Summary: A new study reports the interaction between a mitochondrial protein called TOM20 and alpha-synuclein leads to neurodegeneration in Parkinson’s disease.
Source: University of Pittsburgh School of Medicine.
Researchers at the University of Pittsburgh School of Medicine have uncovered a major reason why the Parkinson’s-related protein alpha-synuclein, a major constituent of the Lewy bodies that are the pathological hallmark of Parkinson’s disease (PD), is toxic to neurons in the brain. The finding has the potential to lead to new therapies that could slow or stop progression of the devastating illness. The new research appears online today in Science Translational Medicine.
PD is a degenerative neurological disease characterized by tremor, slowness, and gait and balance difficulties that affects about 1 million people in the United States. The symptoms are caused by the degeneration and loss of neurons in the brain, particularly those crucial for the initiation and coordination of movement.
“It’s really exciting that we have found a mechanism we can target to create new treatments for this devastating disease,” said lead investigator J. Timothy Greenamyre, M.D., Ph.D., Love Family Professor of Neurology in Pitt’s School of Medicine and director of the Pittsburgh Institute for Neurodegenerative Diseases (PIND).
PIND’s goal is an integrated, interdisciplinary approach to the study of neurodegenerative diseases and their mechanisms, with the aim of transforming cutting-edge science into novel therapies and diagnostics that directly benefit individuals affected by neurodegenerative diseases.
“With four different PIND investigators working together, the new study highlights the power of this collaborative approach,” Dr. Greenamyre added.
Current treatments for PD can reduce symptoms, but they do not slow the inevitable worsening of the disease. To slow or halt illness progression, scientists must first determine why and how the neurons are dying.
Degenerating neurons contain large clumps of a protein called alpha-synuclein. People whose cells make too much alpha-synuclein or make a mutated form of the protein are at high risk of developing PD because of the protein’s toxicity, researchers found. Scientists also demonstrated that the accumulation of alpha-synuclein in PD is toxic because it disrupts the normal functioning of mitochondria–the tiny powerhouses responsible for generating a cell’s energy.
In the new study, Dr. Greenamyre and his team–led by coauthors Roberto Di Maio, Ph.D., and Paul Barrett, Ph.D., both of PIND–used a well-established rodent model of PD to show exactly how alpha-synuclein disrupts mitochondrial function. They found that by attaching to a mitochondrial protein called TOM20, alpha-synuclein prevented the mitochondria from functioning optimally, which resulted in the production of less energy and more damaging cellular waste.
Ultimately, this interaction between alpha-synuclein and TOM20 leads to neurodegeneration, Dr. Greenamyre explained.
The researchers then confirmed their animal findings in brain tissue from people with PD.
“The effects of alpha-synuclein on mitochondria are like making a perfectly good coal-fueled power plant extremely inefficient, so it not only fails to make enough electricity, but also creates too much toxic pollution,” said Dr. Greenamyre.
Using cell cultures, the research team also found two ways to prevent the toxicity caused by alpha-synuclein: gene therapy that forced the neurons to make more TOM20 protein protected them from the alpha-synuclein; and a protein that was able to prevent alpha-synuclein from sticking to TOM20 prevented alpha-synuclein’s harmful effects on mitochondria.
While more research is needed to determine whether these approaches could help PD patients, Dr. Greenamyre is optimistic that one or both may ultimately make it into human clinical trials in an effort to slow or halt the otherwise inevitable progression of PD.
Coauthors of the study are Charleen Chu, M.D., Ph.D., Edward Burton, M.D., Ph.D., Teresa Hastings, Ph.D., Eric Hoffman, Ph.D., Caitlyn Barrett, Ph.D., Alevtina Zharikov, Ph.D., Anupom Borah, Ph.D., Xiaoping Hu, B.S., and Jennifer McCoy, B.S., all of PIND.
Funding: This work was supported by research grants from the DSF Charitable Foundation, the Ri.MED Foundation, the Consolidated Anti-Aging Foundation, the National Institutes of Health (grants NS095387, NS059806, ES022644, ES020718, ES020327, NS065789, AG026389 and P50AG005133), the United States Department of Veterans’ Affairs (grant 1I01BX000548), the Blechman Foundation, the American Parkinson Disease Association and the Department of Biotechnology, Government of India.
Source: Gloria Kreps – University of Pittsburgh School of Medicine
Image Source: This NeuroscienceNews.com image is credited to Marvin 101 and is licensed CC BY-SA 3.0.
Original Research: Abstract for “α-Synuclein binds to TOM20 and inhibits mitochondrial protein import in Parkinson’s disease” by Roberto Di Maio, Paul J. Barrett, Eric K. Hoffman, Caitlyn W. Barrett, Alevtina Zharikov1, Anupom Borah, Xiaoping Hu, Jennifer McCoy, Charleen T. Chu, Edward A. Burton, Teresa G. Hastings and J. Timothy Greenamyre in Science Translational Medicine. Published online June8 2016 doi:10.1126/scitranslmed.aaf3634
[cbtabs][cbtab title=”MLA”]University of Pittsburgh School of Medicine. “A Key to Parkinson’s Neurodegeneration Identified.” NeuroscienceNews. NeuroscienceNews, 8 June 2016.
<https://neurosciencenews.com/alpha-synuclein-mitochondria-parkinsons-4411/>.[/cbtab][cbtab title=”APA”]University of Pittsburgh School of Medicine. (2016, June 8). A Key to Parkinson’s Neurodegeneration Identified. NeuroscienceNews. Retrieved June 8, 2016 from https://neurosciencenews.com/alpha-synuclein-mitochondria-parkinsons-4411/[/cbtab][cbtab title=”Chicago”]University of Pittsburgh School of Medicine. “A Key to Parkinson’s Neurodegeneration Identified.” https://neurosciencenews.com/alpha-synuclein-mitochondria-parkinsons-4411/ (accessed June 8, 2016).[/cbtab][/cbtabs]
Abstract
α-Synuclein binds to TOM20 and inhibits mitochondrial protein import in Parkinson’s disease
α-Synuclein accumulation and mitochondrial dysfunction have both been strongly implicated in the pathogenesis of Parkinson’s disease (PD), and the two appear to be related. Mitochondrial dysfunction leads to accumulation and oligomerization of α-synuclein, and increased levels of α-synuclein cause mitochondrial impairment, but the basis for this bidirectional interaction remains obscure. We now report that certain posttranslationally modified species of α-synuclein bind with high affinity to the TOM20 (translocase of the outer membrane 20) presequence receptor of the mitochondrial protein import machinery. This binding prevented the interaction of TOM20 with its co-receptor, TOM22, and impaired mitochondrial protein import. Consequently, there were deficient mitochondrial respiration, enhanced production of reactive oxygen species, and loss of mitochondrial membrane potential. Examination of postmortem brain tissue from PD patients revealed an aberrant α-synuclein–TOM20 interaction in nigrostriatal dopaminergic neurons that was associated with loss of imported mitochondrial proteins, thereby confirming this pathogenic process in the human disease. Modest knockdown of endogenous α-synuclein was sufficient to maintain mitochondrial protein import in an in vivo model of PD. Furthermore, in in vitro systems, overexpression of TOM20 or a mitochondrial targeting signal peptide had beneficial effects and preserved mitochondrial protein import. This study characterizes a pathogenic mechanism in PD, identifies toxic species of wild-type α-synuclein, and reveals potential new therapeutic strategies for neuroprotection.
“α-Synuclein binds to TOM20 and inhibits mitochondrial protein import in Parkinson’s disease” by Roberto Di Maio, Paul J. Barrett, Eric K. Hoffman, Caitlyn W. Barrett, Alevtina Zharikov1, Anupom Borah, Xiaoping Hu, Jennifer McCoy, Charleen T. Chu, Edward A. Burton, Teresa G. Hastings and J. Timothy Greenamyre in Science Translational Medicine. Published online June8 2016 doi:10.1126/scitranslmed.aaf3634
