Summary: Researchers synthesized 3D fuzzy graphene on a nanowire template to create new material for photothermally stimulating cells.

Source: Carnegie Mellon University

A team led by researchers at Carnegie Mellon University has created a new technology that enhances scientists’ ability to communicate with neural cells using light. Tzahi Cohen-Karni, associate professor of biomedical engineering and materials science and engineering, led a team that synthesized three-dimensional fuzzy graphene on a nanowire template to create a superior material for photothermally stimulating cells. NW-templated three-dimensional (3D) fuzzy graphene (NT-3DFG) enables remote optical stimulation without need for genetic modification and uses orders of magnitude less energy than available materials, preventing cellular stress.

Graphene is abundant, cheap, and biocompatible. Cohen-Karni’s lab has been working with graphene for several years, developing a technique of synthesizing the material in 3D topologies that he’s labeled “fuzzy” graphene. By growing two-dimensional (2D) graphene flakes out-of-plane on a silicon nanowire structure, they’re able to create a 3D structure with broadband optical absorption and unparalleled photothermal efficiency.

These properties make it ideal for cellular electrophysiology modulation using light through the optocapacitive effect. The optocapacitive effect alters the cell membrane capacitance due to rapidly applied light pulses. NT-3DFG can be readily made in suspension, allowing the study of cell signaling within and between both 2D cell systems and 3D, like human cell-based organoids.

Systems like these are not only crucial to understanding how cells signal and interact with each other, but also hold great potential for the development of new, therapeutic interventions. Exploration into these opportunities, however, has been limited by the risk of cellular stress that existing optical remote-control technologies present. The use of NT-3DFG eliminates this risk by using significantly less energy, on a scale of 1-2 orders of magnitude less. Its biocompatible surface is easy to modify chemically, making it versatile for use with different cell types and environments. Using NT-3DFG, photothermal stimulation treatments could be developed for motor recruitment to induce muscle activation or could direct tissue development in an organoid system.

“This is an outstanding collaborative work of experts from multiple fields, including neuroscience through Pitt and UChicago, and photonics and materials science through UNC and CMU,” said Cohen-Karni. “The developed technology will allow us to interact with either engineered tissues or with nerve or muscle tissue in vivo. This will allow us to control and affect tissue functionality using light remotely with high precision and low needed energies.”

This shows neurons
Nanowires are able to stimulate neurons from outside the cell membrane. Image is credited to Carnegie Mellon University College of Engineering.

Additional contributions to the project were made by Maysam Chamanzar, assistant professor of electrical and computer engineering. His team’s core expertise in photonics and neurotechnologies assisted in developing the much-needed tools to allow both the characterization of the unique hybrid-nanomaterials, and in stimulating the cells while optically recording their activity.

“The broadband absorption of these 3D nanomaterials enabled us to use light at wavelengths that can penetrate deep into the tissue to remotely excite nerve cells. This method can be used in a whole gamut of applications, from designing non-invasive therapeutics to basic scientific studies,” said Chamanzar.

The team’s findings are significant both for our understanding of cell interactions and the development of therapies that harness the potential of the human body’s own cells. Nanostructures created using NT-3DFG may have a major impact on the future of human biology and medicine.

Other team members involved in the research included Biomedical Engineering Ph.D. student Sahil Rastogi; Materials Science and Engineering Ph.D. students Raghav Garg and Daniel San Roman and master’s student Nicholas Johnson; ECE Ph.D. student Matteo Giuseppe Scopelliti; the University of Chicago’s Francisco Bezanilla and Bernardo Pinto; the University of Pittsburgh’s Jane Hartung and Michael Gold; and the University of North Carolina’s Seokhyoung Kim, Corban Murphey, and James Cahoon.

About this neuroscience research article

Source:
Carnegie Mellon University
Media Contacts:
Dan Carroll – Carnegie Mellon University
Image Source:
The image is credited to Carnegie Mellon University College of Engineering.

Original Research: Open access
“Remote nongenetic optical modulation of neuronal activity using fuzzy graphene”. by Tzahi Cohen-Karni et al.
Transboundary and Emerging Diseases doi:10.1073/pnas.1919921117

Abstract

Remote nongenetic optical modulation of neuronal activity using fuzzy graphene

The ability to modulate cellular electrophysiology is fundamental to the investigation of development, function, and disease. Currently, there is a need for remote, nongenetic, light-induced control of cellular activity in two-dimensional (2D) and three-dimensional (3D) platforms. Here, we report a breakthrough hybrid nanomaterial for remote, nongenetic, photothermal stimulation of 2D and 3D neural cellular systems. We combine one-dimensional (1D) nanowires (NWs) and 2D graphene flakes grown out-of-plane for highly controlled photothermal stimulation at subcellular precision without the need for genetic modification, with laser energies lower than a hundred nanojoules, one to two orders of magnitude lower than Au-, C-, and Si-based nanomaterials. Photothermal stimulation using NW-templated 3D fuzzy graphene (NT-3DFG) is flexible due to its broadband absorption and does not generate cellular stress. Therefore, it serves as a powerful toolset for studies of cell signaling within and between tissues and can enable therapeutic interventions.

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