a match in blood flow. image is credited to Hillman lab/Zuckerman Institute.

Today’s study was motivated by previous fMRI studies in humans that reported vastly different responses in the brains of babies compared to the brains of adults.

“No one knew how to interpret blood-flow responses in the developing brain,” said Mariel Kozberg MD, PhD, a recent Columbia neurobiology graduate in Dr. Hillman’s lab and the paper’s first author. “In this study, we needed to find out what was different between adult and newborn brains. Were the differences in neural activity itself, or did they lie in the relationship between this activity and local blood flow changes?”

To answer this question, Drs. Hillman and Kozberg developed a new imaging technique that simultaneously recorded neuronal activity and blood flow in the brains of mice of different ages (from newborn up to adult), tracking how the brain responded when they stimulated each animal’s hind paw.

“When we started to get data we were amazed by what we could see,” said Dr. Kozberg. First, the team’s innovative imaging methods revealed that, for the youngest mice, stimulating the hind paw caused a strong neuronal response, but this response was localized to one region. Then, as the animals got older, the neuronal response began to spread. By 10 days of age, stimulating the right paw first sparked activity on the left side of the brain before traveling to the right side, corresponding to the development of connections between the two hemispheres.

“We realized we were actually watching cells form connections with each other throughout the brain: the development of neural networks,” added Dr. Hillman, who is also a member of the Kavli Institute for Brain Science at Columbia.

The researchers’ second finding was even more startling. In the youngest mice, neuronal activity did not trigger an increase in blood flow, as occurs in the adult mouse brain. But as the animals matured, and their neural networks became more established, the brain’s blood-flow response gradually got stronger over time until the animal reached adulthood.

“It was like the brain was gradually learning to feed itself,” said Dr. Hillman, who notes that this finding makes a lot of sense. “It is hardly surprising that blood vessels — and the machinery linking them to brain activity — would mature in step with the development of neural activity itself.”

However, these results raised a worrying question. The job of blood vessels is to deliver oxygen-rich blood to the brain. So, can the newborn brain truly function and grow without subsequent increases in blood flow? To find out, Drs. Kozberg and Hillman used another optical imaging technique, called flavoprotein imaging, which measures how the newborn brain used oxygen.

“In the youngest animals, we confirmed that neurons were indeed consuming oxygen, but without a rush of fresh blood, they seemed to run out of fuel,” said Dr. Kozberg. “We further found that the neural activity actually caused localized drops in oxygen levels, known as hypoxias.”

Drs. Hillman and Kozberg propose several explanations for this surprising result. “Newborns make an incredible transition from being inside the womb to breathing air in the delivery room,” noted Dr. Hillman. “To survive those first few hours, the newborn brain must be well prepared to withstand enormous fluctuations in the availability of oxygen.”

Because the hypoxias seen in young mice appear to be part of the normal development process, the authors propose that it may in fact serve an important purpose.

“We know that a lack of oxygen can trigger the growth of blood vessels,” said Dr. Kozberg. “So in this case, neural activity in the newborn brain might actually be guiding blood vessels to grow in the right places.”

Moving forward, the team is preparing to compare their results in mice to the human brain. Dr. Hillman is working with researchers in Columbia’s Department of Psychiatry to analyze hundreds of fMRI scans previously collected from newborns, as well as from children of different ages.

“If we can find the same signatures of neurovascular development in human infants, we could turn fMRI into an even more powerful tool. For example, using it to better understand, detect and track the origins of developmental disorders in the newborn brain,” she said.

Dr. Hillman’s team is also eager to continue studying oxygen metabolism in newborns. Preterm infants exposed to high oxygen levels can suffer from retinopathy, a condition in which blood vessels in the eyes grow incorrectly. She hypothesizes that excessive oxygen could lead to the same disruptions to blood-vessel growth in the brain itself.

Added Dr. Hillman, “If we can learn more about the unique metabolic state of the developing brain, we might be able to improve treatment strategies for premature infants, while also gaining a deeper understanding of normal and abnormal brain development overall.”

About this neuroscience research article

Funding: This research was supported by The National Institutes of Health (1R01NS063226, 1R01NS076628, R21NS053584, T32 GM07367 MSTP, F31 NS084538), the National Science Foundation (CAREER 0954796), the Human Frontier Science Program and the Kavli Foundation.

The authors report no financial or other conflicts of interest.

Source: Anne D. Holden – Columbia University
Image Source: This image is credited to Hillman lab/Zuckerman Institute.
Original Research: Abstract for “Rapid postnatal expansion of neural networks occurs in an environment of altered neurovascular and neurometabolic coupling” by Mariel G. Kozberg, Ying Ma, Mohammed A. Shaik, Sharon H. Kim, and Elizabeth M. C. Hillman in Journal of Neuroscience. Published online June 22 2016 doi:10.1523/JNEUROSCI.2363-15.2016

Cite This Article

[cbtabs][cbtab title=”MLA”]Columbia University. “Scientists Uncover Striking Differences Between the Adult and Newborn Mouse Brain.” NeuroscienceNews. NeuroscienceNews, 22 June 2016.
<>.[/cbtab][cbtab title=”APA”]Columbia University. (2016, June 22). Scientists Uncover Striking Differences Between the Adult and Newborn Mouse Brain. NeuroscienceNews. Retrieved June 22, 2016 from[/cbtab][cbtab title=”Chicago”]Columbia University. “Scientists Uncover Striking Differences Between the Adult and Newborn Mouse Brain.” (accessed June 22, 2016).[/cbtab][/cbtabs]


Rapid postnatal expansion of neural networks occurs in an environment of altered neurovascular and neurometabolic coupling

In the adult brain, increases in neural activity lead to increases in local blood flow. However, many prior measurements of functional hemodynamics in the neonatal brain, including functional magnetic resonance imaging (fMRI) in human infants, have noted altered and even inverted hemodynamic responses to stimuli. Here, we demonstrate that localized neural activity in early postnatal mice does not evoke blood flow increases as in the adult brain, and elucidate the neural and metabolic correlates of these altered functional hemodynamics as a function of developmental age. Using wide-field GCaMP imaging, the development of neural responses to somatosensory stimulus is visualized over the entire bilaterally exposed cortex. Neural responses are observed to progress from tightly localized, unilateral maps to bilateral responses as interhemispheric connectivity becomes established. Simultaneous hemodynamic imaging confirms that spatiotemporally coupled functional hyperemia is not present during these early stages of postnatal brain development, and develops gradually as cortical connectivity is established. Exploring the consequences of this lack of functional hyperemia, measurements of oxidative metabolism via flavoprotein fluorescence suggest that neural activity depletes local oxygen to below baseline levels at early developmental stages. Analysis of hemoglobin oxygenation dynamics at the same age confirms oxygen depletion for both stimulus-evoked and resting-state neural activity. This state of unmet metabolic demand during neural network development poses new questions about the mechanisms of neurovascular development and its role in both normal and abnormal brain development. These results also provide important insights for the interpretation of fMRI studies of the developing brain.

SIGNIFICANCE STATEMENT This work demonstrates that the postnatal development of neuronal connectivity is accompanied by development of the mechanisms that regulate local blood flow in response to neural activity. Novel in vivo imaging reveals that, in the developing mouse brain, strong and localized GCaMP neural responses to stimulus fail to evoke local blood flow increases, leading to a state in which oxygen levels become locally depleted. These results demonstrate that the development of cortical connectivity occurs in an environment of altered energy availability that itself may play a role in shaping normal brain development. These findings have important implications for understanding the pathophysiology of abnormal developmental trajectories, and for the interpretation of functional magnetic resonance imaging data acquired in the developing brain.

“Rapid postnatal expansion of neural networks occurs in an environment of altered neurovascular and neurometabolic coupling” by Mariel G. Kozberg, Ying Ma, Mohammed A. Shaik, Sharon H. Kim, and Elizabeth M. C. Hillman in Journal of Neuroscience. Published online June 22 2016 doi:10.1523/JNEUROSCI.2363-15.2016

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