Learning and Memory Formation’s Molecular Basis

Summary: Researchers developed a new platform to explore dendritic translation’s role in memory formation and its implications for intellectual disorders. By employing a novel method named TurboID, researchers uncovered a suite of previously unknown factors involved in memory-related protein synthesis within dendrites, shedding light on the molecular mechanisms that could underlie conditions like Fragile X syndrome.

This study marks a significant advance in understanding how protein synthesis in dendrites contributes to learning and memory, potentially opening new pathways for treating neurodevelopmental disorders. The team’s findings suggest that the localized production of proteins, including newly discovered micropeptides, within dendrites is crucial for memory formation, with implications for diseases characterized by memory impairment.

Key Facts:

  1. The research team identified 1,000 small proteins, or micropeptides, produced in dendrites during memory formation, which were previously unknown.
  2. The study highlighted the role of FMRP, a protein linked to Fragile X syndrome, in binding mRNA within dendrites, suggesting a new avenue for understanding intellectual disabilities.
  3. The development of dendritic-TurboID technology allows for unprecedentedly detailed analysis of protein synthesis in dendrites, offering potential for broad applications in neuroscience research.

Source: Rockefeller University

Less than twenty minutes after finishing this article, your brain will begin to store the information that you’ve just read in a coordinated burst of neuronal activity.

Underpinning this process is a phenomenon known as dendritic translation, which involves an uptick in localized protein production within dendrites, the spiny branches that project off the neuron cell body and receive signals from other neurons at synapses. It’s a process key to memory—and its dysfunction is linked to intellectual disorders.

This shows a brain.
It has since become clear that memories form, in part, because of new protein synthesis made locally in the dendrites of the hippocampus. Credit: Neuroscience News

That makes the inner workings of dendritic translation a “holy grail for understanding memory formation,” says Rockefeller’s Robert B. Darnell, whose team just published a study in Nature Neuroscience describing a new platform capable of identifying the specific regulatory mechanisms that drive dendritic translation.

The team leveraged a method, dubbed TurboID, to discover an entire suite of previously unknown factors in memory formation, revealing now mechanisms that underlie how protein synthesis in dendrites contributes to learning and memory.

The findings may also have implications for intellectual disabilities, such as Fragile X syndrome.

“Technological limitations have long prevented a comprehensive inventory of the activity at the synapse involved in memory formation,” says lead author Ezgi Hacisuleyman, who conducted the research as a postdoctoral researcher in Darnell’s laboratory. She is now an assistant professor at The UF Scripps Institute.

“Our new techniques can accomplish this with extremely high resolution to look at neurons in vitro that are closely mimicking what we see in the brain.”

“Hacisuleyman’s work defines a whole new biochemical pathway which fits with, complements, and vastly expands what we already knew about memory and learning,” adds Darnell, the Robert and Harriet Heilbrunn professor.

A unique way to metabolize RNA

Memory formation centers around the hippocampus, a brain region so central to learning that, when surgeons removed it from people with epilepsy in the 1940s, the patients remembered their childhoods but lost the ability to form new memories.

It has since become clear that memories form, in part, because of new protein synthesis made locally in the dendrites of the hippocampus.

Darnell, a physician-scientist, observed the importance of dendritic translation firsthand while working with patients whose immune systems had attacked the hippocampus.

“I would talk to a patient for 30 minutes, leave the room, walk back in, and it was like they had never seen me before,” he says.

“That’s when I began focusing on why neurons of the hippocampus have their own system for regulating RNA metabolism—a system that no other cell in the body uses.”

That system, it turns out, lies at the heart of how our brains form memories and learn new information, and became a focus for the Darnell lab, culminating in his team’s 2003 development of CLIP, a method that allowed researchers to study the proteins that bind and influence RNA. But limitations remained.

“Many details about how neurons respond to stimuli at the dendrites were still missing,” Hacisuleyman says.

“We needed that information, because that plays a role in determining how neurons function—and where things often go awry in neurologic disease.”

1,000 micropeptides

To get a better idea of the role that changes in dendrites play in learning, Hacisuleyman extended the TurboID platform to works in concert with RNA-sequencing, CLIP, translation and protein analysis.

The platform allowed the team to track activity in dendrites before, during, and several minutes after the neuron activates, capturing the moments critical to protein synthesis in the cell and, more importantly, the stage considered key to memory formation.

An analysis of these crucial moments revealed a microscopic upheaval in the dendrite. Upon activation, local ribosomes jump onto mRNAs, an action that has all the biochemical hallmarks of memory formation, and which models predicted will cause the dendrite to produce not only new proteins, but 1,000 small proteins known as micropeptides, with as-yet unknown function.

The team also identified an RNA-binding protein that helps seal the connection between these ribosomes and mRNA, and demonstrated that if that protein is disabled, the proposed micropeptides will not form.

“We never knew these micropeptides might even exist,” Darnell says.

“It opens a new field of study, where we can ask what these peptides might be doing and how they could play into memory formation. It’s such a vast discovery that there are dozens if not hundreds of avenues in which to pursue this.”

Among the many observations that researchers will unpack in future studies, one stood out: the team noted that a certain protein stood out for its prolific binding of mRNA in the dendrite.

The protein, called FMRP, is key to brain development and function, and genetic mutations that adversely impact FMRP contribute to Fragile X syndrome, one of the most common genetic causes of intellectual disability.

“Our findings fit nicely with the molecular biology of FMRP, and also open the door to future insights into what is going wrong in Fragile X,” Darnell says.

Beyond the paper’s immediate findings, dendritic-TurboID could also allow researchers to examine protein synthesis in other brain regions and apply the findings to different diseases.

“We can now begin to look at many other sites with a fine-toothed comb,” Hacisuleyman says.

“When you develop a new technique as Hacisuleyman did, you enter a room that nobody has ever been in before,” Darnell adds. “The light turns on, and the findings just take your breath away.”

About this genetics, memory, and learning research news

Author: Katherine Fenz
Source: Rockefeller University
Contact: Katherine Fenz – Rockefeller University
Image: The image is credited to Neuroscience News

Original Research: Open access.
Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding” by Robert B. Darnell et al. Nature Neuroscience


Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding

Learning and memory require activity-induced changes in dendritic translation, but which mRNAs are involved and how they are regulated are unclear.

In this study, to monitor how depolarization impacts local dendritic biology, we employed a dendritically targeted proximity labeling approach followed by crosslinking immunoprecipitation, ribosome profiling and mass spectrometry.

Depolarization of primary cortical neurons with KCl or the glutamate agonist DHPG caused rapid reprogramming of dendritic protein expression, where changes in dendritic mRNAs and proteins are weakly correlated.

For a subset of pre-localized messages, depolarization increased the translation of upstream open reading frames (uORFs) and their downstream coding sequences, enabling localized production of proteins involved in long-term potentiation, cell signaling and energy metabolism.

This activity-dependent translation was accompanied by the phosphorylation and recruitment of the non-canonical translation initiation factor eIF4G2, and the translated uORFs were sufficient to confer depolarization-induced, eIF4G2-dependent translational control.

These studies uncovered an unanticipated mechanism by which activity-dependent uORF translational control by eIF4G2 couples activity to local dendritic remodeling.

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