1Summary: After creating a memory in a context, engram cells encoding the memory in the hippocampus become more excitable when the context is repeated a short time later, researchers report.Source: MIT.Whether it’s the pleasant experience of returning to one’s childhood home over the holidays or the unease of revisiting a site that proved unpleasant, we often find that when we return to a context where an episode first happened, specific and vivid memories can come flooding back. In a new study in Neuron, scientists in MIT’s Picower Institute for Learning and Memory report the discovery of a mechanism the brain may be employing to make that phenomenon occur.“Suppose you are driving home in the evening and encounter a beautiful orange twilight in the sky, which reminds you of the great vacation you had a few summers ago at a Caribbean island,” said study senior author Susumu Tonegawa, Picower Professor of Neuroscience at MIT. “This initial recall could be a general recall of the vacation. But moments later, you may get reminded of details of some specific events or situations that took place during the vacation which you had not been thinking about.”At the heart of that second stage of recall, where specific details are suddenly vividly available, is a change in the electrical excitability of “engram cells,” or the ensemble of neurons that together encode a memory through the specific pattern of their connection. In the new study, Tonegawa’s lab, led by postdoc Michele Pignatelli and former member Tomas Ryan, now at Trinity College Dublin, showed that after mice formed a memory in a context, the engram cells encoding that memory in a brain region called the hippocampus would temporarily become much more electrically excitable if the mice were placed back in the same context again. So for instance, if they were given a little shock in a specific context one day, then the engram cells would be much more excitable for about an hour after they were put back in that same context the next day.The specific change in the engram cells’ electrical properties has some direct implications for learning and behavior that hadn’t been appreciated before. Importantly, during that hour after returning to the initial context, because of the engrams’ elevated excitability, mice proved better able to learn from a shock in that context and better able to distinguish between that and distinct contexts even if they shared some similar cues. The increase in excitability therefore allowed them both to learn to avoid places where danger happened very recently and to continue to function normally in places that happen to have some irrelevant resemblance. And because the effect was short-lived, it didn’t oblige them to remain overly attuned for very long.“The short-term reactivation increases the future recognition capability of specific cues,” Pignatelli and Tonegawa’s team wrote. “Engram cell excitability may be crucial for survival by facilitating rapid adaptive behavior without permanently altering the fundamental nature of the long-term engram.”Tonegawa added that “while the survival interpretation may be an evolutionary origin of this multi-step episodic memory recall” it likely also applies to positive episodic memories, like the vacation sunset experience, just as much.Exposing excitabilityUsing methods they’ve devised to label specific engrams, the team initially explored what happens when mice are returned to the context where they previously formed a new memory. They found that five minutes after mice were re-exposed to the context, the engram cells in the dentate gyrus of the hippocampus were more electrically excitable than in mice who had been re-exposed three hours before or in mice who weren’t re-exposed at all. Subsequent experiments showed that the added excitability lasted for an hour after re-exposure before dwindling to no difference by two hours.The finding prompted the scientists to investigate what accounted for the change. Their examination of the engram cells revealed that when they were more excitable, they expressed fewer “inward rectifier” channels for potassium ions on their surface.Meanwhile, if they chemically interfered with protein synthesis, they found they could prevent the excitability from returning back to normal levels after an hour. Also, if the induced extra surface expression of the ion channels, they prevented re-exposure to a context from increasing excitability. Taken together, their findings suggested that reduced expression of the potassium ion channels causes the excitability increase and protein synthesis ends it (after about an hour).Behavioral batteryTo understand the implications of the extra excitement on behavior, the team performed experiments that tested its effect on two main strategies of memory recall: pattern separation, in which the brain distinguishes between cues, and pattern completion, in which the brain extrapolates from available cues.To test separation, they first trained the mice by giving them a little shock while in a specific place (context A). They next day they re-exposed two groups of mice to context A (to increase engram excitability) but left a third group out of context A as an unexcited control. The next day, they exposed all the mice to a new context that shared some of A’s cues but also had other ones (Context AB). Some of the mice that had been returned to Context A entered context AB after five minutes, while others entered after three hours.So what happened? The mice who didn’t go back to A at all and the mice who had three hours between A and AB each froze up in fear in AB, as if they couldn’t be sure they weren’t back in A (where they had experienced a shock the day before). The mice who went to AB only five minutes after being in A didn’t freeze up in AB. Well within their hour of elevated engram excitability, they could tell the difference between A and AB.On the first day of the pattern completion test, the researchers gave three different groups of mice about 10 minutes to explore a context (Context A). They next day they returned all three groups to context A. The control group got a little shock and were removed immediately. The second and third groups each got to spend 3 minutes re-exploring context A. The second group then returned again five minutes later for a mild shock and the third group returned three hours later to get a shock.Red staining in engram cells in the hippocampus indicate expression of (Kir2.1) potassium ion channels. NeuroscienceNews.com image is credited to Pignatelli, Tonegawa, et al.On day 3 all three groups were returned to Context A. The control mice and the ones who received the shock three hours after re-exposure each were much less likely to freeze in fear than the mice who got their shock within five minutes of re-exposure. They were clearly more attuned to being back in A.The scientists hypothesize that the way enhanced excitability sharpens memory is by making the engram cells more sensitive to lower levels of input from their connections with other neurons, or synapses. In the broader circuits that connect memory and behavior, they are relays that can act even with less information.“The excitability increase in the engram cell is likely to compensate for the reduction of synaptic inputs due to limited cue availability,” they wrote.See alsoFeaturedGeneticsNeuroscienceOpen Neuroscience Articles·April 22, 2020Cells in lungs, nasal passage, and intestines more susceptible to COVID-19 infection[divider]About this neuroscience research article[/divider]In addition to Pignatelli, Ryan and Tonegawa, the paper’s other authors are Dheeraj Roy, Chanel Lovett, Lillian Smith and Shruti Muralidhar.Funding: The RIKEN Brain Science Institute, the Howard Hughes Medical Institute and the JPB Foundation funded the study.Source: David Orenstein – MIT Publisher: Organized by NeuroscienceNews.com. Image Source: NeuroscienceNews.com image is credited to Pignatelli, Tonegawa, et al. Original Research: Abstract for “Engram Cell Excitability State Determines the Efficacy of Memory Retrieval” by Michele Pignatelli, Tomás J. Ryan, Dheeraj S. Roy, Chanel Lovett, Lillian M. Smith, Shruti Muralidhar, and Susumu Tonegawa in Neuron. Published December 11 2018. doi:10.1016/j.neuron.2018.11.029[divider]Cite This NeuroscienceNews.com Article[/divider][cbtabs][cbtab title=”MLA”]MIT”How Returning to a Prior Context Briefly Heightens Memory Recall.” NeuroscienceNews. NeuroscienceNews, 11 December 2018. <https://neurosciencenews.com/context-memory-recall-10325/>.[/cbtab][cbtab title=”APA”]MIT(2018, December 11). How Returning to a Prior Context Briefly Heightens Memory Recall. NeuroscienceNews. Retrieved December 11, 2018 from https://neurosciencenews.com/context-memory-recall-10325/[/cbtab][cbtab title=”Chicago”]MIT”How Returning to a Prior Context Briefly Heightens Memory Recall.” https://neurosciencenews.com/context-memory-recall-10325/ (accessed December 11, 2018).[/cbtab][/cbtabs]AbstractEngram Cell Excitability State Determines the Efficacy of Memory RetrievalAnimals need to optimize the efficacy of memory retrieval to adapt to environmental circumstances for survival. The recent development of memory engram labeling technology allows a precise investigation of the processes associated with the recall of a specific memory. Here, we show that engram cell excitability is transiently increased following memory reactivation. This short-term increase of engram excitability enhances the subsequent retrieval of specific memory content in response to cues and is manifest in the animal’s ability to recognize contexts more precisely and more effectively. These results reveal a hitherto unknown transient enhancement of context recognition based on the plasticity of engram cell excitability. They also suggest that recall of a contextual memory is influenced by previous but recent activation of the same engram. The state of excitability of engram cells mediates differential behavioral outcomes upon memory retrieval and may be crucial for survival by promoting adaptive behavior.[divider]Feel free to share this Neuroscience News.[/divider]Join our Newsletter I agree to have my personal information transferred to AWeber for Neuroscience Newsletter ( more information )Sign up to receive the latest neuroscience headlines and summaries sent to your email daily from NeuroscienceNews.comWe hate spam and only use your email to contact you about newsletters. We do not sell email addresses. You can cancel your subscription any time.