Researchers Revise Our Knowledge of How the Brain Learns to Fear

Summary: People who express certain autistic traits, such as communication and social difficulties, but without a diagnosis of ASD are at increased risk of suicide, a new study reports.

Source: Cold Spring Harbor Laboratory.

Our brains wire themselves up during development according to a series of remarkable genetic programs that have evolved over millions of years. But so much of our behavior is the product of things we learn only after we emerge from the womb. We aren’t born with instructions to avoid putting our hands on a hot stove. That knowledge comes from experience. Literally and figuratively, it’s easy to get burned, yet we survive and thrive thanks to hard-won knowledge obtained from our experiences.

What happens in the brain when we learn that fire is very hot and can hurt us? It’s the kind of learning that results from the association of a sensory stimulus and the perception of threat. New research in mice appearing today in Nature Neuroscience suggests that the accepted understanding of that critical operation is incorrect in important respects.

A neuroscience team at Cold Spring Harbor Laboratory (CSHL) led by Professor Bo Li studied the relationship between two parts of the brain’s amygdala – an almond-shaped structure that comes in pairs, one situated on each side of the mouse and other vertebrate brains. The amygdalae are centrally involved in learning and memory.

Textbooks indicate that the lateral portion of the amygdala is the place where associations are forged between information provided by the senses and the perception of threat. That linkage is a crucial example of learning – what scientists call aversive learning.

Li’s team performed experiments using several technologies, the results of which surprised them. They suggest that while the lateral amygdala is involved in aversive learning, it is not the seat of the process. The new evidence points to the central amygdala instead. If correct, this could change approaches to modify memory to treat disorders such as anxiety and post-traumatic stress disorder (PTSD).

One series of experiments involved giving a mild foot shock to mice and then imaging the central and lateral parts of the amygdala. A fluorescent protein that registers when cells are active showed that the response to an uncomfortable feeling begins in the central amygdala, then registers in the lateral amygdala. In other experiments, the team blocked the activity of a particular set of neurons in the central amygdala – named for a protein they express called PKC-delta (protein kinase C-delta). When mice with this impairment were given a foot shock, there was greatly attenuated activity in the lateral amygdala. “If the conventional view was correct, you should see normal levels of activity in the lateral amygdala – where the activity supposedly begins. But this wasn’t the case,” says Li.

A final set of experiments had the intriguing aim of giving mice artificial memories – memories of discomfort like that of getting a mild shock. This is done using optogenetics, a technology in which pulses of colored laser light are used to activate specific neurons, in this case the PKC-delta neurons of the central amygdala.

Image shows PKC delta-expressing cells.
A subset of neurons shown here, called PKC delta-expressing cells, located in the brain’s central amygdala, drive aversive learning in mice, neuroscientists from CSHL have demonstrated. image is credited to Li Lab CSHL.

“This causes no pain to the mice, but they don’t like it – it makes them feel uncomfortable, just as getting a mild shock makes them uncomfortable. It’s the kind of stimulation that leads to an aversive memory, the kind you learn from,” explains Li. In effect, the scientists create a memory of a discomfort never actually experienced. The experiment shows that activation of the PKC-delta cells in the central amygdala is indeed at the heart of the aversive learning process.

This was concluded after placing the mice given the optogenetic stimulus into a cage with two chambers. Mice that received the stimulation “remembered” the discomfort and associated it with the place where they experienced it – one of the two chambers. Later, given the freedom to move about in this space, none of the “treated” mice would venture back to the chamber where the stimulation was given. “It appears to be a stable memory,” says Li. “After several days, we saw them continue to avoid the place associated with the discomfort.”

Among the take-away lessons of these experiments is that PKC-delta neurons play an important role in conveying information about unexpected discomfort from the central amygdala to the lateral amygdala. The research suggests that this subset of central amygdala neurons are “fear-on” cells. Li and his team hope that this information will inform ongoing attempts to find new ways to alter fear memories that are based on spurious associations – for example, a combat veteran freezing in fear or experiencing panic at the sound of a backfiring car.

About this neuroscience research article

This research was supported by the NIH, Human Frontier Science Program, BBRF/NARSAD, National Science Foundation of China, China Postdoctoral Science Foundation, Louis Feil Trust, the Stanley Foundation, the simons Foundation and the Wodecroft Foundation.

Source: Peter Tarr – Cold Spring Harbor Laboratory
Publisher: Organized by
Image Source: image is credited to Li Lab CSHL.
Original Research: The study will appear in Nature Neuroscience.

Cite This Article

[cbtabs][cbtab title=”MLA”]Cold Spring Harbor Laboratory “Researchers Revise Our Knowledge of How the Brain Learns to Fear.” NeuroscienceNews. NeuroscienceNews, 23 October 2017.
<>.[/cbtab][cbtab title=”APA”]Cold Spring Harbor Laboratory (2017, October 23). Researchers Revise Our Knowledge of How the Brain Learns to Fear. NeuroscienceNews. Retrieved October 23, 2017 from[/cbtab][cbtab title=”Chicago”]Cold Spring Harbor Laboratory “Researchers Revise Our Knowledge of How the Brain Learns to Fear.” (accessed October 23, 2017).[/cbtab][/cbtabs]

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