Summary: Researchers have identified the structure of a protein involved in inflammation and implicated in Parkinson’s disease.
Source: Saint Louis University.
In a recent paper published in Nature Communications, Saint Louis University scientists report that they have determined the structure of a key protein that is involved in the body’s inflammatory response. This finding opens the door to developing new treatments for a wide range of illnesses, from heart disease, diabetes and cancer to neurodegenerative disorders, including Parkinson’s disease.
Sergey Korolev, Ph.D., associate professor of biochemistry and molecular biology at SLU, studies protein structures at the atomic resolution level to understand mechanism of their function in the body.
Korolev and his team examined a long-studied but little-understood enzyme, calcium-independent phospholipase A2β, (iPLA2β) that cleaves phospholipids in membrane. It produces important signals after injury to initiate the inflammatory response. The team wanted to know how the enzyme is activated during injury, how it hydrolyses substrates and how it gets shut down, turning the inflammatory response off.
Korolev says that the protein kept popping up in seemingly unrelated areas of study throughout the last few decades.
“It was first discovered more than 20 years ago at Washington University in Richard Gross’s lab,” Korolev said. “They found that the protein played a role as a part of the cardiovascular system in response to an ischemia or injury.
“Next, researchers found that it is also involved in the insulin production cycle and, when misregulated, can lead to type I diabetes. Then, less than 10 years ago, it was rediscovered from a completely different point of view through the genetic sequencing of patients with neurodegenerative issues. For example, inherited mutations in this gene were identified in patients with early onset Parkinson’s.”
For this reason, the protein also has a second name, PARK14, due to numerous inherited mutations of this gene that were identified in patients with early Parkinson’s.
Researchers saw that the protein played different roles in different tissues and parts of the cell. The protein’s changeable roles added to difficulties in understanding how it operated.
It was clear to scientists, though, that the action of the protein can be harmful, contributing to cardiovascular diseases, diabetes and cancer metastasis, and many investigators attempted to design inhibitors to serve as potential new therapies.
“Different groups tried to design inhibitors, but it was very difficult without knowing the 3D structure of the protein,” Korolev said.
In order to learn more about the protein’s molecular structure, SLU researchers used x-ray crystallography to gather data.
The process involves growing a crystal of the protein, shooting x-ray beams through the crystal and analyzing the diffraction pattern generated on a detector plate in order to detail the three-dimensional structure of the protein.
Often, the most difficult part of the process protein crystallization, which can take years to achieve.
With his team’s success in discovering the protein’s structure, Korolev anticipates that the door has been opened to answer many more questions about the protein.
“Before we had the structure, people didn’t have good tools to study this enzyme,” Korolev said. “Now, this will take the field to a new level.
“We’ve opened up lots of possibilities. The mechanism of regulation was completely unknown. Now, the 3D structure gives us a clear hypothesis for how it is responsible for action in different cellular compartments and tissues.
“Now that we can better understand how the protein interacts with lipid molecules, it will be much easier to develop drugs.”
Korolev notes that the structure his team discovered was quite different from what researchers predicted the protein might look like.
“One of the key findings about the structure we uncovered is that it significantly revised previously developed theoretical models, which couldn’t explain many functional features. Now, with the real structure, many pieces of an intricate puzzle click together providing clear hypothesis about the mechanism of protein’s function and regulation.”
In addition, Korolev is intrigued by the protein’s function in the brain, which is completely unknown. Thanks to genetic sequencing, researchers can now map out which parts of a protein cause diseases. Having the genetic information together with the 3D structure will offer researchers a powerful new tool.
“This has been a long project,” Korolev said. “It turned out that this enzyme was difficult to work with. It was challenging to get funding. Our collaborators dropped out. We were supported for a long time with a low budget because the department kept us afloat. SLU undergrads helped us. We received some funding from NASA to try crystallization under microgravity conditions and a small NIH grant.
“Most recently, our M.D./Ph.D. student, Konstantin Malley, who authored the paper, worked really hard on this research.
“In the past, people have studied this complex enzyme, like a black box, without knowing what is inside,” Korolev said. Now that we have discovered the structure, we can see every atom. This allows us to visualize what is happening with this protein. It is a completely new level of insight.”
Malley hopes the findings will prove to be a helpful step forward in the development of new therapies for neurodegenerative disease.
“There is a growing amount of genetic work that links iPLA2β to neurodegenerative disease, and physicians and scientists worldwide are now interested in its function,” Malley said. “We are still a long way from treating patients, but I would like them to know that the structure is a large step between genetics and developing targeted therapies for treatment.
“We hope that it provides a jumping-off point for firstly, understanding how iPLA2β works in the brain. Next we can employ different strategies, such as small molecule drugs, that would either prevent iPLA2β interacting with other proteins, or change its activity to prevent inflammation, which is an increasingly important factor in Parkinson’s and other brain disorders.”
Other researchers on the study include Olga Koroleva, Ian Miller, Ruslan Sanishvili, Christopher M. Jenkins and Richard W. Gross.
Funding: American Heart Association Grant-in-Aid, Center for Advancement of Science in Space, National Institutes of Health funded this study.
Source: Carrie Bebermeyer – Saint Louis University
Publisher: Organized by NeuroscienceNews.com.
Image Source: NeuroscienceNews.com image is credited to Korolev et al./Nature Communications.
Original Research: Open access research in Nature Communications.
The structure of iPLA2β reveals dimeric active sites and suggests mechanisms of regulation and localization
Calcium-independent phospholipase A2β (iPLA2β) regulates important physiological processes including inflammation, calcium homeostasis and apoptosis. It is genetically linked to neurodegenerative disorders including Parkinson’s disease. Despite its known enzymatic activity, the mechanisms underlying iPLA2β-induced pathologic phenotypes remain poorly understood. Here, we present a crystal structure of iPLA2β that significantly revises existing mechanistic models. The catalytic domains form a tight dimer. They are surrounded by ankyrin repeat domains that adopt an outwardly flared orientation, poised to interact with membrane proteins. The closely integrated active sites are positioned for cooperative activation and internal transacylation. The structure and additional solution studies suggest that both catalytic domains can be bound and allosterically inhibited by a single calmodulin. These features suggest mechanisms of iPLA2β cellular localization and activity regulation, providing a basis for inhibitor development. Furthermore, the structure provides a framework to investigate the role of neurodegenerative mutations and the function of iPLA2β in the brain.