Neurotransmission Caught on Camera

Scientists have captured the exact point and time when information is exchanged between brain cells, a breakthrough that could explain how and why neurological conditions like schizophrenia or epilepsy occur.

An international team, led by Professor Rory Duncan at Heriot-Watt University, has used new ‘super-resolution’ microscopy or ‘nanoscopy’ techniques to understand how synapses function and ultimately transmit information throughout the brain and nervous system.

These new results, published in Nature Communications, will lead to better understanding of synapses, the structures in nerve cells in the brain, at the single molecule level and perhaps pinpoint where and when certain neurological conditions begin. These molecules drive neurotransmission and the new microscope techniques allow them to be seen in action for the first time.

Professor Rory Duncan, an expert in molecular membrane biology and microscopy at Heriot-Watt University, said, “Until now, nobody has understood exactly how neurotransmission worked, because the techniques to analyse it were limited.

“We’ve developed sophisticated microscope methods to determine how synapses are regulated and function, in real time and with massive accuracy. The molecules we can see, and track in their thousands using mathematical approaches, are as small to us as Jupiter is large; these new ‘super-resolution’ techniques mean that biologists can at last see the things that make cells work.

The image shows the microscopy images taken as part of the research.
Researchers have used new ‘super-resolution’ microscopy or ‘nanoscopy’ techniques to understand how synapses function and ultimately transmit information throughout the brain and nervous system. Image adapted from the Heriot-Watt University press release.

“Once we understand how synapses function, in terms of the how, where and when of events, we will know what needs to change in order potentially to treat neurological disorders such as schizophrenia.”

The team’s new approaches use a distinctive combination of laser physics, mathematics, genetics and cell biology, ‘fusing’ genes from fluorescent jellyfish and corals to the genes that make the molecular drivers which underlie neurotransmission. Shining lasers on the proteins these fluorescent ‘fusion proteins’ make in living synapses allows the molecules to be seen in action.

About this electrophysiology research

Professor Rory Duncan is Head of the Institute of Biological Chemistry, Biophysics and Bioengineering at Heriot-Watt University, as well as of the Edinburgh super-resolution Imaging Consortium (ESRIC), an MRC-funded consortium of cell biologists and physical scientists involving Heriot-Watt and Edinburgh University, which drives the development of these new techniques.

This research was funded by the Wellcome Trust and UK Medical Research Council.

Contact: Rory Duncan – Heriot-Watt University
Source: Heriot-Watt University press release
Image Source: The image is credited to the researchers and is adapted from the Heriot-Watt University press release
Original Research: Full open access research for “A molecular toggle after exocytosis sequesters the presynaptic ​syntaxin1a molecules involved in prior vesicle fusion” by Deirdre M. Kavanagh, Annya M. Smyth, Kirsty J. Martin, Alison Dun, Euan R. Brown, Sarah Gordon, Karen J. Smillie, Luke H. Chamberlain, Rhodri S. Wilson, Lei Yang, Weiping Lu, Michael A. Cousin, Colin Rickman and Rory R. Duncan in Nature Communications. Published online December 17 2014 doi:10.1038/ncomms6774

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