To better understand the effects of this mechanism on the movements of larval zebrafish, the researchers triggered it experimentally by stimulating the mesencephalic locomotor region. Credit: Neuroscience News
Summary: Researchers uncovered key mechanisms in the brain that control movement initiation. The mesencephalic locomotor region (MLR) in the brain, an ancient and conserved region across various vertebrates, plays a pivotal role in starting movements such as walking, flying, and swimming.
The study utilized the transparency of zebrafish larvae brains to observe and map the neuronal circuits that trigger forward motion. This discovery has immense potential in understanding and addressing motor deficiencies, such as those found in Parkinson’s disease patients.
The mesencephalic locomotor region (MLR) in the brain is vital for initiating a range of movements across various vertebrates, from walking to swimming.
Researchers developed a new method utilizing zebrafish larvae brains’ transparency to track nerve impulse propagation in the brain’s locomotion structures.
The study found that the stimulation intensity of the MLR correlates with the vigor of forward movement in zebrafish, potentially offering insights into gait transitions in both aquatic and terrestrial animals.
Source: Paris Brain Institute
For those fortunate enough to walk normally, wandering is such an expected behavior that we hardly consider that it involves complex, partly involuntary processes.
“Animals move to explore their environment in search of food, interaction with others, or simply out of curiosity. But the perception of danger or a painful stimulus can also activate an automatic flight reflex”, Martin Carbo-Tano, a post-doctoral fellow at Paris Brain Institute, explains.
In both cases, movement initiation relies on the activation of so-called reticulospinal control neurons, which form an intertwined network in the most posterior part of the brain—the brainstem.
These neurons relay nerve signals between the brain and the spinal cord and are essential for motor control of the limbs and trunk and movement coordination.
Upstream of the reticulospinal neurons is the mesencephalic locomotor region (MLR), which is also essential for locomotion since, in animals, its stimulation triggers forward propulsion. It is found in many vertebrates, including monkeys, guinea pigs, cats, salamanders, and even lampreys.
“Because the role of the MLR is conserved in many vertebrate species, we assume that it is an ancient region in their evolution—essential for initiating walking, running, flying, or swimming,” he adds.
“But until now, we didn’t know how this region transmits information to the reticulospinal neurons. This prevented us from gaining a global view of the mechanisms that enable the vertebrae to set themselves in motion and, therefore, from pointing out possible anomalies in this fascinating machinery”.
The pilot station opens its doors
Studying movement initiation is a little tricky: neurons located in the brain stem are not easily accessible and observing their activity in vivo in a moving animal proved difficult. To solve this problem, Martin Carbo-Tano has developed a new approach to stimulate tiny areas in the brain.
Together with Mathilde Lapoix, a Ph.D. student in Claire Wyart’s team at Paris Brain Institute, the researchers took advantage of the transparency of the zebrafish larvae brain to localize the structures involved in locomotion downstream of the MLR and follow the propagation of nerve impulses.
This method, inspired by the work of their collaborator Réjean Dubuc at Montréal University, allowed them to make several remarkable discoveries.
“We observed that neurons in the mesencephalic locomotor region are stimulated when the animal moves spontaneously, but also in response to a visual stimulus. They project through the pons—the central part of the brain stem—and the medulla to activate a subpopulation of reticulospinal neurons called ‘V2a’.
“These neurons control the finer details of movement, such as starting, stopping, and changing direction. In a way, they give steering instructions! Previous work on mice had revealed that reticulospinal neurons control turning; Martin and Mathilde have discovered the control circuit that triggers forward locomotion”, Claire Wyart says.
The midbrain, a concentration of intensity
To better understand the effects of this mechanism on the movements of larval zebrafish, the researchers triggered it experimentally by stimulating the mesencephalic locomotor region. They observed that the duration and vigor of forward movement correlated with the intensity of the stimulation.
“Quadrupeds can adopt different gaits, such as walking, trotting, or galloping. But aquatic animals also mark gait transitions”, Martin Carbo-Tano adds.
“We think that MLR has a role to play in this intensification of movement, which we have observed in zebrafish.”
For the first time, this work made it possible to map the neuronal circuits involved in initiating forward movement—a deficient function in patients with Parkinson’s disease.
This is an essential step in shedding light on the motor control mechanisms upstream of the spinal cord. One day, it may be possible to identify and control all the reticulospinal neurons one by one to model in detail the workings of locomotion and repair those that do not function correctly.