Summary: New eye-tracking technology monitors naturalistic eye movements in mice and discovers similarities and differences with human eye movement.
Source: Sainsbury Wellcome Center
In a study published today in Current Biology, Arne Meyer, John O’Keefe and Jasper Poort used a lightweight eye-tracking system composed of miniature video cameras and motion sensors to record head and eye movements in mice without restricting movement or behaviour. Measurements were made while the animals performed naturalistic visual behaviours including social interactions with other mice and visual object tracking. While the eyes in humans typically move together in the same direction, those in mice often moved in opposite directions. Although humans also make eye movement without head movement, for example when reading a book, the study found that mouse eye movements were always linked to head movement.
The researchers identified two types of mouse eye movement coupled to head movement with different functions: ‘head tilt compensation’ and ‘saccade and fixate’ eye movements. ‘Head tilt compensation’ allows mice to maintain a consistent view of the world by compensating for slow changes in head tilt, and results in the two eyes moving in opposite directions, which is typically not observed in humans. ‘Saccade and fixate’ eye movements allow animals to stabilise their view during fast head rotations and shift their gaze in the direction of the head rotation. These ‘saccade and fixate’ movements are similar to those seen in humans and monkeys, which often sample their environment by a sequence of stable images (fixations) and result in the two eyes moving in the same direction. ‘Fixate’ eye movements keep the flow of visual information steady while ‘saccade’ movements allow the animal to select relevant visual information to focus on.
The mouse is an important species to help understand how the human brain functions. First, the organisation and function of the mouse and human brain is similar in many ways, although there are also important differences. Second, scientists can use unique genetic research tools in mice to study brain circuits at a level of detail not possible in other mammals. Third, scientists use genetic tools in mice to model human brain disorders.
The traditional approach to studying vision in humans, monkeys and mice involves restraining head movement. While this facilitates the interpretation of data and allows researchers to use a wider range of experimental measuring methods, it has been unclear whether the results can be generalised to naturalistic behaviours where both head and eyes are free to move. Understanding how mice visually sample their surroundings is also crucial to further close the gap between vision and navigation which has traditionally been studied in freely moving rodents.
This research validates using mice to study important aspects of how humans select visual features that are most important for navigation and decision-making. This visual process is impaired in multiple neurological and neuropsychiatric disorders, including schizophrenia, Alzheimer’s disease and stroke. These impairments are currently difficult to treat, and using mice to model these conditions will allow scientists to study the underlying brain mechanisms to help identify and develop new treatments.
Funding: This research was funded by the Wellcome Trust, the Gatsby Charitable Foundation and the Royal Society.
About this neuroscience research article
Source: Sainsbury Wellcome Center Media Contacts: Hallie Detrick – Sainsbury Wellcome Center Image Source: The image is credited to O’Keefe Lab.
Two Distinct Types of Eye-Head Coupling in Freely Moving Mice
Animals actively interact with their environment to gather sensory information. There is conflicting evidence about how mice use vision to sample their environment. During head restraint, mice make rapid eye movements coupled between the eyes, similar to conjugate saccadic eye movements in humans. However, when mice are free to move their heads, eye movements are more complex and often non-conjugate, with the eyes moving in opposite directions. We combined head and eye tracking in freely moving mice and found both observations are explained by two eye-head coupling types, associated with vestibular mechanisms. The first type comprised non-conjugate eye movements, which compensate for head tilt changes to maintain a similar visual field relative to the horizontal ground plane. The second type of eye movements was conjugate and coupled to head yaw rotation to produce a ‘‘saccade and fixate’’ gaze pattern. During head-initiated saccades, the eyes moved together in the head direction but during subsequent fixation moved in the opposite direction to the head to compensate for head rotation. This saccade and fixate pattern is similar to humans who use eye movements (with or without head movement) to rapidly shift gaze but in mice relies on combined head and eye movements. Both couplings were maintained during social interactions and visually guided object tracking. Even in head-restrained mice, eye movements were invariably associated with attempted head motion. Our results reveal that mice combine head and eye movements to sample their environment and highlight similarities and differences between eye movements in mice and humans.